LOCUS       Exported                8125 bp ds-DNA     circular UNA 29-JUL-2025
DEFINITION  obtaining full Cas9 RNP.
ACCESSION   urn.local...8c-jgybxjl
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     regulatory      75..103
                     /standard_name="tac promoter"
                     /label=tac promoter
                     /note="Geneious type: promoter"
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    109..125
                     /standard_name="lac operator"
                     /label=lac operator binding site
                     /bound_moiety="lac repressor encoded by
                     lacI"
                     /note="The lac repressor binds to the lac
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     5'UTR           147..280
                     /gene="cspA"
                     /standard_name="cspA 5'UTR"
                     /label=cspA 5'UTR
                     /note="5'UTR of the E. coli cold shock protein cspA gene 
                     (Mitt a et al., 1997)"
     CDS             281..292
                     /codon_start=1
                     /standard_name="start"
                     /label=start
                     /translation="MKSS"
     CDS             293..310
                     /codon_start=1
                     /standard_name="6xHis"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             317..721
                     /codon_start=1
                     /standard_name="CL7"
                     /label=CL7
                     /translation="SKSNEPGKATGEGKPVNNKWLNNAGKDLGSPVPDRIANKLRDKEF
                     ESFDDFRETFWEEVSKDPELSKQFSRNNNDRMKVGKAPKTRTQDVSGKRTSFELNHQKP
                     IEQNGGVYDMDNISVVTPKRNIDIEGGGGGS"
     CDS             722..745
                     /codon_start=1
                     /standard_name="HRV 3C site"
                     /product="recognition and cleavage site for human
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             755..775
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             785..805
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             833..4516
                     /codon_start=1
                     /standard_name="LbCpf1(wt-9CWF)"
                     /label=LbCpf1(wt-9CWF)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.457"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNVNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLINKLNYMVDKKSNPYATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFANLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     LGDIRVLLCEQSDKAFYSSFMALMTLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAIPNKEWLEYAQTS
                     VKH"
     primer_bind     833..959
                     /standard_name="LbCpf1_BsmBI_F"
                     /label=LbCpf1_BsmBI_F
     primer_bind     833..959
                     /standard_name="oBFC0232"
                     /label=oBFC0232
     primer_bind     833..861
                     /standard_name="oBFC0618"
                     /label=oBFC0618
     primer_bind     complement(1496..1532)
                     /standard_name="oBFC0821"
                     /label=oBFC0821
     primer_bind     1527..1556
                     /standard_name="oBFC0820"
                     /label=oBFC0820
     primer_bind     1548..1578
                     /standard_name="oBFC0262"
                     /label=oBFC0262
     primer_bind     complement(2247..2268)
                     /standard_name="LbCpf1_front_BsmBI_R"
                     /label=LbCpf1_front_BsmBI_R
     primer_bind     2263..2295
                     /standard_name="LbCpf1_mid_BsmBI_F"
                     /label=LbCpf1_mid_BsmBI_F
     misc_feature    complement(2407..2435)
                     /standard_name="PL_Insert_BB_RV "
                     /label=PL_Insert_BB_RV
     misc_feature    2408..2435
                     /standard_name="PL Insert_FW "
                     /label=PL Insert_FW
     primer_bind     complement(3062..3081)
                     /standard_name="oBFC0893"
                     /label=oBFC0893
     primer_bind     3078..3107
                     /standard_name="oBFC0819"
                     /label=oBFC0819
     primer_bind     complement(3128..3168)
                     /standard_name="LbCpf1_mid_BsmBI_R"
                     /label=LbCpf1_mid_BsmBI_R
     primer_bind     complement(3128..3168)
                     /standard_name="oBFC0235"
                     /label=oBFC0235
     primer_bind     3162..3202
                     /standard_name="LbCpf1_mid2_BsmBI_F"
                     /label=LbCpf1_mid2_BsmBI_F
     primer_bind     3162..3202
                     /standard_name="oBFC0236"
                     /label=oBFC0236
     primer_bind     complement(3171..3208)
                     /standard_name="oBFC0818"
                     /label=oBFC0818
     primer_bind     3206..3233
                     /standard_name="oBFC0892"
                     /label=oBFC0892
     misc_feature    complement(3245..3287)
                     /standard_name="PL_Insert_RV"
                     /label=PL_Insert_RV
     misc_feature    3266..3293
                     /standard_name="Lb_FW (CL7_BB_FW)"
                     /label=Lb_FW (CL7_BB_FW)
     primer_bind     complement(3583..3617)
                     /standard_name="LbCpf1_E925A_BsmBI_R"
                     /label=LbCpf1_E925A_BsmBI_R
     primer_bind     complement(3583..3617)
                     /standard_name="LbCpf1_E925Q_BsmBI_R"
                     /label=LbCpf1_E925Q_BsmBI_R
     primer_bind     complement(3583..3617)
                     /standard_name="oBFC0625"
                     /label=oBFC0625
     primer_bind     complement(3583..3617)
                     /standard_name="oBFC0237"
                     /label=oBFC0237
     primer_bind     complement(3583..3617)
                     /standard_name="oBFC0238"
                     /label=oBFC0238
     primer_bind     3610..3643
                     /standard_name="LbCpf1_mid3_BsmBI_F"
                     /label=LbCpf1_mid3_BsmBI_F
     primer_bind     3610..3643
                     /standard_name="oBFC0239"
                     /label=oBFC0239
     primer_bind     complement(3898..3937)
                     /standard_name="LbCpf1_mid3_BsmBI_R"
                     /label=LbCpf1_mid3_BsmBI_R
     primer_bind     complement(3898..3937)
                     /standard_name="oBFC0240"
                     /label=oBFC0240
     primer_bind     complement(3929..3948)
                     /standard_name="oBFC0633"
                     /label=oBFC0633
     primer_bind     3932..3976
                     /standard_name="LbCpf1_end_BsmBI_F"
                     /label=LbCpf1_end_BsmBI_F
     primer_bind     3932..3976
                     /standard_name="oBFC0241"
                     /label=oBFC0241
     primer_bind     3944..3962
                     /standard_name="oBFC0632"
                     /label=oBFC0632
     primer_bind     complement(4429..4453)
                     /standard_name="oBFC0626"
                     /label=oBFC0626
     primer_bind     complement(4490..4516)
                     /standard_name="LbCpf1_BsmBI_R"
                     /label=LbCpf1_BsmBI_R
     primer_bind     complement(4490..4516)
                     /standard_name="oBFC0242"
                     /label=oBFC0242
     misc_feature    4497..4547
                     /standard_name="PL_Insert_BB_FW(CL7_3'end_FW)"
                     /label=PL_Insert_BB_FW(CL7_3'end_FW)
     misc_feature    complement(4497..4538)
                     /standard_name="Lb_PL_Insert_3'endRV(forCL7)"
                     /label=Lb_PL_Insert_3'endRV(forCL7)
     primer_bind     complement(4501..4512)
                     /standard_name="oBFC0771"
                     /label=oBFC0771
     CDS             4526..4545
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /label=SV40 NLS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.23809523809523"
                     /note="/other=CDS"
                     /translation="PKKKRK"
     CDS             4556..4576
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /label=SV40 NLS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="PKKKRKV"
     CDS             4586..4606
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4616..4636
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     3'UTR           4647..4791
                     /gene="cspA"
                     /standard_name="cspA 3'UTR"
                     /label=cspA 3'UTR
                     /note="3'UTR of the E. coli cold shock protein cspA gene 
                     (Mitt a et al., 1997)"
     regulatory      4867..4938
                     /standard_name="rrnB T1 terminator"
                     /label=rrnB T1 terminator
                     /note="Geneious type: terminator"
                     /note="transcription terminator T1 from the E. coli
                     rrnB gene"
     regulatory      5044..5148
                     /gene="bla"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
                     /note="Geneious type: promoter"
     CDS             5149..6009
                     /codon_start=1
                     /gene="bla"
                     /standard_name="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6180..6768
     CDS             complement(6940..8022)
                     /codon_start=1
                     /gene="lacI"
                     /standard_name="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     regulatory      complement(8023..8100)
                     /gene="lacI"
                     /standard_name="lacI promoter"
                     /label=lacI promoter
                     /note="Geneious type: promoter"
ORIGIN
        1 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
       61 aaggaatggt gtggttgaca attaatcatc ggctcgtata atgtgtaatt gtgagcggat
      121 aacaatttga tgtgctagcg catatccagt gtagtaaggc aagtcccttc aagagttatc
      181 gttgataccc ctcgtagtgc acattccttt aacgcttcaa aatctgtaaa gcacgccata
      241 tcgccgaaag gcacacttaa ttattaagag gtaatacacc atgaaatctt ctcaccatca
      301 ccatcaccat ggttctagca aaagcaatga accgggtaaa gcaaccggtg aaggtaaacc
      361 ggttaataac aaatggctga acaatgccgg taaagatctg ggtagtccgg ttccggatcg
      421 tattgcaaat aaactgcgtg ataaagaatt cgagagcttc gatgattttc gtgaaacctt
      481 ttgggaagaa gttagcaaag atcctgaact gagcaaacag tttagccgca ataacaatga
      541 tcgtatgaaa gttggtaaag caccgaaaac acgtacccag gatgttagcg gtaaacgtac
      601 ctcatttgaa ctgaatcatc agaaaccgat tgaacagaat ggtggcgttt atgatatgga
      661 taacattagc gttgttaccc cgaaacgcaa cattgatatt gaaggcggtg gtggtggttc
      721 tttggaggtt ttgttccagg gtccaaacgc gacaccaaag aaaaagcgta aagtcggggg
      781 atctccgaaa aagaaacgga aagtaggtat ccacggagtc ccagcagcta ccatgagcaa
      841 actggagaag ttcacgaact gttattctct gtcgaaaacc ttacgtttta aggccattcc
      901 ggtaggtaaa acgcaagaaa atatcgacaa taaacgtcta ctggttgaag atgaaaagcg
      961 tgccgaagat tataaaggtg ttaaaaagct gcttgatcgc tattacctgt cttttatcaa
     1021 cgatgttctg cattccatca aattgaaaaa cttgaataat tacatctcgt tatttcgcaa
     1081 aaaaacccgt accgaaaaag aaaacaagga gctggaaaac cttgaaatta atctgcgtaa
     1141 agaaatcgcg aaagcattta aaggtaatga aggatataaa tctctgttta aaaaagacat
     1201 tattgaaacc atcctgcctg agttcttgga tgataaagat gaaattgccc tggttaatag
     1261 ctttaatggc tttacgactg catttaccgg cttttttgat aaccgtgaaa acatgttcag
     1321 cgaagaagcg aaatcgactt ccattgcctt ccgttgcatt aatgaaaacc tgacccgcta
     1381 catttctaat atggacatct ttgaaaaagt tgatgccatt ttcgacaaac atgaagttca
     1441 agaaatcaaa gaaaagattt taaactccga ttatgatgtt gaagatttct ttgagggaga
     1501 atttttcaat tttgttctca cccaagaggg tattgatgta tataacgcca ttatcggagg
     1561 atttgttacg gaatcaggcg aaaagattaa aggactgaat gaatacatta atttatacaa
     1621 tcagaaaacc aaacagaaat tgccaaaatt caaaccactg tataaacagg tattaagcga
     1681 ccgtgaatcc ttgtcctttt atggcgaagg ctatacaagc gatgaagagg tgctcgaagt
     1741 attccgtaat actttgaaca aaaactcgga aatcttctcc agcattaaaa aactggaaaa
     1801 attgtttaaa aatttcgatg aatatagtag cgcaggcatc ttcgtcaaaa atggccctgc
     1861 gatttcaacc atttctaaag acattttcgg tgaatggaac gtgatccgcg ataaatggaa
     1921 cgcggagtat gacgacattc atctgaaaaa aaaagccgtt gttaccgaaa agtatgaaga
     1981 tgaccgccgc aagagcttta aaaaaattgg tagtttctca ctggaacagt tacaagaata
     2041 tgcagacgcc gatctgtcgg ttgtggaaaa gttaaaagag attattatcc agaaagtcga
     2101 tgaaatttat aaagtgtacg gctccagcga aaagctcttt gatgccgact ttgttttaga
     2161 aaaatcgctg aaaaaaaacg atgccgtggt ggccatcatg aaagatctgt tggattcggt
     2221 caaatcgttc gaaaactata tcaaggcgtt ctttggcgaa gggaaagaaa ccaatcgtga
     2281 cgaatcgttc tatggcgatt ttgtgttggc gtatgatatt cttctgaaag tagatcacat
     2341 ctatgatgct atccgcaact acgtgaccca gaaaccgtat tcgaaggata aatttaagct
     2401 gtattttcaa aaccctcagt ttatgggtgg ctgggataaa gataaagaaa ccgactaccg
     2461 cgcgacgatc ttgcgctatg gctctaagta ctatttagcc atcatggata aaaagtatgc
     2521 aaaatgcctc cagaaaatcg ataaggatga tgtcaacggg aactacgaaa aaatcaacta
     2581 caaactcttg cctggcccga ataaaatgct gccgaaagtc tttttttcca aaaaatggat
     2641 ggcctattat aacccttccg aagatattca gaagatttac aagaacggta cctttaaaaa
     2701 aggtgacatg tttaatctga atgactgtca caagcttatt gatttcttca aagatagtat
     2761 ttctcgctac cctaaatggt caaacgcgta cgattttaat ttctcagaaa cagagaaata
     2821 caaagatatt gcggggtttt atcgcgaagt cgaagaacaa gggtataagg tgtcgttcga
     2881 atcggcttcc aaaaaggaag tggataaact tgttgaagaa ggcaaactct atatgttcca
     2941 gatctacaac aaagatttct ccgacaagag ccatggcacg ccgaacttac ataccatgta
     3001 ctttaaatta ctgttcgatg aaaataatca tggtcagatt cgcttgagcg gtggcgccga
     3061 actgtttatg cgccgcgcat cattgaaaaa ggaagaactt gttgtgcacc cagcaaacag
     3121 tccgattgca aacaaaaatc cggataaccc gaagaaaact accaccttaa gctatgatgt
     3181 atataaagat aaacgtttta gcgaagatca gtatgaactc cacattccga tcgcgattaa
     3241 caaatgtcct aagaacatct ttaaaatcaa taccgaggtt cgtgtactgc tcaaacacga
     3301 cgacaacccc tatgttattg gcattgaccg cggcgaacgc aacctgctgt atattgtcgt
     3361 tgtggatggc aaaggtaaca ttgttgaaca gtatagcctt aatgaaatca tcaataatgt
     3421 caacggcatt cgtattaaaa cggactatca tagcctgctg gacaaaaaag agaaagaacg
     3481 cttcgaagcc cgtcagaatt ggacctctat cgaaaatatc aaggaactga aagctgggta
     3541 catttctcaa gtggtccaca agatttgcga actggtggaa aagtatgacg cggtgatcgc
     3601 actggaggat ctgaattcgg gctttaaaaa tagccgtgtg aaggtggaga aacaggttta
     3661 ccaaaagttc gaaaaaatgc tgattaacaa acttaattac atggtggaca aaaaatctaa
     3721 tccatatgcg accggcgggg ctctcaaagg ctatcagatt acgaataaat tcgaaagctt
     3781 caaatccatg tctacccaga acggatttat cttttacatc cccgcctggc tgacttccaa
     3841 aattgatccg agtaccggtt ttgctaatct gctgaaaacc aaatatacgt cgatcgccga
     3901 ttcaaaaaaa ttcattagca gcttcgaccg tattatgtac gtcccggaag aagatctgtt
     3961 cgaattcgcc cttgactata aaaatttctc gcgtacggat gccgattata tcaaaaaatg
     4021 gaaactgtat agctacggca accgcatccg catttttcgc aacccaaaga agaacaacgt
     4081 atttgactgg gaggaagttt gcttgacgag cgcttacaaa gagctcttta acaaatatgg
     4141 gatcaattat cagctagggg acatccgtgt gttgctgtgt gagcagtcag ataaagcgtt
     4201 ctacagctct ttcatggctt taatgacgct gatgttgcag atgcgcaatt cgatcactgg
     4261 ccgcaccgat gtcgatttcc tgattagccc ggtcaaaaat agcgatggca ttttttacga
     4321 ttcacgtaac tatgaagccc aggaaaacgc tattctgccg aagaacgctg atgcgaacgg
     4381 cgcgtacaat attgcccgta aggtattgtg ggcaattggt cagttcaaga aagcggaaga
     4441 tgagaagttg gataaggtca aaattgcgat cccgaataaa gaatggctgg aatatgcgca
     4501 gaccagcgtg aaacatggcg gatcaccgaa gaagaaacgc aaagtcggag gtagccctaa
     4561 gaagaagcgt aaggttgggg ggtcgccaaa gaagaaacgt aaagttgggg gctcgccgaa
     4621 aaagaaacgc aaggtgtaat aggtaatctc tgcttaaaag cacagaatct aagatccctg
     4681 ccatttggcg gggatttttt tatttgtttt caggaaataa ataatcgatc gcgtaataaa
     4741 atctattatt atttttgtga agaataaatt tgggtgcaat gagaatgcgc aggccctttc
     4801 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgtcgagt aaggatctcc
     4861 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     4921 ttgtcggtga acgctctcgt cgacactatg gttgctttga cgtatgcggt gtgaaatacc
     4981 gcacagatgc gtaaggagaa aataccgcat caggcgtcag gtggcacttt tcggggaaat
     5041 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5101 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5161 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5221 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5281 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5341 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5401 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5461 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5521 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5581 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5641 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5701 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5761 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5821 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5881 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5941 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6001 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6061 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6121 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6181 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6241 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6301 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6361 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6421 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6481 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6541 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6601 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6661 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6721 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6781 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatagt catgccccgc
     6841 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     6901 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6961 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7021 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     7081 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     7141 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     7201 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     7261 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     7321 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     7381 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     7441 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     7501 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     7561 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     7621 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     7681 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     7741 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     7801 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     7861 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     7921 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     7981 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     8041 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
     8101 cgggatctcg acgctctccc ttatg
//
