LOCUS       Exported                9739 bp ds-DNA     circular UNA 02-JAN-2025
DEFINITION  Generated by In-Fusion Cloning (Kleinstiver dLbBE1.4 Plasmid 
            (digested by SacI, NotI) Fragment 2, dLbCas12a_RVQ - RVQ gBlock1, 
            dLbCas12a_RVQ-PL4a - RVQ-PL4a gBlock2, dLbCas12a - WT gBlock3).
ACCESSION   urn.local...s9-ih3tj7x
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..1431,6177..9739)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1412..2123
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5805..6200
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          163..1179
                     /standard_name="chimeric intron"
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1103..1125
                     /standard_name="pCAGGS-5"
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1187..1206
                     /standard_name="pCAG-F"
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG
                     plasmids,
                     forward primer"
     regulatory      1272..1281
                     /regulatory_class="other"
                     /standard_name="Kozak sequence"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong
                     initiation
                     of translation (Kozak, 1987)"
     CDS             1284..1304
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40
                     (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1314..1334
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40
                     (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1344..2027
                     /codon_start=2
                     /standard_name="APOBEC-1"
                     /product="cytidine deaminase (C to U editing enzyme)
                     from
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="AQRLAQWLWTPH*DGGSSPMSLRYSSIRESSARRPACFTKLIGGA
                     GTPFGDIHHRTLTSTSKSTSSRSSRQKDISVRTQGAALPGFSAGAHAANVVGPSLNSCQ
                     GIPTSLCLFTSQGCTTTLTPAIDKACGI*SLQV*LSKL*LSRSQDTAGETL*IIARVMK
                     PTGLGIPICGYDCTFLNCTASYWACLLVSTF*EGSSHS*HSLPSLFSLVITSDCPHTFS
                     GPPG"
     misc_feature    1412..3077
                     /standard_name="dLbCas12a_RVQ - RVQ gBlock1 (concatenated
                     seque"
                     /label=dLbCas12a_RVQ - RVQ gBlock1 (concatenated
                     seque
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1412..3077
                     /standard_name="RVQ gBlock1"
                     /label=RVQ gBlock1
                     /note="Geneious type: gBlock"
                     /note="/created_by=kevinwasko"
                     /note="/modified_by=kevinwasko"
     misc_feature    1412..2123
                     /standard_name="ATG"
                     /label=ATG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=ATG"
     misc_feature    1412..1431
                     /standard_name="Ligation"
                     /label=Ligation
                     /note="Geneious type: ligation"
     primer_bind     2028..2053
                     /standard_name="HS_1379"
                     /label=HS_1379
     CDS             2124..5804
                     /codon_start=1
                     /standard_name="dLbCas12a_opt"
                     /label=dLbCas12a_opt
                     /note="/created_by=kevinwasko"
                     /note="/modified_by=kevinwasko"
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNRFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTVYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWGKDKETDYRATILRYGFKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKNIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYQLHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     misc_feature    2556..2558
                     /standard_name="G146R"
                     /label=G146R
                     /note="Geneious type: RVQ"
                     /note="/created_by=kevinwasko"
     misc_feature    2556
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    2558
                     /standard_name="C"
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    2664..2666
                     /standard_name="R182V"
                     /label=R182V
                     /note="Geneious type: RVQ"
                     /note="/created_by=kevinwasko"
     misc_feature    2664..2665
                     /standard_name="CG"
                     /label=CG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=CG"
     misc_feature    3058..4684
                     /standard_name="dLbCas12a_RVQ-PL4a - RVQ-PL4a gBlock2
                     (concaten"
                     /label=dLbCas12a_RVQ-PL4a - RVQ-PL4a gBlock2
                     (concaten
                     /note="Geneious type: Concatenated sequence"
     misc_feature    3058..4684
                     /standard_name="RVQ-PL4a gBlock2"
                     /label=RVQ-PL4a gBlock2
                     /note="Geneious type: gBlock"
                     /note="/created_by=kevinwasko"
                     /note="/modified_by=kevinwasko"
     misc_feature    3058..3077
                     /standard_name="RVQ/WT gBlock2"
                     /label=RVQ/WT gBlock2
                     /note="Geneious type: gBlock"
                     /note="/created_by=kevinwasko"
                     /note="/modified_by=kevinwasko"
     misc_feature    3058..3077
                     /standard_name="Ligation"
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    3058..3077
                     /standard_name="WT gBlock1"
                     /label=WT gBlock1
                     /note="Geneious type: gBlock"
                     /note="/created_by=kevinwasko"
     misc_feature    3723..3725
                     /standard_name="D535G"
                     /label=D535G
                     /note="Geneious type: PL4a"
                     /note="/created_by=kevinwasko"
     misc_feature    3723..3725
                     /standard_name="D535G"
                     /label=D535G
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    3724
                     /standard_name="A"
                     /label=A
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=A"
     misc_feature    3732..3734
                     /standard_name="K538N"
                     /label=K538N
                     /note="Geneious type: PL2d"
                     /note="/created_by=kevinwasko"
     misc_feature    3765..3767
                     /standard_name="Y549C"
                     /label=Y549C
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    3771..3773
                     /standard_name="S551F"
                     /label=S551F
                     /note="Geneious type: PL4a"
                     /note="/created_by=kevinwasko"
     misc_feature    3771..3773
                     /standard_name="S551F"
                     /label=S551F
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    3772
                     /standard_name="C"
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
     misc_feature    3777..3779
                     /standard_name="Y553F"
                     /label=Y553F
                     /note="Geneious type: PL2d"
                     /note="/created_by=kevinwasko"
     misc_feature    4113..4115
                     /standard_name="D665N"
                     /label=D665N
                     /note="Geneious type: PL4a"
                     /note="/created_by=kevinwasko"
     misc_feature    4113..4115
                     /standard_name="D665N"
                     /label=D665N
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    4113
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    4191..4193
                     /standard_name="D691G"
                     /label=D691G
                     /note="Geneious type: PL2d"
                     /note="/created_by=kevinwasko"
     misc_feature    4347..4349
                     /standard_name="E743K"
                     /label=E743K
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    4374..4376
                     /standard_name="K752R"
                     /label=K752R
                     /note="Geneious type: PL2d"
                     /note="/created_by=kevinwasko"
     misc_feature    4503..4505
                     /standard_name="E795Q"
                     /label=E795Q
                     /note="Geneious type: RVQ"
                     /note="/created_by=kevinwasko"
     misc_feature    4503
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    4515..4517
                     /standard_name="P799R"
                     /label=P799R
                     /note="Geneious type: PL2d"
                     /note="/created_by=kevinwasko"
     misc_feature    4614..4616
                     /standard_name="D832A"
                     /label=D832A
                     /note="Geneious type: Catalytic Inactivation"
                     /note="/created_by=kevinwasko"
     misc_feature    4615
                     /standard_name="A"
                     /label=A
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=A"
     misc_feature    4665..6200
                     /standard_name="WT gBlock3"
                     /label=WT gBlock3
                     /note="Geneious type: gBlock"
                     /note="/created_by=kevinwasko"
     misc_feature    4665..6200
                     /standard_name="dLbCas12a - WT gBlock3 (concatenated
                     sequence 4)"
                     /label=dLbCas12a - WT gBlock3 (concatenated
                     sequence 4)
                     /note="Geneious type: Concatenated sequence"
     misc_feature    4665..4684
                     /standard_name="Ligation"
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    4665..4684
                     /standard_name="WT gBlock3"
                     /label=WT gBlock3
                     /note="Geneious type: gBlock"
                     /note="/created_by=kevinwasko"
     misc_feature    4665..4684
                     /standard_name="WTgBlock2"
                     /label=WTgBlock2
                     /note="Geneious type: gBlock"
                     /note="/created_by=kevinwasko"
     misc_feature    4707..4709
                     /standard_name="F863V"
                     /label=F863V
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    4974..4976
                     /standard_name="D952N"
                     /label=D952N
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    5013..5015
                     /standard_name="C965Y"
                     /label=C965Y
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    5151..5153
                     /standard_name="V1011A"
                     /label=V1011A
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    5442..5444
                     /standard_name="Q1108L"
                     /label=Q1108L
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    5457..5459
                     /standard_name="A1113V"
                     /label=A1113V
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    5514..5516
                     /standard_name="S1132T"
                     /label=S1132T
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     misc_feature    5760..5762
                     /standard_name="S1214P"
                     /label=S1214P
                     /note="Geneious type: PL34a"
                     /note="/created_by=kevinwasko"
     primer_bind     complement(5785..5804)
                     /standard_name="HS_1380"
                     /label=HS_1380
     CDS             5805..5852
                     /codon_start=1
                     /standard_name="nucleoplasmin NLS"
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5889..6137
                     /codon_start=1
                     /standard_name="UGI"
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6150..6170
                     /codon_start=1
                     /standard_name="SV40 NLS"
                     /product="nuclear localization signal of SV40
                     (simian
                     virus 40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    join(6177..9739,1..1431)
                     /standard_name="Kleinstiver dLbBE1.4 Plasmid (digested by
                     SacI,"
                     /label=Kleinstiver dLbBE1.4 Plasmid (digested by
                     SacI,
                     /note="Geneious type: Concatenated sequence"
     misc_feature    6177..6200
                     /standard_name="Ligation"
                     /label=Ligation
                     /note="Geneious type: ligation"
     primer_bind     complement(6260..6279)
                     /standard_name="Bglob-pA-R"
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     misc_feature    6325..6380
                     /standard_name="beta-globin poly(A) signal"
                     /label=beta-globin poly(A) signal
                     /note="Geneious type: polyA_signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6379..6398)
                     /standard_name="rbglobpA-R"
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer.
                     Also
                     called rb-glob-pA-term-R"
     primer_bind     complement(6741..6757)
                     /standard_name="M13 rev"
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6741..6757)
                     /standard_name="M13 Reverse"
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6754..6776)
                     /standard_name="M13/pUC Reverse"
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6765..6781
                     /standard_name="lac operator"
                     /label=lac operator binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator
                     to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(6789..6819)
                     /standard_name="lac promoter"
                     /label=lac promoter
                     /note="Geneious type: promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6834..6855
                     /standard_name="CAP binding site"
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presence
                     of cAMP."
     regulatory      6914..7109
                     /standard_name="SV40 promoter"
                     /label=SV40 promoter
                     /note="Geneious type: promoter"
                     /note="SV40 early promoter"
     rep_origin      6960..7095
     misc_feature    7115..7249
                     /standard_name="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="Geneious type: polyA_signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7152..7171)
                     /standard_name="SV40pA-R"
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7206..7225
                     /standard_name="EBV-rev"
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7317..7334)
                     /standard_name="L4440"
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7488..8076)
                     /direction=LEFT
     primer_bind     complement(7568..7587)
                     /standard_name="pBR322ori-F"
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8247..9107)
                     /codon_start=1
                     /gene="bla"
                     /standard_name="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin,
                     carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8870..8889
                     /standard_name="Amp-R"
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     regulatory      complement(9108..9212)
                     /gene="bla"
                     /standard_name="AmpR promoter"
                     /label=AmpR promoter
                     /note="Geneious type: promoter"
     regulatory      9243..9622
                     /standard_name="CMV enhancer"
                     /label=CMV enhancer
                     /note="Geneious type: enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggcgc gccmgggggg gggggggggg gggggggggg gggggggggg rggggggggg
       61 gggggggggg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      121 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      181 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      241 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
      301 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
      361 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
      421 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
      481 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
      541 ggggggggct gcgrggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
      601 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
      661 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
      721 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc kcgggccggg
      781 gagggctcgg gggrggggcg cggcggcccc cggrgcgccg gcggctgtcg aggcgcggcg
      841 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
      901 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
      961 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     1021 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     1081 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1141 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1201 attgtgctgt ctcatcattt tggcaaagaa ttctgcagtc gacggtaccg cgggcccggg
     1261 atccaccggt cgccaccatg ggcccaaaga aaaagaggaa agtcggcagt ggacctaaaa
     1321 agaaacgaaa ggttgggtca ggtagctcag agactggccc agtggctgtg gaccccacat
     1381 tgagacggcg gatcgagccc catgagtttg aggtattctt cgatccgaga gagctccgca
     1441 aggagacctg cctgctttac gaaattaatt gggggggccg gcactccatt tggcgacata
     1501 catcacagaa cactaacaag cacgtcgaag tcaacttcat cgagaagttc acgacagaaa
     1561 gatatttctg tccgaacaca aggtgcagca ttacctggtt tctcagctgg agcccatgcg
     1621 gcgaatgtag tagggccatc actgaattcc tgtcaaggta tccccacgtc actctgttta
     1681 tttacatcgc aaggctgtac caccacgctg acccccgcaa tcgacaaggc ctgcgggatt
     1741 tgatctcttc aggtgtgact atccaaatta tgactgagca ggagtcagga tactgctgga
     1801 gaaactttgt gaattatagc ccgagtaatg aagcccactg gcctaggtat ccccatctgt
     1861 gggtacgact gtacgttctt gaactgtact gcatcatact gggcctgcct ccttgtctca
     1921 acattctgag aaggaagcag ccacagctga cattctttac catcgctctt cagtcttgtc
     1981 attaccagcg actgccccca cacattctct gggccaccgg gttgaaatct ggtggttctt
     2041 ctggtggttc tagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagtt
     2101 ccggagggag tagcggcggg tctagcaagc tggaaaaatt taccaactgt tacagcctga
     2161 gcaaaaccct gaggttcaag gccatccccg tgggcaagac ccaagagaac atcgataaca
     2221 agagactgct ggtcgaggat gagaagcgtg cagaggacta caagggcgtg aagaagctcc
     2281 tggatcggta ctacctgagt ttcatcaacg acgtgctgca cagcatcaag ctgaagaacc
     2341 tgaacaacta catcagcctg tttagaaaga aaaccagaac cgaaaaagaa aataaggagc
     2401 tggaaaatct ggaaatcaac ctgagaaagg aaatcgctaa ggcctttaag ggaaacgaag
     2461 gatacaaatc cctgtttaag aaagacatca tcgagacaat tctgccagag ttcctggacg
     2521 acaaagacga gatagctctt gtgaacagct tcaacaggtt cacgaccgcc ttcaccggat
     2581 tcttcgacaa cagggaaaac atgttcagtg aggaagccaa gtctaccagc atcgctttcc
     2641 ggtgcatcaa tgagaacctg accgtgtata tctctaatat ggatatcttt gaaaaagtgg
     2701 acgctatttt cgacaagcac gaggtgcagg agatcaagga gaagatcctg aacagcgact
     2761 acgacgtgga ggacttcttc gagggcgaat ttttcaactt cgtgctgaca caagagggca
     2821 tcgacgttta caacgccatc ataggcggct tcgtgaccga gtccggcgaa aagatcaagg
     2881 gccttaacga gtacatcaac ctgtataacc agaaaaccaa gcagaagctg cctaagttca
     2941 agcctcttta taagcaggtc ctgagcgacc gggaaagcct gagcttctac ggcgagggct
     3001 acacatctga cgaggaagtg ctggaggtct ttcggaatac cctgaacaag aactctgaga
     3061 tcttcagctc tattaagaaa ctggaaaagc tgttcaagaa tttcgacgag tatagtagcg
     3121 ccgggatctt cgtgaagaac ggccctgcca ttagcaccat cagcaaggac atcttcggcg
     3181 aatggaacgt tatccgcgat aagtggaacg ccgagtacga tgacatccac ctgaaaaaga
     3241 aggccgtggt gaccgagaag tacgaagatg acagaagaaa gagcttcaaa aagatcggct
     3301 catttagcct ggaacagctg caggagtacg ccgacgccga cctgtccgtg gtcgaaaagc
     3361 tgaaggaaat tatcatccaa aaagtggacg agatctataa ggtgtacggg agctctgaga
     3421 agctgttcga cgccgacttt gtgctggaaa agtcactgaa aaagaacgac gctgtggtcg
     3481 ccatcatgaa ggatctgctg gactccgtga agagcttcga gaactacatc aaggccttct
     3541 tcggcgaagg caaggagacc aaccgggacg agtcttttta cggcgatttc gtgctggcct
     3601 acgacatcct gctgaaggtg gaccacatct acgacgccat cagaaactac gtgacccaga
     3661 agccctacag caaagacaag tttaagctgt acttccagaa tcctcagttc atgggaggat
     3721 ggggcaaaga caaggaaacc gactaccggg ctacaattct gagatacggc tttaagtact
     3781 accttgcaat catggacaaa aagtatgcca agtgcctgca gaaaattgat aaggatgacg
     3841 tgaacggcaa ctacgagaag atcaactaca agctcctgcc cggccccaac aagatgctgc
     3901 caaaggtgtt cttctccaaa aagtggatgg cctattacaa cccttctgag gatatccaga
     3961 agatctataa gaacggaaca ttcaagaagg gcgatatgtt caacctgaat gactgccaca
     4021 agctgatcga cttctttaaa gacagcatct ccagataccc taagtggtcc aacgcctacg
     4081 acttcaactt cagcgaaaca gaaaagtaca agaacatcgc cggcttctac agagaggtgg
     4141 aagaacaagg ctacaaggtc agcttcgaga gcgctagcaa gaaggaggtg gacaagctgg
     4201 tggaggaggg aaagctgtac atgttccaga tctacaacaa ggatttcagc gacaagagcc
     4261 acggcacacc taacctgcac accatgtatt tcaagctgct gtttgacgag aacaaccatg
     4321 gccagatccg gctgtctggc ggcgccgaac tgttcatgcg gagagcctct ttgaagaagg
     4381 aagagctggt ggtgcaccct gccaatagcc ctatcgccaa caagaaccca gataacccaa
     4441 agaaaaccac cacactgagc tacgacgtgt acaaagataa gagattcagc gaggatcagt
     4501 accagctgca tatccctatc gccatcaata agtgtcctaa aaacatcttc aagatcaata
     4561 ccgaggtgcg ggtgttgctg aagcacgacg ataaccctta cgtgatcgga atcgccaggg
     4621 gcgagagaaa cctgctgtac atcgtggtgg tggacggcaa gggcaatatc gtggagcaat
     4681 acagcctgaa tgagatcatc aacaatttca acggcatccg cataaaaaca gattaccact
     4741 ctctgctgga taagaaagag aaagagcggt ttgaggccag acagaactgg accagcatcg
     4801 agaatatcaa agagttaaag gccggctaca tcagccaggt ggtgcacaaa atctgcgagc
     4861 tggtggaaaa gtacgacgct gtgatcgccc ttgaggatct aaacagcggc tttaagaaca
     4921 gcagagtgaa ggtagaaaag caggtgtatc agaagttcga gaagatgctc atcgataagc
     4981 tgaactacat ggtggataag aaaagcaacc cctgcgccac aggcggagct ctgaagggct
     5041 accagatcac caacaaattc gagagcttta agagcatgtc gacccagaat ggtttcatct
     5101 tttacatccc cgcctggctc acctcaaaga tcgatcccag cacaggcttc gtcaacctgc
     5161 tgaaaaccaa gtacaccagc atcgccgata gcaagaagtt tatctcgtcc ttcgacagaa
     5221 tcatgtacgt gcccgaggag gacctgttcg agttcgccct ggactacaag aacttctccc
     5281 ggacagatgc cgactacatt aagaagtgga agctgtacag ctacggaaac aggatcagaa
     5341 tctttagaaa ccccaaaaag aacaacgtat tcgattggga ggaagtgtgc ctgacctcgg
     5401 cctacaagga gctgttcaac aagtacggca tcaactacca gcagggcgac atccgggccc
     5461 tgctctgtga acagagcgac aaggctttct acagcagctt catggccctg atgagcctga
     5521 tgctgcagat gagaaacagc atcacaggaa gaacagacgt ggactttctg atctctcctg
     5581 tgaagaacag cgacggcatc ttctacgaca gcagaaacta tgaagcccag gagaacgcga
     5641 tcctgcctaa gaacgcagac gccaacggcg cctacaatat cgccagaaag gtgctgtggg
     5701 ccattggcca gttcaagaag gctgaagatg agaaactgga taaagtgaag atcgccatct
     5761 ccaacaagga atggctggaa tacgcccaga ccagcgtgaa gcacaaaagg ccggcggcca
     5821 cgaaaaaggc cggccaggca aaaaagaaaa agggatcctc tggtggttct ggaggatctg
     5881 gtggttctac taatctgtca gatattattg aaaaggagac cggtaagcaa ctggttatcc
     5941 aggaatccat cctcatgctc ccagaggagg tggaagaagt cattgggaac aagccggaaa
     6001 gcgatatact cgtgcacacc gcctacgacg agagcaccga cgagaatgtc atgcttctga
     6061 ctagcgacgc ccctgaatac aagccttggg ctctggtcat acaggatagc aacggtgaga
     6121 acaagattaa gatgctctct ggtggttctc ccaagaagaa gaggaaagtc taaagcggcc
     6181 gcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     6241 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     6301 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     6361 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     6421 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     6481 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     6541 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     6601 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     6661 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     6721 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6781 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6841 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6901 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6961 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7021 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7081 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
     7141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
     7261 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7381 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7681 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7801 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7921 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8161 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8221 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8281 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8341 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8401 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8461 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8521 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8581 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8641 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8701 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8761 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8821 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8881 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8941 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9001 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9061 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
     9241 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     9301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     9361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     9421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     9481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     9541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     9601 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     9661 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     9721 gatgggggcg ggggggggg
//
