LOCUS       V100_Td_ITR_miR1        6686 bp    DNA     circular SYN 12-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 31, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
COMMENT     LOCUS       pAAV-Ef1a-DIO-EY        6323 bp    DNA     circular     
            29-MAR-2015
            SOURCE
              ORGANISM
            KEYWORDS
            COMMENT     This file is created by Vector NTI





                        http://www.invitrogen.com/





                        ORIGDB|GenBank





                        VNTDATE|484398094|





                        VNTDBDATE|508402734|





                        LSOWNER|





                        VNTNAME|pAAV-EF1a-double floxed eYFP-WPRE|





                        VNTAUTHORNAME|Demo User|





                        VNTOAUTHORNAME|Feng Zhang|





                        Vector_NTI_Display_Data_(Do_Not_Edit!)





                        (SXF 





                         (CGexDoc ""pAAV-EF1a-double floxed eYFP-WPRE"" 0 6323 
            





                          (CDBMol 0 0 1 1 1 0 0 0 0 """" """" 0 0 0 0 (CObList) 
            (CObList) (CObLi
                        

                        


                        



                        




                        st) 





                           (CObList) -1 """") 





                          (CDocSetData 1 1 0 0 0 1 ""MAIN"" 1 1 1 1 0 0 1 1 0 1 
            10 10 42949672
                        

                        


                        



                        




                        95 50 0 





                           1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
            





                           (CStringList ""AarI"" ""AatII"" ""Acc65I"" ""AccI"" 
            ""AceIII"" ""AciI"" ""AclI
                        

                        


                        



                        




                        "" 





                            ""AcuI"" ""AfeI"" ""AflII"" ""AflIII"" ""AgeI"" 
            ""AhdI"" ""AleI"" ""AloI"" ""AluI
                        

                        


                        



                        




                        "" 





                            ""AlwI"" ""AlwNI"" ""ApaBI"" ""ApaI"" ""ApaLI"" 
            ""ApoI"" ""AscI"" ""AseI"" ""AsiS
                        

                        


                        



                        




                        I"" 





                            ""AvaI"" ""AvaII"" ""AvrII"" ""BaeI"" ""BamHI"" 
            ""BanI"" ""BanII"" ""BbeI"" ""Bbr
                        

                        


                        



                        




                        7I"" 





                            ""BbsI"" ""BbvCI"" ""BbvI"" ""BccI"" ""BceAI"" 
            ""BcefI"" ""BcgI"" ""BciVI"" ""Bcl
                        

                        


                        



                        




                        I"" 





                            ""BfaI"" ""BfrBI"" ""BglI"" ""BglII"" ""BlpI"" 
            ""Bme1580I"" ""BmgBI"" ""BmrI"" ""
                        

                        


                        



                        




                        BmtI"" 





                            ""BplI"" ""BpmI"" ""Bpu10I"" ""BpuEI"" ""BsaAI"" 
            ""BsaBI"" ""BsaHI"" ""BsaI"" ""B
                        

                        


                        



                        




                        saJI"" 





                            ""BsaWI"" ""BsaXI"" ""BscAI"" ""BseMII"" ""BseRI"" 
            ""BseYI"" ""BsgI"" ""BsiEI"" 





                            ""BsiHKAI"" ""BsiWI"" ""BslI"" ""BsmAI"" ""BsmBI"" 
            ""BsmFI"" ""BsmI"" ""Bsp1286
                        

                        


                        



                        




                        I"" 





                            ""Bsp24I"" ""BspCNI"" ""BspEI"" ""BspHI"" ""BspMI"" 
            ""BsrBI"" ""BsrDI"" ""BsrFI
                        

                        


                        



                        




                        "" 





                            ""BsrGI"" ""BsrI"" ""BssHII"" ""BssSI"" ""BstAPI"" 
            ""BstBI"" ""BstEII"" ""BstF5
                        

                        


                        



                        




                        I"" 





                            ""BstKTI"" ""BstNI"" ""BstUI"" ""BstXI"" ""BstYI"" 
            ""BstZ17I"" ""Bsu36I"" ""Btg
                        

                        


                        



                        




                        I"" 





                            ""BthCI"" ""BtsI"" ""Cac8I"" ""CdiI"" ""ChaI"" 
            ""CjeI"" ""CjePI"" ""ClaI"" ""Csp6
                        

                        


                        



                        




                        I"" 





                            ""CviAII"" ""CviJI"" ""DdeI"" ""DpnI"" ""DraI"" 
            ""DraIII"" ""DrdI"" ""EaeI"" ""Ea
                        

                        


                        



                        




                        gI"" 





                            ""EarI"" ""EciI"" ""Eco57MI"" ""EcoHI"" ""EcoICRI"" 
            ""EcoNI"" ""EcoO109I"" ""Ec
                        

                        


                        



                        




                        oRI"" 





                            ""EcoRV"" ""EsaBC3I"" ""FalI"" ""FatI"" ""FauI"" 
            ""FmuI"" ""Fnu4HI"" ""FokI"" ""F
                        

                        


                        



                        




                        seI"" 





                            ""FspAI"" ""FspI"" ""GdiII"" ""HaeI"" ""HaeII"" 
            ""HaeIII"" ""HaeIV"" ""HgaI"" ""H
                        

                        


                        



                        




                        haI"" 





                            ""Hin4I"" ""HincII"" ""HindIII"" ""HinfI"" 
            ""HinP1I"" ""HpaI"" ""HpaII"" ""HphI
                        

                        


                        



                        




                        "" 





                            ""Hpy188I"" ""Hpy188III"" ""Hpy8I"" ""Hpy99I"" 
            ""HpyAV"" ""HpyCH4I"" ""HpyCH4
                        

                        


                        



                        




                        III"" 





                            ""HpyCH4IV"" ""HpyCH4V"" ""HpyF10VI"" ""KasI"" 
            ""KpnI"" ""LpnI"" ""MaeIII"" ""M
                        

                        


                        



                        




                        boI"" 





                            ""MboII"" ""MfeI"" ""MluI"" ""MlyI"" ""MmeI"" 
            ""MnlI"" ""MscI"" ""MseI"" ""MslI"" 





                            ""MspA1I"" ""MwoI"" ""NaeI"" ""NarI"" ""NciI"" 
            ""NcoI"" ""NdeI"" ""NgoMIV"" ""Nhe
                        

                        


                        



                        




                        I"" 





                            ""NlaIII"" ""NlaIV"" ""Nli3877I"" ""NotI"" ""NruI"" 
            ""NsiI"" ""NspI"" ""PacI"" ""
                        

                        


                        



                        




                        PciI"" 





                            ""PflMI"" ""PfoI"" ""PleI"" ""PmeI"" ""PmlI"" 
            ""PpiI"" ""Ppu10I"" ""PpuMI"" ""Psh
                        

                        


                        



                        




                        AI"" 





                            ""PsiI"" ""Psp03I"" ""PspGI"" ""PspOMI"" ""PsrI"" 
            ""PssI"" ""PstI"" ""PvuI"" ""Pv
                        

                        


                        



                        




                        uII"" 





                            ""RleAI"" ""RsaI"" ""RsrII"" ""SacI"" ""SacII"" 
            ""SalI"" ""SanDI"" ""SapI"" ""Sau
                        

                        


                        



                        




                        96I"" 





                            ""SbfI"" ""ScaI"" ""SciI"" ""ScrFI"" ""SelI"" 
            ""SexAI"" ""SfaNI"" ""SfcI"" ""SfiI
                        

                        


                        



                        




                        "" 





                            ""SfoI"" ""SgrAI"" ""SimI"" ""SmaI"" ""SmlI"" 
            ""SnaBI"" ""SpeI"" ""SphI"" ""SrfI""
                        

                        


                        



                        




                         





                            ""Sse232I"" ""Sse8647I"" ""SspD5I"" ""SspI"" 
            ""Sth132I"" ""StsI"" ""StuI"" ""St
                        

                        


                        



                        




                        yD4I"" 





                            ""StyI"" ""SwaI"" ""TaiI"" ""TaqI"" ""TaqII"" 
            ""TatI"" ""TauI"" ""TfiI"" ""TseI"" 





                            ""Tsp45I"" ""Tsp509I"" ""TspDTI"" ""TspGWI"" 
            ""TspRI"" ""Tth111I"" ""Tth111II
                        

                        


                        



                        




                        "" 





                            ""UnbI"" ""UthSI"" ""VpaK11AI"" ""XbaI"" ""XcmI"" 
            ""XhoI"" ""XmaI"" ""XmnI"" ""Zr
                        

                        


                        



                        




                        aI"") 





                           (CStringList ""atg"" ""gtg"") (CStringList ""taa"" 
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                        t) 1 





                           ""{(0,1),2}"" 0 0 """" 0 4294967295 0 0 0 0 0 0 0 
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                           (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 
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                          (CMolPar 0 0 0 0 0 1 6323 0 0 0 0 0 0 0 0) 
            (CStringList) (CStringL
                        

                        


                        



                        




                        ist) 





                          (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 
            50 0 6 4 3 7
                        

                        


                        



                        




                        ) 





                          (COAPar 25 250 50 0 6 4 3 7) 





                          (CObList #0=(CRSite (CStringList) ""AarI"" ""cacctgc""
            12 16 0 0 """") 





                           #1=(CRSite (CStringList) ""AatII"" ""gacgtc"" 6 0 2 
            2904 0 4512 0 0 ""
                        

                        


                        



                        




                        "") 





                           #2=(CRSite (CStringList) ""Acc65I"" ""ggtacc"" 2 0 1 
            1421 0 0 """") 





                           #3=(CRSite (CStringList) ""AccI"" ""gtmkac"" 3 0 1 
            1440 0 0 """") 





                           #4=(CRSite (CStringList) ""AceIII"" ""cagctc"" 14 18 
            9 1598 0 1748 1 




                        1931 0 





                               2252 0 2285 0 2727 0 4188 1 4385 1 5127 1 0 """")
            





                           #5=(CRSite (CStringList) ""AciI"" ""ccgc"" 2 4 79 38 
            0 143 1 147 0 34
                        

                        


                        



                        




                        3 1 380 





                               0 460 0 494 0 496 1 676 0 679 0 800 1 840 0 842 1
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                        1 5871 





                               0 5981 1 6102 1 6121 1 6248 1 6276 1 0 """") 
            





                           #6=(CRSite (CStringList) ""AclI"" ""aacgtt"" 3 0 4 
            436 0 4245 0 4829 




                        0 5202 





                               0 0 """") 





                           #7=(CRSite (CStringList) ""AcuI"" ""ctgaag"" 23 21 5 
            1311 0 1901 1 21
                        

                        


                        



                        




                        44 1 





                               4765 0 5777 1 0 """") 





                           #8=(CRSite (CStringList) ""AfeI"" ""agcgct"" 4 0 1 
            3052 0 0 """") 





                           #9=(CRSite (CStringList) ""AflII"" ""cttaag"" 2 0 1 
            621 0 0 """") 





                           #10=(CRSite (CStringList) ""AflIII"" ""acrygt"" 2 0 3
            151 0 875 0 631
                        

                        


                        



                        




                        9 0 0 





                                """") #11=(CRSite (CStringList) ""AgeI"" 
            ""accggt"" 2 0 1 321 0 0 




                        """") 





                           #12=(CRSite (CStringList) ""AhdI"" ""gacnnnnngtc"" 7 
            0 4 71 0 3184 0 




                        3658 0 





                                5431 0 0 """") 





                           #13=(CRSite (CStringList) ""AleI"" ""cacnnnngtg"" 6 0
            5 1938 0 2115 0
                        

                        


                        



                        




                         2265 0 





                                2295 0 3128 0 0 """") 





                           #14=(CRSite (CStringList) ""AloI"" ""gaacnnnnnntcc"" 
            26 21 2 3546 0 4
                        

                        


                        



                        




                        071 1 0 





                                """") 





                           #15=(CRSite (CStringList) ""AluI"" ""agct"" 3 0 35 12
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                        80 0 





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                         0 6019 





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                           #16=(CRSite (CStringList) ""AlwI"" ""ggatc"" 10 11 15
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                        22 1 





                                1435 0 1601 1 1800 0 3021 0 4792 0 4796 1 5113 0
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                         0 5673 





                                1 5675 0 5761 0 0 """") 





                           #17=(CRSite (CStringList) ""AlwNI"" ""cagnnnctg"" 7 0
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                           #18=(CRSite (CStringList) ""ApaBI"" ""gcannnnntgc"" 9
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                        ) 





                           #19=(CRSite (CStringList) ""ApaI"" ""gggccc"" 6 0 1 
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                           #20=(CRSite (CStringList) ""ApaLI"" ""gtgcac"" 2 0 4 
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                        59 0 





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                           #21=(CRSite (CStringList) ""ApoI"" ""raatty"" 2 0 6 
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                         0 2470 





                                0 4215 0 4226 0 0 """") 





                           #22=(CRSite (CStringList) ""AscI"" ""ggcgcgcc"" 3 0 1
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                           #23=(CRSite (CStringList) ""AseI"" ""attaat"" 3 0 1 
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                           #24=(CRSite (CStringList) ""AsiSI"" ""gcgatcgc"" 6 0 
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                           #25=(CRSite (CStringList) ""AvaI"" ""cycgrg"" 2 0 12 
            41 0 52 0 288 0 




                        390 0 





                                575 0 1090 0 1207 0 3057 0 3362 0 3672 0 3683 0 
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                           #26=(CRSite (CStringList) ""AvaII"" ""ggwcc"" 2 0 6 
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                         0 3572 





                                0 5067 0 5289 0 0 """") 





                           #27=(CRSite (CStringList) ""AvrII"" ""cctagg"" 2 0 0 
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                           #28=(CRSite (CStringList) ""BaeI"" ""acnnnngtayc"" 24
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                           #29=(CRSite (CStringList) ""BamHI"" ""ggatcc"" 2 0 1 
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                           #30=(CRSite (CStringList) ""BanI"" ""ggyrcc"" 2 0 12 
            249 0 324 0 696 




                        0 955 0 





                                1015 0 1180 0 1191 0 1421 0 2256 0 3728 0 3991 0
            5478 0 0 """"
                        

                        


                        



                        




                        ) 





                           #31=(CRSite (CStringList) ""BanII"" ""grgcyc"" 6 0 5 
            631 0 859 0 1070
                        

                        


                        



                        




                         0 2804 





                                0 3961 0 0 """") 





                           #32=(CRSite (CStringList) ""BbeI"" ""ggcgcc"" 6 0 2 
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                        "") 





                           #33=(CRSite (CStringList) ""Bbr7I"" ""gaagac"" 14 18 
            2 2974 1 3256 0 




                        0 """") 





                           #34=(CRSite (CStringList) ""BbsI"" ""gaagac"" 9 13 2 
            2979 1 3251 0 0 




                        """") 





                           #35=(CRSite (CStringList) ""BbvCI"" ""cctcagc"" 3 6 2
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                         """") 





                           #36=(CRSite (CStringList) ""BbvI"" ""gcagc"" 14 18 29
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                        1 703 1 





                                748 1 1045 1 1494 1 1720 0 1723 0 1749 1 1756 1 
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                        0 2519 





                                1 2775 1 2851 1 2947 1 3040 1 3703 1 3725 0 3862
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                        97 1 





                                5388 0 5691 1 5897 1 5900 1 5990 0 6322 1 0 
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                           #37=(CRSite (CStringList) ""BccI"" ""ccatc"" 10 11 13
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                        2 1 





                                1252 1 1724 1 1994 1 2246 1 3594 1 4017 1 4049 0
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                         1 5385 





                                1 0 """") 





                           #38=(CRSite (CStringList) ""BceAI"" ""acggc"" 18 20 
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                        8 1 





                                1133 1 1168 1 1706 1 1757 1 1799 1 1877 1 1967 1
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                         1 2207 





                                1 2219 1 2773 0 4005 0 5819 1 0 """") 





                           #39=(CRSite (CStringList) ""BcefI"" ""acggc"" 18 19 
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                        9 1 





                                1134 1 1169 1 1707 1 1758 1 1800 1 1878 1 1968 1
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                         1 2208 





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                           #40=(CRSite (CStringList) ""BcgI"" ""cgannnnnntgc"" 
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                        65 0 





                                4893 1 0 """") 





                           #41=(CRSite (CStringList) ""BciVI"" ""gtatcc"" 13 12 
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                        2520 1 





                                4594 0 6121 0 0 """") 





                           #42=(CRSite (CStringList) ""BclI"" ""tgatca"" 2 0 0 0
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                           #43=(CRSite (CStringList) ""BfaI"" ""ctag"" 2 0 12 
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                        734 0 





                                1434 0 2304 0 3181 0 3595 0 3879 0 5238 0 5573 0
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                        ) 





                           #44=(CRSite (CStringList) ""BfrBI"" ""atgcat"" 4 0 1 
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                           #45=(CRSite (CStringList) ""BglI"" ""gccnnnnnggc"" 8 
            0 6 43 0 1013 0 




                        1189 0 





                                1306 0 3688 0 5313 0 0 """") 





                           #46=(CRSite (CStringList) ""BglII"" ""agatct"" 2 0 2 
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                        "") 





                           #47=(CRSite (CStringList) ""BlpI"" ""gctnagc"" 3 0 1 
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                           #48=(CRSite (CStringList) ""Bme1580I"" ""gkgcmc"" 6 0
            11 254 0 859 0 




                        1881 0 





                                2130 0 2259 0 2664 0 2817 0 3135 0 4266 0 4763 0
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                        ) 





                           #49=(CRSite (CStringList) ""BmgBI"" ""cacgtc"" 4 4 0 
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                           #50=(CRSite (CStringList) ""BmrI"" ""actggg"" 12 11 3
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                        91 0 0 





                                """") #51=(CRSite (CStringList) ""BmtI"" 
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                         """") 





                           #52=(CRSite (CStringList) ""BplI"" ""gagnnnnnctc"" 25
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                        ) 





                           #53=(CRSite (CStringList) ""BpmI"" ""ctggag"" 23 21 5
            1613 1 1853 1 3
                        

                        


                        



                        




                        111 1 





                                3307 0 5362 0 0 """") 





                           #54=(CRSite (CStringList) ""Bpu10I"" ""cctnagc"" 3 6 
            4 1144 0 1671 1 




                        1689 1 





                                3354 0 0 """") 





                           #55=(CRSite (CStringList) ""BpuEI"" ""cttgag"" 23 21 
            7 666 0 918 1 13
                        

                        


                        



                        




                        52 1 





                                4827 0 5695 0 5936 1 6234 0 0 """") 





                           #56=(CRSite (CStringList) ""BsaAI"" ""yacgtr"" 4 0 3 
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                        2 0 0 





                                """") #57=(CRSite (CStringList) ""BsaBI"" 
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                        "") 





                           #58=(CRSite (CStringList) ""BsaHI"" ""grcgyc"" 3 0 7 
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                         0 3669 





                                0 3729 0 4509 0 4891 0 0 """") 





                           #59=(CRSite (CStringList) ""BsaI"" ""ggtctc"" 8 12 2 
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                        """") 





                           #60=(CRSite (CStringList) ""BsaJI"" ""ccnngg"" 2 0 26
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                         494 0 





                                662 0 840 0 989 0 990 0 1167 0 1326 0 1881 0 
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                         0 3672 





                                0 3683 0 6159 0 0 """") 





                           #61=(CRSite (CStringList) ""BsaWI"" ""wccggw"" 2 0 7 
            246 0 321 0 1424
                        

                        


                        



                        




                         0 1445 





                                0 5135 0 5966 0 6113 0 0 """") 





                           #62=(CRSite (CStringList) ""BsaXI"" ""acnnnnnctcc"" 
            22 19 2 3544 0 40
                        

                        


                        



                        




                        71 1 0 





                                """") 





                           #63=(CRSite (CStringList) ""BscAI"" ""gcatc"" 10 12 
            15 1804 1 1903 1 




                        1918 1 





                                2192 0 3046 0 3084 0 3173 0 3728 1 3764 0 4280 1
            4370 0 4733
                        

                        


                        



                        




                         1 4978 





                                0 5173 1 6225 1 0 """") 





                           #64=(CRSite (CStringList) ""BseMII"" ""ctcag"" 16 14 
            18 23 1 98 0 115
                        

                        


                        



                        




                        8 0 





                                1266 1 1388 0 1685 0 1703 0 3015 0 3368 0 3412 0
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                         1 3707 





                                0 4283 0 4944 0 5461 1 5627 1 6036 1 0 """") 
            





                           #65=(CRSite (CStringList) ""BseRI"" ""gaggag"" 17 15 
            3 2267 1 2576 1 




                        2637 0 





                                0 """") 





                           #66=(CRSite (CStringList) ""BseYI"" ""cccagc"" 2 6 4 
            669 1 867 0 3505
                        

                        


                        



                        




                         1 6015 





                                0 0 """") 





                           #67=(CRSite (CStringList) ""BsgI"" ""gtgcag"" 23 21 5
            439 0 797 1 174
                        

                        


                        



                        




                        8 1 





                                2169 0 3312 0 0 """") 





                           #68=(CRSite (CStringList) ""BsiEI"" ""cgrycg"" 5 0 6 
            147 0 1013 0 358
                        

                        


                        



                        




                        3 0 





                                4913 0 5062 0 5985 0 0 """") 





                           #69=(CRSite (CStringList) ""BsiHKAI"" ""gwgcwc"" 6 0 
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                        4266 0 





                                4763 0 4848 0 6009 0 0 """") 





                           #70=(CRSite (CStringList) ""BsiWI"" ""cgtacg"" 2 0 0 
            0 """") 





                           #71=(CRSite (CStringList) ""BslI"" ""ccnnnnnnngg"" 8 
            0 37 59 0 131 0 




                        259 0 





                                260 0 334 0 396 0 397 0 512 0 582 0 663 0 917 0 
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                         0 2952 





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                        93 0 





                                5841 0 6120 0 6286 0 6304 0 0 """") 





                           #72=(CRSite (CStringList) ""BsmAI"" ""gtctc"" 7 11 11
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                         0 1280 





                                1 2617 0 3418 1 3460 0 4394 0 4436 1 4589 1 5365
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                           #73=(CRSite (CStringList) ""BsmBI"" ""cgtctc"" 8 12 3
            3418 1 4394 0 4
                        

                        


                        



                        




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FEATURES             Location/Qualifiers
     source          1..6686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=L-ITR
     misc_feature    140..1266
                     /label=KC103F
                     /note="KC103F"
     promoter        153..1252
                     /label=CAG
                     /note="CAG"
     primer_bind     153..188
                     /label=Primer 2
     primer_bind     723..745
                     /label=CCGAAAGTTTCCTTTTATGGCGA
     primer_bind     869..889
                     /label=CCGCGTTACTCCCACAGGTGA
     primer_bind     953..975
                     /label=Primer 5
     primer_bind     1262..1281
                     /label=YP-primer1
     misc_feature    1274..2704
                     /label=Tdtomato
     primer_bind     complement(1407..1422)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(2133..2151)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    2715..2736
                     /label=Mir122 TS
     misc_feature    2743..2764
                     /label=Mir122 TS
     misc_feature    2771..2792
                     /label=Mir122 TS
     misc_feature    2803..3411
                     /label=WPRE
     misc_feature    complement(2840..2867)
                     /label=KC17R
                     /note="KC17R"
     misc_feature    3168..3187
                     /label=WPRE forward
                     /note="WPRE forward"
     misc_feature    complement(3268..3287)
                     /label=WPRE reverse
                     /note="WPRE reverse "
     polyA_signal    3431..3909
                     /label=hGH polyA
     misc_feature    3949..4089
                     /label=R-ITR
     rep_origin      4181..4487
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    5006..5863
                     /label=AmpR
     rep_origin      6014..6681
                     /direction=RIGHT
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      541 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      601 ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      661 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      721 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      781 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      841 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      901 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      961 tggctgcgtg aaagccttga ggggctccgg gagcgccggc aggaaggaaa tgggcgggga
     1021 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1081 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1141 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1201 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgcggcccaa
     1261 cggtaccgcc accatggtga gtaagggcga ggaagtgatc aaagagttca tgcggtttaa
     1321 ggtgagaatg gaaggaagca tgaacggcca cgagttcgaa attgagggag aaggagaggg
     1381 acggccctac gagggcaccc agacagccaa gctgaaagtg acaaagggcg ggcctctgcc
     1441 attcgcttgg gacatcctga gcccacagtt tatgtacggc tccaaggcct atgtgaaaca
     1501 tccagctgac attcccgatt ataagaaact gagcttcccc gaggggttta agtgggaaag
     1561 agtgatgaac ttcgaggacg gaggcctggt gactgtgacc caggacagct ccctgcagga
     1621 tgggaccctg atctacaagg tgaaaatgag agggacaaat tttccccctg atggacctgt
     1681 gatgcagaag aaaactatgg gatgggaggc ctccaccgaa aggctgtatc cacgcgacgg
     1741 ggtgctgaaa ggagaaatcc accaggctct gaagctgaaa gatgggggac attacctggt
     1801 ggagttcaag acaatctaca tggccaagaa acctgtgcag ctgccaggct actattacgt
     1861 ggacacaaaa ctggatatca cttcacacaa cgaggactac actattgtgg agcagtatga
     1921 acggagcgag gggagacacc atctgttcct gggccatggg actggaagta ccggctcagg
     1981 gtctagtgga accgcctcaa gcgaggataa caatatggct gtgatcaaag agttcatgag
     2041 gtttaaggtg cgcatggagg gcagcatgaa tgggcacgaa tttgagattg aaggagaggg
     2101 cgaagggagg ccttacgagg gcacacagac tgccaagctg aaagtgacca agggaggacc
     2161 actgcctttc gcttgggata tcctgtctcc tcagtttatg tacggaagta aggcctatgt
     2221 caagcatccc gctgacattc ctgattacaa gaaactgtct ttcccagagg gctttaagtg
     2281 ggagagagtg atgaattttg aagatggagg cctggtgacc gtgacacagg actcctctct
     2341 gcaggatggc actctgatct acaaagtcaa aatgcgcggc accaattttc cacccgatgg
     2401 gcccgtgatg cagaagaaaa caatggggtg ggaggccagc actgaacggc tgtatcctag
     2461 agacggagtg ctgaagggcg aaatccacca ggccctgaag ctgaaagacg gcggccacta
     2521 cctggtggag ttcaaaacca tctacatggc caagaaacca gtgcagctgc ccggctatta
     2581 ctatgtggac accaagctgg atatcacatc ccacaatgaa gactacacca ttgtggaaca
     2641 gtatgagagg tctgaaggac gccaccatct gtttctgtac ggcatggatg agctgtataa
     2701 gtgaattcac cggtcaaaca ccattgtcac actccatact atcaaacacc attgtcacac
     2761 tccagatcaa caaacaccat tgtcacactc cacgccggcg aagcttatcg ataatcaacc
     2821 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2881 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2941 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3001 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3061 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3121 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3181 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctatgt
     3241 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3301 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3361 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gagcgctgct
     3421 cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     3481 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     3541 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     3601 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     3661 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     3721 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     3781 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     3841 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     3901 cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag gaacccctag
     3961 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     4021 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     4081 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     4141 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4201 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     4261 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4321 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     4381 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4441 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
     4501 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
     4561 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     4621 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     4681 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4741 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4801 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4861 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4921 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4981 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5041 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5101 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5161 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5221 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5281 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5341 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5401 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5461 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5521 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5581 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5641 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5701 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5761 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5821 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6661 cttttgctgg ccttttgctc acatgt
//
