LOCUS       pHygRP12__4592_         6549 bp    DNA        circular     31-JUL-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Ligation reaction:
COMMENT     pHygR2 [4388] from 3105 (XhoI*) to 2535 (SpeI)
COMMENT     pHygRP3 [4483] from 4367 (SpeI) to 5516 (XhoI*)
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(1033..1692)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2153..2459)
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2488..2521
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    2573..2793
                     /locus_tag="full attP "
                     /label="full attP "
                     /ApEinfo_label="full attP "
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4213..4614
                     /locus_tag="myo-2 3' UTR"
                     /label="myo-2 3' UTR"
                     /ApEinfo_label="myo-2 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2542..2564
                     /locus_tag="synthetic sgRNA site A"
                     /label="synthetic sgRNA site A"
                     /ApEinfo_label="synthetic sgRNA site A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           complement(4741..5092)
                     /locus_tag="unc-54 3'UTR"
                     /label="unc-54 3'UTR"
                     /ApEinfo_label="unc-54 3'UTR"
                     /ApEinfo_fwdcolor="#990000"
                     /ApEinfo_revcolor="#cc0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(6149..6169)
                     /locus_tag="attB1"
                     /label="attB1"
                     /ApEinfo_label="attB1"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(248..935)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_recomb     5094..5114
                     /locus_tag="attB2"
                     /label="attB2"
                     /ApEinfo_label="attB2"
                     /ApEinfo_fwdcolor="#dda0dd"
                     /ApEinfo_revcolor="#dda0dd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2687..2688
                     /locus_tag="recombination site"
                     /label="recombination site"
                     /ApEinfo_label="recombination site"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(6186..6548)
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note=""/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;"
                     /locus_tag="Prps-0"
                     /label="Prps-0"
                     /ApEinfo_label="Prps-0"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3174..3207)
                     /locus_tag="FRT3"
                     /label="FRT3"
                     /ApEinfo_label="FRT3"
                     /ApEinfo_fwdcolor="#804000"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(5118..6143)
                     /note="hygromycinR"
                     /locus_tag="hygR"
                     /label="hygR"
                     /ApEinfo_label="hygR"
                     /ApEinfo_fwdcolor="#006666"
                     /ApEinfo_revcolor="#006666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3144..3168
                     /locus_tag="synthetic sGRNA site B"
                     /label="synthetic sGRNA site B"
                     /ApEinfo_label="synthetic sGRNA site B"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2799..3084
                     /locus_tag="rps-7 3' UTR"
                     /label="rps-7 3' UTR"
                     /ApEinfo_label="rps-7 3' UTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2666..2707
                     /locus_tag="attP phiC31"
                     /label="attP phiC31"
                     /ApEinfo_label="attP phiC31"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#800000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3228..3261
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="#800000"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide 4150..4206
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5101..5107
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     sig_peptide     3274..3296
                     /locus_tag="SV40 nls"
                     /label="SV40 nls"
                     /ApEinfo_label="SV40 nls"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    join(3298..3450,3502..3714,3766..3936,3988..4143)
                     /locus_tag="mScarlet-I-C1"
                     /label="mScarlet-I-C1"
                     /ApEinfo_label="mScarlet-I-C1"
                     /ApEinfo_fwdcolor="#ff6771"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(6156..6162)
                     /locus_tag="Gateway recomb1"
                     /label="Gateway recomb1"
                     /ApEinfo_label="Gateway recomb1"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 atcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
       61 cggggagagg cggtttgcgt attgggcgaa ttccgcttcc tcgctcactg actcgctgcg
      121 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      181 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      241 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      301 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      361 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      421 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      481 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccat
      541 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      601 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      661 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      721 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      781 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      841 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      901 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      961 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1021 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1081 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1141 atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc cagatttatc
     1201 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1261 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1321 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1381 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1441 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1501 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1561 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1621 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1681 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1741 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1801 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1861 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1921 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1981 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     2041 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2101 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2161 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2221 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2281 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     2341 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     2401 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     2461 ccgctacagg gcgcgtcgtc ggagctcATA ACTTCGTATA GCATACATTA TACGAAGTTA
     2521 Tgcggccgct ctagaaCTAG TGGCTGACAT CCAGTAGACG GAGGctaCCG GTgtactgac
     2581 ggacacaccg aagccccggc ggcaaccctc agcggatgcc ccggggcttc acgttttccc
     2641 aggtcagaag cggttttcgg gagtaGTGCC CCAACTGGGG TAACCTTTGA GTTCTCTCAG
     2701 TTGGGGGcgt agggtcgccg acatgacaca aggggttgtg accggggtgg acacgtacgc
     2761 gggtgcttac gaccgtcagt cgcgcgagcg cgaCAATTGT CTAAACATTT TTTGTTGACT
     2821 TACAATAAAT TGTTGATATA ATTTATTGTG AAATGGGTAT TCTTGAATGA TTTGATTTTA
     2881 ACGAAGAGAA CGCTGAAATC AACTGAACAC CTCGAATTGA GATTTTGGGG ATTTTATACC
     2941 AAGTTATCGA CGACGCAAAT GGTGCAAACT TTATATTTTC AAATATTCTG GAAGCATTCA
     3001 ATGGTCTTTT AAGAAACGGA CAACTCCATT CTTGATATGC AATGTTTTTT AAAAGAAATC
     3061 TCTTTAATCG AGACGGGCGC ATATagaaCC TAGTGGAACC GAAGAGCCTG CAggtaaccA
     3121 CGCGttGCat GCTCTTCCGT ACGGCGACGT AACGTGAGAT AGGCGGaacc cggGAAGTTC
     3181 CTATACtatt tgaaGAATAG GAACTTCtgc aggaattcga tatcGGTGAA GTTCCTATTC
     3241 tctagaaaGT ATAGGAACTT CAAAATGGGA TCCCCAAAGA AGAAGCGTAA GGTGTCgATG
     3301 GTTTCCAAGG GAGAGGCCGT CATCAAGGAG TTCATGCGTT TCAAGGTCCA CATGGAGGGA
     3361 TCCATGAACG GACACGAGTT CGAGATCGAG GGAGAGGGAG AGGGACGTCC ATACGAGGGA
     3421 ACCCAAACCG CCAAGCTCAA GGTCACCAAG gtaagtttaa acatatatat actaactaac
     3481 cctgattatt taaattttca gGGAGGACCA CTCCCATTCT CCTGGGACAT CCTCTCCCCA
     3541 CAATTCATGT ACGGATCCCG TGCCTTCACC AAGCACCCAG CCGACATCCC AGACTACTAC
     3601 AAGCAATCCT TCCCAGAGGG ATTCAAGTGG GAGCGTGTCA TGAACTTCGA GGACGGAGGA
     3661 GCCGTCACCG TCACCCAAGA CACCTCCCTC GAGGACGGAA CCCTCATCTA CAAGgtaagt
     3721 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcagGTCAA GCTCCGTGGA
     3781 ACCAACTTCC CACCAGACGG ACCAGTCATG CAAAAGAAGA CCATGGGATG GGAGGCCTCC
     3841 ACCGAGCGTC TCTACCCAGA GGACGGAGTC CTCAAGGGAG ACATCAAGAT GGCCCTCCGT
     3901 CTCAAGGACG GAGGACGTTA CCTCGCCGAC TTCAAGgtaa gtttaaacat gattttacta
     3961 actaactaat ctgatttaaa ttttcagACC ACCTACAAGG CCAAGAAGCC AGTCCAAATG
     4021 CCAGGAGCCT ACAACGTCGA CCGTAAGCTC GACATCACCT CCCACAACGA GGACTACACC
     4081 GTCGTCGAGC AATACGAGCG TTCCGAGGGA CGTCACTCCA CCGGAGGAAT GGACGAGCTC
     4141 TACAAGGCCT CCCGTCGTCG TAAGGCCAAC CCAACCAAGC TCTCCGAGAA CGCCAAGAAG
     4201 CTCGCCAAGt agatcaactg ctctaattcc atctcgtttc tctcttttct ttcaatctct
     4261 ttacccgttt ctcttcccct acgtccccca aacctaccac tgcacagtaa cacatacaca
     4321 ttcaacgtgt cacaataaaa gcccatcaac accttctctg ttgttttttt tcttcaaatg
     4381 catttttttc actttgttta ataattttta cgcatcggat ttgtaggata gtggaaattt
     4441 gtccttcaat gcttcaattg attcgacact tctcaatatt tttgatcaat aaaccattga
     4501 ttttctattt agaagaagat cactgtaaat aacaccaaaa acatcacagc aaaaaaactc
     4561 ccactcaatt atattactgt ttttattgaa actagaataa atttgaaaat cttcaaagca
     4621 tacacaatta aaaaaaacga aaaaagaaaa agtcccgttg attctcaaac ttaaattttt
     4681 aaacttgtct gaaccatgtt gtaagtttgt aaaagtttcc cagttgtcct cgaacaGGTC
     4741 TAAAAATTGC GGTCATAAAC TGAAACGTAA CATATGATAA GGTATTTTGT GTGCGGTTTT
     4801 TTCTATGATG TTTCAAAAAA ATTTATCAGA AGTAAAAAAC ATAAGAAGTG TACACAGAAC
     4861 ATTGTGTAGA TTTTGGGATA GAATTATTAT TAATGGGGGG AGGGAGCACA ATTTTTTTCG
     4921 ACTTTTTATT ACGAATTAAT TCTATTTTTG AATCTACACA ATTTCATTGT TAGAGGTGAC
     4981 TTAAAAGAAG CTAAAAAACA AAGAAATTAA GAGAAGTTTT TTGATAATAA CAAAAATAGG
     5041 GGGTGGGAGC ACAGGGAGAA AGAGCATGTA GGGATGTTGA AGAGTAATTG GACCCACTTT
     5101 GTACAAGAAA GCTGGGTCTA TTCCTTTGCC CTCGGACGAG TGCTGGGGCG TCGGTTTCCA
     5161 CTATCGGCGA GTACTTCTAC ACAGCCATCG GTCCAGACGG CCGCGCTTCT GCGGGCGATT
     5221 TGTGTACGCC CGACAGTCCC GGCTCCGGAT CGGACGATTG CGTCGCATCG ACCCTGCGCC
     5281 CAAGCTGCAT CATCGAAATT GCCGTCAACC AAGCTCTGAT AGAGTTGGTC AAGACCAATG
     5341 CGGAGCATAT ACGCCCGGAG CCGCGGCGAT CCTGCAAGCT CCGGATGCCT CCGCTCGAAG
     5401 TAGCGCGTCT GCTGCTCCAT ACAAGCCAAC CACGGCCTCC AGAAGAAGAT GTTGGCGACC
     5461 TCGTATTGGG AATCCCCGAA CATCGCCTCG CTCCAGTCAA TGACCGCTGT TATGCGGCCA
     5521 TTGTCCGTCA GGACATTGTT GGAGCCGAAA TCCGCGTGCA CGAGGTGCCG GACTTCGGGG
     5581 CAGTCCTCGG CCCAAAGCAT CAGCTCATCG AGAGCCTGCG CGACGGACGC ACTGACGGTG
     5641 TCGTCCATCA CAGTTTGCCA GTGATACACA TGGGGATCAG CAATCGCGCA TATGAAATCA
     5701 CGCCATGTAG TGTATTGACC GATTCCTTGC GGTCCGAATG GGCCGAACCC GCTCGTCTGG
     5761 CTAAGATCGG CCGCAGCGAT CGCATCCATG GCCTCCGCGA CCGGCTGCAG AACAGCGGGC
     5821 AGTTCGGTTT CAGGCAGGTC TTGCAACGTG ACACCCTGTG CACGGCGGGA GATGCAATAG
     5881 GTCAGGCTCT CGCTGAATTC CCCAATGTCA AGCACTTCCG GAATCGGGAG CGCGGCCGAT
     5941 GCAAAGTGCC GATAAACATA ACGATCTTTG TAGAAACCAT CGGCGCAGCT ATTTACCCGC
     6001 AGGACATATC CACGCCCTCC TACATCGAAG CTGAAAGCAC GAGATTCTTC GCCCTCCGAG
     6061 AGCTGCATCA GGTCGGAGAC GCTGTCGAAC TTTTCGATCA GAAACTTCTC GACAGACGTC
     6121 GCGGTGAGTT CAGGCTTTTT CATGGAGCCT GCTTTTTTGT ACAAACTTGC GGCACCGCCT
     6181 GACATATTAC CTTAAAATTC AAAAATTAAT TTCAGATCAG TCATAACCAA GAAAACAAAA
     6241 TAAACGTTAG GACAGAAAAT TCAAATTAAT TAAAAATTAT GCTGAATAAG AAAATTACAT
     6301 GCAAAGCCTT ATTTTCCTCA TAGAAACAGT TGAATAACAT ATTTTTTAAT TGAAAACATA
     6361 GCAAAATTCG TGGTAAAATT TGTCAAAACC TTGGAAAAAA TCGAAAAAAG AACATTTCCC
     6421 GATGCATTTT CCTATTTCTC GTTGCCGACA ACGTATAAAC GTTGAATACT CAGTCAACGA
     6481 ATTTGGCTGC TGGCTTGGAC TCTCGCACGG AAGAGACGCG TGTGTAGATT TACGACGAAA
     6541 GCAAAAATg     
//
