LOCUS       Exported                8685 bp ds-DNA     circular UNA 29-JUL-2025
DEFINITION  natural circular DNA
ACCESSION   urn.local...7n-jgybmxt
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             complement(join(963..1031,171..962))
                     /codon_start=1
                     /gene="bla"
                     /standard_name="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*MSIQHFRVALIPFFAAF
                     CLPVFA"
     regulatory      complement(1032..1136)
                     /gene="bla"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
                     /note="Geneious type: promoter"
     regulatory      1167..1546
                     /standard_name="CMV enhancer"
                     /label=CMV enhancer
                     /note="Geneious type: enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      1548..1827
                     /standard_name="chicken beta-actin promoter"
                     /label=chicken beta-actin promoter
                     /note="Geneious type: promoter"
     intron          1828..2844
                     /standard_name="chimeric intron"
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     regulatory      2937..2946
                     /regulatory_class="other"
                     /standard_name="Kozak sequence"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Koza k, 1987)"
     CDS             2949..2969
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2979..2999
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3012..6692
                     /codon_start=1
                     /standard_name="LbCpf1"
                     /product="CRISPR-associated protein from
                     Lachnospiraceae bacterium ND2006 (Zets che et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             6693..6740
                     /codon_start=1
                     /standard_name="nucleoplasmin NLS"
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6759..6779
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6934..6989
                     /standard_name="beta-globin poly(A) signal"
                     /label=beta-globin poly(A) signal
                     /note="Geneious type: polyA_signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7350..7366)
                     /standard_name="M13 rev"
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7374..7390
                     /standard_name="lac operator"
                     /label=lac operator binding site
                     /bound_moiety="lac repressor encoded by
                     lacI"
                     /note="The lac repressor binds to the lac
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(join(7423..7428,7405..7422,7398..7404))
                     /standard_name="lac promoter"
                     /label=lac promoter
                     /note="Geneious type: promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7443..7464
                     /standard_name="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator
                     protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      7523..7718
                     /standard_name="SV40 promoter"
                     /label=SV40 promoter
                     /note="Geneious type: promoter"
                     /note="SV40 early promoter"
     rep_origin      7569..7704
     misc_feature    7724..7858
                     /standard_name="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="Geneious type: polyA_signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8097..8685)
                     /direction=LEFT
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc
     1201 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1261 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1321 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1381 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1441 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1501 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     1561 cacgttctgc ttcactctcc ccatctcccc ccccctcccc acccccaatt ttgtatttat
     1621 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg gcgcgcgcca
     1681 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
     1741 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
     1801 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
     1861 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     1921 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     1981 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     2041 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     2101 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     2161 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     2221 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     2281 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     2341 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     2401 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     2461 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     2521 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     2581 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     2641 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     2701 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     2761 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     2821 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     2881 cattttggca aagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     2941 ccatgggccc aaagaaaaag aggaaagtcg gcagtggacc taaaaagaaa cgaaaggttg
     3001 ggtcaggttc tagcaagctg gaaaaattta ccaactgtta cagcctgagc aaaaccctga
     3061 ggttcaaggc catccccgtg ggcaagaccc aagagaacat cgataacaag agactgctgg
     3121 tcgaggatga gaagcgtgca gaggactaca agggcgtgaa gaagctcctg gatcggtact
     3181 acctgagttt catcaacgac gtgctgcaca gcatcaagct gaagaacctg aacaactaca
     3241 tcagcctgtt tagaaagaaa accagaaccg aaaaagaaaa taaggagctg gaaaatctgg
     3301 aaatcaacct gagaaaggaa atcgctaagg cctttaaggg aaacgaagga tacaaatccc
     3361 tgtttaagaa agacatcatc gagacaattc tgccagagtt cctggacgac aaagacgaga
     3421 tagctcttgt gaacagcttc aacggcttca cgaccgcctt caccggattc ttcgacaaca
     3481 gggaaaacat gttcagtgag gaagccaagt ctaccagcat cgctttccgg tgcatcaatg
     3541 agaacctgac ccggtatatc tctaatatgg atatctttga aaaagtggac gctattttcg
     3601 acaagcacga ggtgcaggag atcaaggaga agatcctgaa cagcgactac gacgtggagg
     3661 acttcttcga gggcgaattt ttcaacttcg tgctgacaca agagggcatc gacgtttaca
     3721 acgccatcat aggcggcttc gtgaccgagt ccggcgaaaa gatcaagggc cttaacgagt
     3781 acatcaacct gtataaccag aaaaccaagc agaagctgcc taagttcaag cctctttata
     3841 agcaggtcct gagcgaccgg gaaagcctga gcttctacgg cgagggctac acatctgacg
     3901 aggaagtgct ggaggtcttt cggaataccc tgaacaagaa ctctgagatc ttcagctcta
     3961 ttaagaaact ggaaaagctg ttcaagaatt tcgacgagta tagtagcgcc gggatcttcg
     4021 tgaagaacgg ccctgccatt agcaccatca gcaaggacat cttcggcgaa tggaacgtta
     4081 tccgcgataa gtggaacgcc gagtacgatg acatccacct gaaaaagaag gccgtggtga
     4141 ccgagaagta cgaagatgac agaagaaaga gcttcaaaaa gatcggctca tttagcctgg
     4201 aacagctgca ggagtacgcc gacgccgacc tgtccgtggt cgaaaagctg aaggaaatta
     4261 tcatccaaaa agtggacgag atctataagg tgtacgggag ctctgagaag ctgttcgacg
     4321 ccgactttgt gctggaaaag tcactgaaaa agaacgacgc tgtggtcgcc atcatgaagg
     4381 atctgctgga ctccgtgaag agcttcgaga actacatcaa ggccttcttc ggcgaaggca
     4441 aggagaccaa ccgggacgag tctttttacg gcgatttcgt gctggcctac gacatcctgc
     4501 tgaaggtgga ccacatctac gacgccatca gaaactacgt gacccagaag ccctacagca
     4561 aagacaagtt taagctgtac ttccagaatc ctcagttcat gggaggatgg gacaaagaca
     4621 aggaaaccga ctaccgggct acaattctga gatacggctc taagtactac cttgcaatca
     4681 tggacaaaaa gtatgccaag tgcctgcaga aaattgataa ggatgacgtg aacggcaact
     4741 acgagaagat caactacaag ctcctgcccg gccccaacaa gatgctgcca aaggtgttct
     4801 tctccaaaaa gtggatggcc tattacaacc cttctgagga tatccagaag atctataaga
     4861 acggaacatt caagaagggc gatatgttca acctgaatga ctgccacaag ctgatcgact
     4921 tctttaaaga cagcatctcc agatacccta agtggtccaa cgcctacgac ttcaacttca
     4981 gcgaaacaga aaagtacaag gacatcgccg gcttctacag agaggtggaa gaacaaggct
     5041 acaaggtcag cttcgagagc gctagcaaga aggaggtgga caagctggtg gaggagggaa
     5101 agctgtacat gttccagatc tacaacaagg atttcagcga caagagccac ggcacaccta
     5161 acctgcacac catgtatttc aagctgctgt ttgacgagaa caaccatggc cagatccggc
     5221 tgtctggcgg cgccgaactg ttcatgcgga gagcctcttt gaagaaggaa gagctggtgg
     5281 tgcaccctgc caatagccct atcgccaaca agaacccaga taacccaaag aaaaccacca
     5341 cactgagcta cgacgtgtac aaagataaga gattcagcga ggatcagtac gagctgcata
     5401 tccctatcgc catcaataag tgtcctaaaa acatcttcaa gatcaatacc gaggtgcggg
     5461 tgttgctgaa gcacgacgat aacccttacg tgatcggaat cgacaggggc gagagaaacc
     5521 tgctgtacat cgtggtggtg gacggcaagg gcaatatcgt ggagcaatac agcctgaatg
     5581 agatcatcaa caatttcaac ggcatccgca taaaaacaga ttaccactct ctgctggata
     5641 agaaagagaa agagcggttt gaggccagac agaactggac cagcatcgag aatatcaaag
     5701 agttaaaggc cggctacatc agccaggtgg tgcacaaaat ctgcgagctg gtggaaaagt
     5761 acgacgctgt gatcgccctt gaggatctaa acagcggctt taagaacagc agagtgaagg
     5821 tagaaaagca ggtgtatcag aagttcgaga agatgctcat cgataagctg aactacatgg
     5881 tggataagaa aagcaacccc tgcgccacag gcggagctct gaagggctac cagatcacca
     5941 acaaattcga gagctttaag agcatgtcga cccagaatgg tttcatcttt tacatccccg
     6001 cctggctcac ctcaaagatc gatcccagca caggcttcgt caacctgctg aaaaccaagt
     6061 acaccagcat cgccgatagc aagaagttta tctcgtcctt cgacagaatc atgtacgtgc
     6121 ccgaggagga cctgttcgag ttcgccctgg actacaagaa cttctcccgg acagatgccg
     6181 actacattaa gaagtggaag ctgtacagct acggaaacag gatcagaatc tttagaaacc
     6241 ccaaaaagaa caacgtattc gattgggagg aagtgtgcct gacctcggcc tacaaggagc
     6301 tgttcaacaa gtacggcatc aactaccagc agggcgacat ccgggccctg ctctgtgaac
     6361 agagcgacaa ggctttctac agcagcttca tggccctgat gagcctgatg ctgcagatga
     6421 gaaacagcat cacaggaaga acagacgtgg actttctgat ctctcctgtg aagaacagcg
     6481 acggcatctt ctacgacagc agaaactatg aagcccagga gaacgcgatc ctgcctaaga
     6541 acgcagacgc caacggcgcc tacaatatcg ccagaaaggt gctgtgggcc attggccagt
     6601 tcaagaaggc tgaagatgag aaactggata aagtgaagat cgccatctcc aacaaggaat
     6661 ggctggaata cgcccagacc agcgtgaagc acaaaaggcc ggcggccacg aaaaaggccg
     6721 gccaggcaaa aaagaaaaag ggatcctctg gtggttctcc caagaagaag aggaaagtct
     6781 aaagcggccg cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
     6841 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
     6901 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     6961 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     7021 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     7081 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     7141 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     7201 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     7261 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     7321 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7381 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7441 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7501 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
     7561 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7621 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7681 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
     7741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     7861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
