LOCUS       pEKH035_pPF057_p        6391 bp DNA     circular SYN 01-OCT-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Princeton Network
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6391
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    24..827
                     /product="high_copy_origin"
                     /label=high_copy_origin
                     /note="color: #cccccc"
     misc_feature    1334..1359
                     /label=856
                     /note="color: #00ffff"
     primer_bind     1443..1492
                     /label=PEF179
                     /note="color: black; sequence: 
                     CATCGGTGATGTCGTATAAGAGACGTATAGGAGACCTATAGTGTCTTCGG; added: 
                     2022-09-06"
     primer_bind     1484..1530
                     /label=PEF173
                     /note="color: black; sequence: 
                     TGTCTTCGGGGCCGAGGCTCtGGtTCTCCACCTCCCGTCCCTAATCC; added: 
                     2022-08-19"
     CDS             1499..1807
                     /codon_start=1
                     /label=3xCD3epsilon_gblock
                     /note="color: #993366"
                     /translation="GSGSPPPVPNPDYEPIRKGQRDLYSGLNQRRIGSGSGPPPVPNPD
                     YEPIRKGQRDLYSGLNQRRIGSGSGPPPVPNPDYEPIRKGQRDLYSGLNQRRIGSGSG"
     CDS             1511..1594
                     /codon_start=1
                     /label=CD3-epsilon
                     /note="color: #ffcc00"
                     /translation="PPPVPNPDYEPIRKGQRDLYSGLNQRRI"
     CDS             1610..1693
                     /codon_start=1
                     /label=CD3-epsilon
                     /note="color: #ffcc00"
                     /translation="PPPVPNPDYEPIRKGQRDLYSGLNQRRI"
     CDS             1709..1792
                     /codon_start=1
                     /label=CD3-epsilon
                     /note="color: #ffcc00"
                     /translation="PPPVPNPDYEPIRKGQRDLYSGLNQRRI"
     primer_bind     complement(1746..1822)
                     /label=PEF071
                     /note="color: black; sequence: 
                     agtagctccgcttccGCCAGAACCagatCCTATTCGTCGTTGGTTCAATCCGCTGTAAA
                     GATCGCGTTGTCCCTTGC; added: 2021-12-06"
     primer_bind     complement(1775..1822)
                     /label=EKH_14
                     /note="color: black; sequence: 
                     agtagctccgcttccGCCAGAACCagatCCTATTCGTCGTTGGTTCAA; added: 
                     2022-01-13"
     misc_feature    1808..1873
                     /label=2A
                     /note="color: #00ffff"
     primer_bind     1808..1830
                     /label=EKH_15
                     /note="color: black; sequence: ggaagcggagctactaacttcag; 
                     added: 2022-01-13"
     CDS             1874..2557
                     /codon_start=1
                     /gene="Clover"
                     /label=Clover
                     /note="color: #ccffcc"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAA"
     CDS             2570..3343
                     /codon_start=1
                     /product="TANDEM SH2 DOMAIN FROM ELLIOT DINE'S ZAP70"
                     /label=tandem SH2
                     /note="color: #99cc00"
                     /translation="PDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGY
                     VLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRP
                     SGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWY
                     HSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCI
                     PEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSA"
     CDS             2594..2872
                     /codon_start=1
                     /label=SH2 1
                     /note="color: #993366"
                     /translation="FFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDV
                     RFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPC"
     CDS             3053..3328
                     /codon_start=1
                     /label=SH2 2
                     /note="color: #993366"
                     /translation="WYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKT
                     VYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEAC"
     primer_bind     complement(3323..3361)
                     /label=PEF174
                     /note="color: black; sequence: 
                     TCTTTCTAGGGTTAAtcaGGCACTGCTGTTGGGGCAGGC; added: 2022-08-19"
     misc_feature    3347..3350
                     /label=TTAA
                     /note="color: #ff6600"
     misc_feature    complement(3351..3588)
                     /label=3' Transposon End
                     /note="color: #93949d; direction: LEFT"
     misc_feature    3589..3794
                     /label=3xP3-hsp70pro
                     /note="color: #0c71ff"
     primer_bind     complement(3636..3677)
                     /label=PEF180
                     /note="color: black; sequence: 
                     TACTCCGGCGGTCGAGGGTTCGAAATCGATAAGCTTGGATCC; added: 
                     2022-09-06"
     misc_feature    3814..4491
                     /label=dsRed2
                     /note="color: #ff0d1b"
     misc_feature    4492..4733
                     /label=SV40polyA
                     /note="color: #59d400"
     misc_feature    4734..5042
                     /label=5' Transposon End
                     /note="color: #93949d"
     primer_bind     complement(5007..5047)
                     /label=Primer 1
                     /note="color: black; sequence: 
                     CTACTCGACTGGAGCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGC; 
                     added: 2022-08-19"
     primer_bind     5032..5046
                     /label=PEF174
                     /note="color: black; sequence: 
                     TCTTTCTAGGGTTAAtcaGGCACTGCTGTTGGGGCAGGC; added: 2022-08-19"
     misc_feature    5043..5046
                     /label=TTAA
                     /note="color: #ff6600"
     misc_feature    complement(5048..5054)
                     /label=SapI site
                     /label=SapI site(1)
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    complement(5059..5065)
                     /label=SapI site
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     complement(5118..5135)
                     /label=PEF179
                     /note="color: black; sequence: 
                     CATCGGTGATGTCGTATAAGAGACGTATAGGAGACCTATAGTGTCTTCGG; added: 
                     2022-09-06"
     misc_feature    complement(5188..5207)
                     /label=855
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    5519..6367
                     /product="AmpR"
                     /label=AmpR
                     /note="color: #cccccc"
ORIGIN
        1 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgcgc
       61 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      121 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      181 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      241 gataccaaat actgttcttc tagtgtagcc gtagttagcc caccacttca agaactctgt
      301 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      361 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      421 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      481 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      541 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      601 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      661 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      721 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      781 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      841 gaccgagcgc agcgagtcag tgagcgagga agcggaaggc gagagtaggg aactgccagg
      901 catcaaacta agcagaaggc ccctgacgga tggccttttt gcgtttctac aaactctttc
      961 tgtgttgtaa aacgacggcc agtcttaagc tcgggccccc tgggcggttc tgataacgag
     1021 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag
     1081 tttagggaaa gagcatttgt cagaatattt aagggcgcct gtcactttgc ttgatatatg
     1141 agaattattt aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct
     1201 ttttcgattg atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat
     1261 atacaatatt tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg
     1321 gaaaatcagt ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa
     1381 actataagat gttatcagta tttattatgc atttagaata cgtactcagc gtctgggttc
     1441 cccatcggtg atgtcgtata agagacgtat aggagaccta tagtgtcttc ggggccgagg
     1501 ctctggttct ccacctcccg tccctaatcc cgattatgag cccattcgga aaggtcaaag
     1561 agatttgtat agtggcctca atcaaagaag aatcgggtca ggttccggcc cgccgccagt
     1621 cccaaacccc gactatgaac caatcaggaa aggccaacgg gatttgtact caggattgaa
     1681 tcagcgcagg ataggttccg gctccgggcc accacccgtc cctaacccag attacgaacc
     1741 catacgcaag ggacaacgcg atctttacag cggattgaac caacgacgaa taggatctgg
     1801 ttctggcgga agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga
     1861 gaaccctgga cctatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1921 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
     1981 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2041 gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc
     2101 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2161 gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga
     2221 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2281 catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata tcacggccga
     2341 caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg aggacggcag
     2401 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2461 gcccgacaac cactacctga gccatcagtc cgccctgagc aaagacccca acgagaagcg
     2521 cgatcacatg gtcctgctgg agttcgtgac cgccgccggc tccggctctc cagaccccgc
     2581 ggcgcacctg cccttcttct acggcagcat ctcgcgtgcc gaggccgagg agcacctgaa
     2641 gctggcgggc atggcggacg ggctcttcct gctgcgccag tgcctgcgct cgctgggcgg
     2701 ctatgtgctg tcgctcgtgc acgatgtgcg cttccaccac tttcccatcg agcgccagct
     2761 caacggcacc tacgccattg ccggcggcaa agcgcactgt ggaccggcag agctctgcga
     2821 gttctactcg cgcgaccccg acgggctgcc ctgcaacctg cgcaagccgt gcaaccggcc
     2881 gtcgggcctc gagccgcagc cgggggtctt cgactgcctg cgagacgcca tggtgcgtga
     2941 ctacgtgcgc cagacgtgga agctggaggg cgaggccctg gagcaggcca tcatcagcca
     3001 ggccccgcag gtggagaagc tcattgctac gacggcccac gagcggatgc cctggtacca
     3061 cagcagcctg acgcgtgagg aggccgagcg caaactttac tctggggcgc agaccgacgg
     3121 caagttcctg ctgaggccgc ggaaggagca gggcacatac gccctgtccc tcatctatgg
     3181 gaagacggtg taccactacc tcatcagcca agacaaggcg ggcaagtact gcattcccga
     3241 gggcaccaag tttgacacgc tctggcagct ggtggagtat ctgaagctga aggcggacgg
     3301 gctcatctac tgcctgaagg aggcctgccc caacagcagt gcctgattaa ccctagaaag
     3361 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
     3421 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
     3481 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
     3541 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttagg atctaattca
     3601 attagagact aattcaatta gagctaattc aattaggatc caagcttatc gatttcgaac
     3661 cctcgaccgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa
     3721 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
     3781 agctaaacaa tcggctcgaa gccggtcgcc accatggcct cctccgagga cgtcatcaag
     3841 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
     3901 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     3961 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
     4021 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
     4081 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     4141 gactcctccc tccaggacgg ctccttcatc tacaaggtga agttcatcgg cgtgaacttc
     4201 ccctccgacg gccccgtaat gcagaagaag actatgggct gggaggcgtc caccgagcgc
     4261 ctgtaccccc gcgacggcgt gctgaagggc gagatccaca aggccctgaa gctgaaggac
     4321 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg
     4381 cccggctact actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc
     4441 atcgtggagc agtacgagcg cgccgagggc cgccaccacc tgttcctgta gcggccgcga
     4501 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     4561 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     4621 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4681 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttagatatct
     4741 ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat
     4801 aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac
     4861 tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac
     4921 gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta
     4981 gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag
     5041 ggttaaggaa gagccgtcgc tcttccccgg gaaaagaaga ccatatacgt ctcctatagg
     5101 tctcgtatac tgcttcctat acgacatcac cgatggggaa cacgaagcga tccagccccc
     5161 ctaattagcc cgggcgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
     5221 tggcagttcc ctactctcgc atggggagtc cccacactac catcggcgct acggcgtttc
     5281 acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaacaa
     5341 ggggtgttat gagccatatt caggtataaa tgggctcgcg ataatgttca gaattggtta
     5401 attggttgta acactgaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5461 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agaatatgag
     5521 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5581 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5641 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5701 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5761 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5821 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5881 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5941 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6001 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6061 agcgatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6121 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6181 ccttccggct ggctggttta ttgctgataa atccggagcc ggtgagcgtg gttctcgcgg
     6241 tatcatcgca gcgctggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6301 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6361 gattaagcat tggtaaaagc agagcattac g
//
