LOCUS       pHygGP6__4575_          6562 bp    DNA        circular     31-JUL-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Ligation reaction:
COMMENT     pHygG6 [4441] from 3491 (DraII) to 2535 (SpeI)
COMMENT     pHygGP7 [4484] from 4879 (SpeI) to 6414 (DraII)
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(4459..5118)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(5579..5885)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     90..123
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5914..5947
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(160..462,511..585,637..756,808..1020)
                     /locus_tag="GFP-C1"
                     /label="GFP-C1"
                     /ApEinfo_label="GFP-C1"
                     /ApEinfo_fwdcolor="#00ff33"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    5999..6219
                     /locus_tag="full attP "
                     /label="full attP "
                     /ApEinfo_label="full attP "
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6113..6114
                     /locus_tag="recombination site"
                     /label="recombination site"
                     /ApEinfo_label="recombination site"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1090..1491
                     /locus_tag="myo-2 3' UTR"
                     /label="myo-2 3' UTR"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(3674..4361)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(36..69)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6225..6510
                     /locus_tag="rps-7 3' UTR"
                     /label="rps-7 3' UTR"
                     /ApEinfo_label="rps-7 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5968..5990
                     /locus_tag="synthetic sgRNA site A"
                     /label="synthetic sgRNA site A"
                     /ApEinfo_label="synthetic sgRNA site A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6..30
                     /locus_tag="synthetic sGRNA site B"
                     /label="synthetic sGRNA site B"
                     /ApEinfo_label="synthetic sGRNA site B"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           complement(1618..1969)
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     6092..6133
                     /locus_tag="attP phiC31"
                     /label="attP phiC31"
                     /ApEinfo_label="attP phiC31"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(3063..3425)
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note=""/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     127..159
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(1995..3020)
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1971..1991
                     /locus_tag="attB2"
                     /label="attB2"
                     /ApEinfo_label="attB2"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     complement(3026..3046)
                     /locus_tag="attB1"
                     /label="attB1"
                     /ApEinfo_label="attB1"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     transit_peptide 1027..1083
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1978..1984
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(3033..3039)
                     /locus_tag="Gateway recomb1"
                     /label="Gateway recomb1"
                     /ApEinfo_label="Gateway recomb1"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 GTACGGCGAC GTAACGTGAG ATAGGCGGaa cccggGAAGT TCCTATACta tttgaaGAAT
       61 AGGAACTTCt gcaggaattc gatatcGGTG AAGTTCCTAT TCtctagaaa GTATAGGAAC
      121 TTCAAAATGG GATCCCCAAA GAAGAAGCGT AAGGTGTCGA TGAGTAAAGG AGAAGAATTG
      181 TTCACTGGAG TTGTCCCAAT CCTCGTCGAG CTCGACGGAG ACGTCAACGG ACACAAGTTC
      241 TCCGTCTCCG GAGAGGGAGA GGGAGACGCC ACCTACGGAA AGCTCACCCT CAAGTTCATC
      301 TGCACCACCG GAAAGCTCCC AGTCCCATGG CCAACCCTCG TCACCACCTT CTGCTACGGA
      361 GTCCAATGCT TCTCCCGTTA CCCAGACCAC ATGAAGCGTC ACGACTTCTT CAAGTCCGCC
      421 ATGCCAGAGG GATACGTCCA AGAGCGTACC ATCTTCTTCA AGgtaagttt aaacatatat
      481 atactaacta ctgattattt aaattttcag GACGACGGAA ACTACAAGAC CCGTGCCGAG
      541 GTCAAGTTCG AGGGAGACAC CCTCGTCAAC CGTATCGAGC TCAAGgtaag tttaaacagt
      601 tcggtactaa ctaaccatac atatttaaat tttcagGGAA TCGACTTCAA GGAGGACGGA
      661 AACATCCTCG GACACAAGCT CGAGTACAAC TACAACTCCC ACAACGTCTA CATCATGGCC
      721 GACAAGCAAA AGAACGGAAT CAAGGTCAAC TTCAAGgtaa gtttaaacat gattttacta
      781 actaactaat ctgatttaaa ttttcagATC CGTCACAACA TCGAGGACGG ATCCGTCCAA
      841 CTCGCCGACC ACTACCAACA AAACACCCCA ATCGGAGACG GACCAGTCCT CCTCCCAGAC
      901 AACCACTACC TCTCCACCCA ATCCGCCCTC TCCAAGGACC CAAACGAGAA GCGTGACCAC
      961 ATGGTCCTCC TCGAGTTCGT CACCGCCGCC GGAATCACCC ACGGAATGGA CGAGCTCTAC
     1021 AAGGCCTCCC GTCGTCGTAA GGCCAACCCA ACCAAGCTCT CCGAGAACGC CAAGAAGCTC
     1081 GCCAAGtaga tcaactgctc taattccatc tcgtttctct cttttctttc aatctcttta
     1141 cccgtttctc ttcccctacg tcccccaaac ctaccactgc acagtaacac atacacattc
     1201 aacgtgtcac aataaaagcc catcaacacc ttctctgttg ttttttttct tcaaatgcat
     1261 ttttttcact ttgtttaata atttttacgc atcggatttg taggatagtg gaaatttgtc
     1321 cttcaatgct tcaattgatt cgacacttct caatattttt gatcaataaa ccattgattt
     1381 tctatttaga agaagatcac tgtaaataac accaaaaaca tcacagcaaa aaaactccca
     1441 ctcaattata ttactgtttt tattgaaact agaataaatt tgaaaatctt caaagcatac
     1501 acaattaaaa aaaacgaaaa aagaaaaagt cccgttgatt ctcaaactta aatttttaaa
     1561 cttgtctgaa ccatgttgta agtttgtaaa agtttcccag ttgtcctcga acaGGTCTAA
     1621 AAATTGCGGT CATAAACTGA AACGTAACAT ATGATAAGGT ATTTTGTGTG CGGTTTTTTC
     1681 TATGATGTTT CAAAAAAATT TATCAGAAGT AAAAAACATA AGAAGTGTAC ACAGAACATT
     1741 GTGTAGATTT TGGGATAGAA TTATTATTAA TGGGGGGAGG GAGCACAATT TTTTTCGACT
     1801 TTTTATTACG AATTAATTCT ATTTTTGAAT CTACACAATT TCATTGTTAG AGGTGACTTA
     1861 AAAGAAGCTA AAAAACAAAG AAATTAAGAG AAGTTTTTTG ATAATAACAA AAATAGGGGG
     1921 TGGGAGCACA GGGAGAAAGA GCATGTAGGG ATGTTGAAGA GTAATTGGAC CCACTTTGTA
     1981 CAAGAAAGCT GGGTCTATTC CTTTGCCCTC GGACGAGTGC TGGGGCGTCG GTTTCCACTA
     2041 TCGGCGAGTA CTTCTACACA GCCATCGGTC CAGACGGCCG CGCTTCTGCG GGCGATTTGT
     2101 GTACGCCCGA CAGTCCCGGC TCCGGATCGG ACGATTGCGT CGCATCGACC CTGCGCCCAA
     2161 GCTGCATCAT CGAAATTGCC GTCAACCAAG CTCTGATAGA GTTGGTCAAG ACCAATGCGG
     2221 AGCATATACG CCCGGAGCCG CGGCGATCCT GCAAGCTCCG GATGCCTCCG CTCGAAGTAG
     2281 CGCGTCTGCT GCTCCATACA AGCCAACCAC GGCCTCCAGA AGAAGATGTT GGCGACCTCG
     2341 TATTGGGAAT CCCCGAACAT CGCCTCGCTC CAGTCAATGA CCGCTGTTAT GCGGCCATTG
     2401 TCCGTCAGGA CATTGTTGGA GCCGAAATCC GCGTGCACGA GGTGCCGGAC TTCGGGGCAG
     2461 TCCTCGGCCC AAAGCATCAG CTCATCGAGA GCCTGCGCGA CGGACGCACT GACGGTGTCG
     2521 TCCATCACAG TTTGCCAGTG ATACACATGG GGATCAGCAA TCGCGCATAT GAAATCACGC
     2581 CATGTAGTGT ATTGACCGAT TCCTTGCGGT CCGAATGGGC CGAACCCGCT CGTCTGGCTA
     2641 AGATCGGCCG CAGCGATCGC ATCCATGGCC TCCGCGACCG GCTGCAGAAC AGCGGGCAGT
     2701 TCGGTTTCAG GCAGGTCTTG CAACGTGACA CCCTGTGCAC GGCGGGAGAT GCAATAGGTC
     2761 AGGCTCTCGC TGAATTCCCC AATGTCAAGC ACTTCCGGAA TCGGGAGCGC GGCCGATGCA
     2821 AAGTGCCGAT AAACATAACG ATCTTTGTAG AAACCATCGG CGCAGCTATT TACCCGCAGG
     2881 ACATATCCAC GCCCTCCTAC ATCGAAGCTG AAAGCACGAG ATTCTTCGCC CTCCGAGAGC
     2941 TGCATCAGGT CGGAGACGCT GTCGAACTTT TCGATCAGAA ACTTCTCGAC AGACGTCGCG
     3001 GTGAGTTCAG GCTTTTTCAT GGAGCCTGCT TTTTTGTACA AACTTGCGGC ACCGCCTGAC
     3061 ATATTACCTT AAAATTCAAA AATTAATTTC AGATCAGTCA TAACCAAGAA AACAAAATAA
     3121 ACGTTAGGAC AGAAAATTCA AATTAATTAA AAATTATGCT GAATAAGAAA ATTACATGCA
     3181 AAGCCTTATT TTCCTCATAG AAACAGTTGA ATAACATATT TTTTAATTGA AAACATAGCA
     3241 AAATTCGTGG TAAAATTTGT CAAAACCTTG GAAAAAATCG AAAAAAGAAC ATTTCCCGAT
     3301 GCATTTTCCT ATTTCTCGTT GCCGACAACG TATAAACGTT GAATACTCAG TCAACGAATT
     3361 TGGCTGCTGG CTTGGACTCT CGCACGGAAG AGACGCGTGT GTAGATTTAC GACGAAAGCA
     3421 AAAATgatcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3481 aacgcgcggg gagaggcggt ttgcgtattg ggcgaattcc gcttcctcgc tcactgactc
     3541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3961 ccccattcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4561 cttaccatct ggccccagtg ctgcaatgat accgcgagat ccacgctcac cggctccaga
     4621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     5461 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     5521 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     5581 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     5641 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     5701 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     5761 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     5821 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     5881 aatgcgccgc tacagggcgc gtcgtcggag ctcATAACTT CGTATAGCAT ACATTATACG
     5941 AAGTTATgcg gccgctctag aaCTAGTGGC TGACATCCAG TAGACGGAGG ctaCCGGTgt
     6001 actgacggac acaccgaagc cccggcggca accctcagcg gatgccccgg ggcttcacgt
     6061 tttcccaggt cagaagcggt tttcgggagt aGTGCCCCAA CTGGGGTAAC CTTTGAGTTC
     6121 TCTCAGTTGG GGGcgtaggg tcgccgacat gacacaaggg gttgtgaccg gggtggacac
     6181 gtacgcgggt gcttacgacc gtcagtcgcg cgagcgcgaC AATTGTCTAA ACATTTTTTG
     6241 TTGACTTACA ATAAATTGTT GATATAATTT ATTGTGAAAT GGGTATTCTT GAATGATTTG
     6301 ATTTTAACGA AGAGAACGCT GAAATCAACT GAACACCTCG AATTGAGATT TTGGGGATTT
     6361 TATACCAAGT TATCGACGAC GCAAATGGTG CAAACTTTAT ATTTTCAAAT ATTCTGGAAG
     6421 CATTCAATGG TCTTTTAAGA AACGGACAAC TCCATTCTTG ATATGCAATG TTTTTTAAAA
     6481 GAAATCTCTT TAATCGAGAC GGGCGCATAT agaaCCTAGG TGGCGAAGAG CCTGCAggta
     6541 accACGCGtt GCatGCTCTT CC   
//
