LOCUS       Exported                8684 bp ds-DNA     circular UNA 29-JUL-2025
DEFINITION  natural circular DNA
ACCESSION   urn.local...7p-jgybmxt
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             complement(join(963..1031,171..962))
                     /codon_start=1
                     /gene="bla"
                     /standard_name="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*MSIQHFRVALIPFFAAF
                     CLPVFA"
     regulatory      complement(1032..1136)
                     /gene="bla"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
                     /note="Geneious type: promoter"
     regulatory      1167..1546
                     /standard_name="CMV enhancer"
                     /label=CMV enhancer
                     /note="Geneious type: enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      1548..1826
                     /standard_name="chicken beta-actin promoter"
                     /label=chicken beta-actin promoter
                     /note="Geneious type: promoter"
     intron          1827..2843
                     /standard_name="chimeric intron"
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     regulatory      2936..2945
                     /regulatory_class="other"
                     /standard_name="Kozak sequence"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Koza k, 1987)"
     CDS             2948..2968
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2978..2998
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3001..6691
                     /standard_name="Euk_Flex_LbCas12a"
                     /label=Euk_Flex_LbCas12a
     CDS             6692..6739
                     /codon_start=1
                     /standard_name="nucleoplasmin NLS"
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6758..6778
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6933..6988
                     /standard_name="beta-globin poly(A) signal"
                     /label=beta-globin poly(A) signal
                     /note="Geneious type: polyA_signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7349..7365)
                     /standard_name="M13 rev"
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7373..7389
                     /standard_name="lac operator"
                     /label=lac operator binding site
                     /bound_moiety="lac repressor encoded by
                     lacI"
                     /note="The lac repressor binds to the lac
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(join(7422..7427,7404..7421,7397..7403))
                     /standard_name="lac promoter"
                     /label=lac promoter
                     /note="Geneious type: promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7442..7463
                     /standard_name="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator
                     protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      7522..7717
                     /standard_name="SV40 promoter"
                     /label=SV40 promoter
                     /note="Geneious type: promoter"
                     /note="SV40 early promoter"
     rep_origin      7568..7703
     misc_feature    7723..7857
                     /standard_name="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="Geneious type: polyA_signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8096..8684)
                     /direction=LEFT
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc
     1201 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1261 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1321 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1381 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1441 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1501 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     1561 cacgttctgc ttcactctcc ccatctcccc ccccctcccc acccccaatt ttgtatttat
     1621 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag
     1681 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
     1741 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     1801 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
     1861 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
     1921 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
     1981 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
     2041 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
     2101 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
     2161 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
     2221 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
     2281 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     2341 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     2401 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
     2461 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     2521 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
     2581 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
     2641 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     2701 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     2761 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     2821 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     2881 attttggcaa agaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
     2941 catgggccca aagaaaaaga ggaaagtcgg cagtggacct aaaaagaaac gaaaggttgg
     3001 gtcaggttct agcaagctgg aaaaatttac caactgttac agcctgagca aaaccctgag
     3061 gttcaaggcc atccccgtgg gcaagaccca agagaacatc gataacaaga gactgctggt
     3121 cgaggatgag aagcgtgcag aggactacaa gggcgtgaag aagctcctgg atcggtacta
     3181 cctgagtttc atcaacgacg tgctgcacag catcaagctg aagaacctga acaactacat
     3241 cagcctgttt agaaagaaaa ccagaaccga aaaagaaaat aaggagctgg aaaatctgga
     3301 aatcaacctg agaaaggaaa tcgctaaggc ctttaaggga aacgaaggat acaaatccct
     3361 gtttaagaaa gacatcatcg agacaattct gccagagttc ctggacgaca aagacgagat
     3421 agctcttgtg aacagcttca acaggttcac gaccgccttc accggattct tcgacaacag
     3481 ggaaaacatg ttcagtgagg aagccaagtc taccagcatc gctttccggt gcatcaatga
     3541 gaacctgacc gtgtatatct ctaatatgga tatctttgaa aaagtggacg ctattttcga
     3601 caagcacgag gtgcaggaga tcaaggagaa gatcctgaac agcgactacg acgtggagga
     3661 cttcttcgag ggcgaatttt tcaacttcgt gctgacacaa gagggcatcg acgtttacaa
     3721 cgccatcata ggcggcttcg tgaccgagtc cggcgaaaag atcaagggcc ttaacgagta
     3781 catcaacctg tataaccaga aaaccaagca gaagctgcct aagttcaagc ctctttataa
     3841 gcaggtcctg agcgaccggg aaagcctgag cttctacggc gagggctaca catctgacga
     3901 ggaagtgctg gaggtctttc ggaataccct gaacaagaac tctgagatct tcagctctat
     3961 taagaaactg gaaaagctgt tcaagaattt cgacgagtat agtagcgccg ggatcttcgt
     4021 gaagaacggc cctgccatta gcaccatcag caaggacatc ttcggcgaat ggaacgttat
     4081 ccgcgataag tggaacgccg agtacgatga catccacctg aaaaagaagg ccgtggtgac
     4141 cgagaagtac gaagatgaca gaagaaagag cttcaaaaag atcggctcat ttagcctgga
     4201 acagctgcag gagtacgccg acgccgacct gtccgtggtc gaaaagctga aggaaattat
     4261 catccaaaaa gtggacgaga tctataaggt gtacgggagc tctgagaagc tgttcgacgc
     4321 cgactttgtg ctggaaaagt cactgaaaaa gaacgacgct gtggtcgcca tcatgaagga
     4381 tctgctggac tccgtgaaga gcttcgagaa ctacatcaag gccttcttcg gcgaaggcaa
     4441 ggagaccaac cgggacgagt ctttttacgg cgatttcgtg ctggcctacg acatcctgct
     4501 gaaggtggac cacatctacg acgccatcag aaactacgtg acccagaagc cctacagcaa
     4561 agacaagttt aagctgtact tccagaatcc tcagttcatg ggaggatggg gcaaagacaa
     4621 ggaaaccgac taccgggcta caattctgag atacggcttt aagtactacc ttgcaatcat
     4681 ggacaaaaag tatgccaagt gcctgcagaa aattgataag gatgacgtga acggcaacta
     4741 cgagaagatc aactacaagc tcctgcccgg ccccaacaag atgctgccaa aggtgttctt
     4801 ctccaaaaag tggatggcct attacaaccc ttctgaggat atccagaaga tctataagaa
     4861 cggaacattc aagaagggcg atatgttcaa cctgaatgac tgccacaagc tgatcgactt
     4921 ctttaaagac agcatctcca gataccctaa gtggtccaac gcctacgact tcaacttcag
     4981 cgaaacagaa aagtacaaga acatcgccgg cttctacaga gaggtggaag aacaaggcta
     5041 caaggtcagc ttcgagagcg ctagcaagaa ggaggtggac aagctggtgg aggagggaaa
     5101 gctgtacatg ttccagatct acaacaagga tttcagcgac aagagccacg gcacacctaa
     5161 cctgcacacc atgtatttca agctgctgtt tgacgagaac aaccatggcc agatccggct
     5221 gtctggcggc gccgaactgt tcatgcggag agcctctttg aagaaggaag agctggtggt
     5281 gcaccctgcc aatagcccta tcgccaacaa gaacccagat aacccaaaga aaaccaccac
     5341 actgagctac gacgtgtaca aagataagag attcagcgag gatcagtacc agctgcatat
     5401 ccctatcgcc atcaataagt gtcctaaaaa catcttcaag atcaataccg aggtgcgggt
     5461 gttgctgaag cacgacgata acccttacgt gatcggaatc gacaggggcg agagaaacct
     5521 gctgtacatc gtggtggtgg acggcaaggg caatatcgtg gagcaataca gcctgaatga
     5581 gatcatcaac aatttcaacg gcatccgcat aaaaacagat taccactctc tgctggataa
     5641 gaaagagaaa gagcggtttg aggccagaca gaactggacc agcatcgaga atatcaaaga
     5701 gttaaaggcc ggctacatca gccaggtggt gcacaaaatc tgcgagctgg tggaaaagta
     5761 cgacgctgtg atcgcccttg aggatctaaa cagcggcttt aagaacagca gagtgaaggt
     5821 agaaaagcag gtgtatcaga agttcgagaa gatgctcatc gataagctga actacatggt
     5881 ggataagaaa agcaacccct gcgccacagg cggagctctg aagggctacc agatcaccaa
     5941 caaattcgag agctttaaga gcatgtcgac ccagaatggt ttcatctttt acatccccgc
     6001 ctggctcacc tcaaagatcg atcccagcac aggcttcgtc aacctgctga aaaccaagta
     6061 caccagcatc gccgatagca agaagtttat ctcgtccttc gacagaatca tgtacgtgcc
     6121 cgaggaggac ctgttcgagt tcgccctgga ctacaagaac ttctcccgga cagatgccga
     6181 ctacattaag aagtggaagc tgtacagcta cggaaacagg atcagaatct ttagaaaccc
     6241 caaaaagaac aacgtattcg attgggagga agtgtgcctg acctcggcct acaaggagct
     6301 gttcaacaag tacggcatca actaccagca gggcgacatc cgggccctgc tctgtgaaca
     6361 gagcgacaag gctttctaca gcagcttcat ggccctgatg agcctgatgc tgcagatgag
     6421 aaacagcatc acaggaagaa cagacgtgga ctttctgatc tctcctgtga agaacagcga
     6481 cggcatcttc tacgacagca gaaactatga agcccaggag aacgcgatcc tgcctaagaa
     6541 cgcagacgcc aacggcgcct acaatatcgc cagaaaggtg ctgtgggcca ttggccagtt
     6601 caagaaggct gaagatgaga aactggataa agtgaagatc gccatctcca acaaggaatg
     6661 gctggaatac gcccagacca gcgtgaagca caaaaggccg gcggccacga aaaaggccgg
     6721 ccaggcaaaa aagaaaaagg gatcctctgg tggttctccc aagaagaaga ggaaagtcta
     6781 aagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     6841 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     6901 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     6961 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     7021 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     7081 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     7141 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     7201 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     7261 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     7321 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7381 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7441 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7501 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7561 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7621 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7681 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     7741 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7801 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     7861 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7921 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7981 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8041 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8101 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8161 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8221 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8281 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8341 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8401 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8461 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8521 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8581 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8641 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaa
//
