LOCUS       Plasmid_-_pBSII-NLS-mCherry(AM)        4655 bp    DNA     circular UNA 31-JUL-2025
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local.frstrobl.88y-gv43278
VERSION     urn.local.frstrobl.88y-gv43278
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        626..645
                     /modified_by="frstrobl"
                     /label="T7 Promoter"
     ORF             723..749
                     /modified_by="frstrobl"
                     /label="NLS ORF"
     misc_feature    750..757
                     /modified_by="frstrobl"
                     /label="NotI"
                     /note="Geneious type: Important RE Site"
     ORF             759..1463
                     /modified_by="frstrobl"
                     /label="mCherry(AM) ORF"
     misc_feature    1464..1471
                     /modified_by="frstrobl"
                     /label="SbfI"
                     /note="Geneious type: Important RE Site"
     polyA_signal    1472..2448
                     /modified_by="frstrobl"
                     /label="SV40 Poly(A)"
     rep_origin      2893..3507
                     /modified_by="frstrobl"
                     /label="Origin of Replication"
     ORF             complement(3667..4527)
                     /modified_by="frstrobl"
                     /label="Beta-Lactamase (AmpR) ORF"
     promoter        complement(4528..4632)
                     /modified_by="frstrobl"
                     /label="Beta-Lactamase Promoter"
ORIGIN      
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccgggca
      721 aaatggcccc caagaagaaa aggaaagtgg cggccgcctc gaaaggcgaa gaagataaca
      781 tggccattat taaagaattc atgagattca aagtgcacat ggaaggctcg gtgaacggcc
      841 acgaattcga aattgaaggc gaaggcgaag gcagaccata cgaaggcacc caaaccgcca
      901 aactgaaagt gaccaaaggc ggcccactgc cattcgcctg ggacattctg tcgccacaat
      961 tcatgtacgg ctcgaaagcc tacgtgaaac acccagccga cattccagac tacctgaaac
     1021 tgtcgttccc agaaggcttc aaatgggaaa gagtgatgaa cttcgaagat ggcggcgtgg
     1081 tgaccgtgac ccaagactcg tcgctgcaag acggcgaatt catttacaaa gtgaaactga
     1141 gaggcaccaa cttcccatcg gacggcccag tgatgcaaaa gaaaaccatg ggctgggaag
     1201 cctcgtcgga aagaatgtac ccagaagatg gcgccctgaa aggcgaaatt aaacaaagac
     1261 tgaaactgaa agacggcggc cactacgacg ccgaagtgaa aaccacctac aaagccaaga
     1321 aaccagtgca actgccaggc gcctacaacg tgaacattaa actggacatt acctcgcaca
     1381 acgaagatta caccattgtg gaacaatacg aaagagccga aggcagacac tcgaccggcg
     1441 gcatggacga actgtacaaa taacctgcag ggatcataat cagccatacc acatttgtag
     1501 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     1561 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1621 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1681 aactcatcaa tgtatcttat catgtctgga tccccaggaa gctcctctgt gtcctcataa
     1741 accctaacct cctctacttg agaggacatt ccaatcatag gctgcccatc caccctctgt
     1801 gtcctcctgt taattaggtc acttaacaaa aaggaaattg ggtaggggtt tttcacagac
     1861 cgctttctaa gggtaatttt aaaatatctg ggaagtccct tccactgctg tgttccagaa
     1921 gtgttggtaa acagcccaca aatgtcaaca gcagaaacat acaagctgtc agctttgcac
     1981 aagggcccaa caccctgctc atcaagaagc actgtggttg ctgtgttagt aatgtgcaaa
     2041 acaggaggca cattttcccc acctgtgtag gttccaaaat atctagtgtt ttcattttta
     2101 cttggatcag gaacccagca ctccactgga taagcattat ccttatccaa aacagccttg
     2161 tggtcagtgt tcatctgctg actgtcaact gtagcatttt ttggggttac agtttgagca
     2221 ggatatttgg tcctgtagtt tgctaacaca ccctgcagct ccaaaggttc cccaccaaca
     2281 gcaaaaaaat gaaaatttga cccttgaatg ggttttccag caccattttc atgagttttt
     2341 tgtgtccctg aatgcaagtt taacatagca gttaccccaa taacctcagt tttaacagta
     2401 acagcttccc acatcaaaat atttccacag gttaagtcct catttaaaga gctccagctt
     2461 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     2521 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2581 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2641 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2701 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     2761 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2821 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2881 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2941 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3001 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3061 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3121 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3181 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3241 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3301 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     3361 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3421 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3481 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3541 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3601 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3661 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3721 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3781 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3841 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3901 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3961 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4021 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4081 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4141 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4201 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4261 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4321 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4381 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4441 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4501 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4561 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4621 aggggttccg cgcacatttc cccgaaaagt gccac
//
