LOCUS       pAttBF3Neo__5289        4742 bp    DNA        circular     30-JUL-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     vectorA PCR from 8 (BsaI) to 1553 (BsaI) 
COMMENT     vector B from 8 (BsaI) to 1356 (BsaI) 
COMMENT     attB PCR from 38 (BsaI) to 161 (BsaI) 
COMMENT     PCR pAttPF3Neo [4921 7673 7674 from 12 (BsaI) to 1601 (BsaI)
            (rev-com)
COMMENT     polylinker PCR from 8 (BsaI) to 140 (BsaI) 
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(3772..3792)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      3976..4282
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3889..3957
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1..660
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1845..1864
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      758..1445
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1779..1801
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    1865..1971
                     /locus_tag="attB region"
                     /label="attB region"
                     /ApEinfo_label="attB region"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1865..1928
                     /locus_tag="attB phiC31"
                     /label="attB phiC31"
                     /ApEinfo_label="attB phiC31"
                     /ApEinfo_fwdcolor="#929000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1895..1896
                     /locus_tag="recombination site"
                     /label="recombination site"
                     /ApEinfo_label="recombination site"
                     /ApEinfo_fwdcolor="#ff9300"
                     /ApEinfo_revcolor="#ff9300"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1976..2009)
                     /locus_tag="lox2272"
                     /label="lox2272"
                     /ApEinfo_label="lox2272"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(3181..3542)
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2385..3179)
                     /locus_tag="NeoR"
                     /label="NeoR"
                     /ApEinfo_label="NeoR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 1 offset 0"
     3'UTR           complement(2032..2383)
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3563..3596)
                     /locus_tag="lox2272(1)"
                     /label="lox2272(1)"
                     /ApEinfo_label="lox2272"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3637..3643
                     /locus_tag="< SapI"
                     /label="< SapI"
                     /ApEinfo_label="< SapI"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3701..3734)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3677..3683
                     /locus_tag="SapI >"
                     /label="SapI >"
                     /ApEinfo_label="SapI >"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgTGGAt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaatgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1561 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aattcgccca atacgcaaac
     1621 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1681 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1741 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1801 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     1861 gggaAAGCCG CGGTGCGGGT GCCAGGGCGT GCCCTTGGGC TCCCCGGGCG CGTACTCCAC
     1921 CTCACCCATC TGGTCCATCA TGATGAACGG GTCGAGGTGG CGGTAGTTGA TacaaATAAC
     1981 TTCGTATAAA GTATCCTATA CGAAGTTATc ccagttgtcc tcgaacaGGT CTAAAAATTG
     2041 CGGTCATAAA CTGAAACGTA ACATATGATA AGGTATTTTG TGTGCGGTTT TTTCTATGAT
     2101 GTTTCAAAAA AATTTATCAG AAGTAAAAAA CATAAGAAGT GTACACAGAA CATTGTGTAG
     2161 ATTTTGGGAT AGAATTATTA TTAATGGGGG GAGGGAGCAC AATTTTTTTC GACTTTTTAT
     2221 TACGAATTAA TTCTATTTTT GAATCTACAC AATTTCATTG TTAGAGGTGA CTTAAAAGAA
     2281 GCTAAAAAAC AAAGAAATTA AGAGAAGTTT TTTGATAATA ACAAAAATAG GGGGTGGGAG
     2341 CACAGGGAGA AAGAGCATGT AGGGATGTTG AAGAGTAATT GGACTCAGAA GAACTCGTCA
     2401 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG
     2461 AAGCGGTCAG CCCATTCGCC GCCAAGCTCC TCAGCAATAT CACGGGTAGC CAACGCTATG
     2521 TCCTGATAGC GGTCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA
     2581 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG
     2641 TCGGGCATGC GCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GTGCGAGCCC CTGATGTTCT
     2701 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG
     2761 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC
     2821 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC
     2881 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC
     2941 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCCTGC
     3001 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT
     3061 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG
     3121 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATA
     3181 TTACCTTAAA ATTCAAAAAT TAATTTCAGA TCAGTCATAA CCAAGAAAAC AAAATAAACG
     3241 TTAGGACAGA AAATTCAAAT TAATTAAAAA TTATGCTGAA TAAGAAAATT ACATGCAAAG
     3301 CCTTATTTTC CTCATAGAAA CAGTTGAATA ACATATTTTT TAATTGAAAA CATAGCAAAA
     3361 TTCGTGGTAA AATTTGTCAA AACCTTGGAA AAAATCGAAA AAAGAACATT TCCCGATGCA
     3421 TTTTCCTATT TCTCGTTGCC GACAACGTAT AAACGTTGAA TACTCAGTCA ACGAATTTGG
     3481 CTGCTGGCTT GGACTCTCGC ACGGAAGAGA CGCGTGTGTA GATTTACGAC GAAAGCAAAA
     3541 ATgatcccgc tttccagtcg ggATAACTTC GTATAAAGta tcCTATACGA AGTTATaagc
     3601 tggagctcca ccgcggtggc ggccgctCTA GATGGCGAAG AGCccatgga tccactagta
     3661 acatatgtgc aggcctGCTC TTCGGTAagc ttatcgatac GAAGTTCCTA TACtatttga
     3721 aGAATAGGAA CTTCcgtcga cccgttaacc gggggcccgg tacccaattc gccctatagt
     3781 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3841 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3901 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     3961 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4021 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4081 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4141 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4201 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4261 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4321 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4381 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4441 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4501 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4561 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4621 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4681 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4741 tg     
//
