LOCUS       pEKH005_pHD-pYta        8403 bp DNA     circular SYN 20-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Princeton Network
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1499..2498
                     /chromosome="3L"
                     /mol_type="genomic DNA"
                     /note="/genotype=y[1]; Gr22b[1] Gr22d[1] cn[1] 
                     CG33964[R4.2]
                     bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]"
                     /note="color: #ffffff"
                     /db_xref="taxon:7227"
                     /organism="Drosophila melanogaster"
     source          6059..7058
                     /chromosome="3L"
                     /mol_type="genomic DNA"
                     /note="/genotype=y[1]; Gr22b[1] Gr22d[1] cn[1] 
                     CG33964[R4.2]
                     bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]"
                     /note="color: #ffffff"
                     /db_xref="taxon:7227"
                     /organism="Drosophila melanogaster"
     misc_feature    24..827
                     /product="high_copy_origin"
                     /label=high_copy_origin
                     /note="color: #cccccc"
     misc_feature    1334..1359
                     /label=856
                     /note="color: #00ffff"
     primer_bind     1443..1492
                     /label=PEF179
                     /note="color: black; sequence: 
                     CATCGGTGATGTCGTATAAGAGACGTATAGGAGACCTATAGTGTCTTCGG; added: 
                     2022-09-06"
     primer_bind     complement(1455..1513)
                     /label=EKH44
                     /note="color: black; sequence: 
                     ctccttgaccatcttTCGGCCCCGAAGACACTATAGGTCTCCTATACGTCTCTTATACG
                     ; added: 2023-09-20"
     misc_feature    1499..2498
                     /label=5' homology
                     /note="color: #ff6600"
     CDS             join(1499..1834,1920..2498)
                     /codon_start=1
                     /gene="btl"
                     /gene_synonym="0844/01; Btl; BTL; BTL/FGFR2; CG32134;
                     CG6714; CT20816; D-FGFR; dev; DFGF-R1; dFGFR; Dfr-2; DFR2; 
                     Dmel\CG32134; DmHD-311; dtk2; Dtk2; fgf-r; Fgf-r; Fgfr; 
                     FGFR; FGFR1; HD-311; l(3)00208; l(3)j2E11; lambdatop; Tk2"
                     /locus_tag="Dmel_CG32134"
                     /product="breathless, isoform B"
                     /EC_number="2.7.10.1"
                     /label=btl
                     /note="CG32134 gene product from transcript CG32134-RB; 
                     CG32134-PB; btl-PB; Fibroblast-growth-factor-receptor; 
                     devenir; no terminal cell clones-L; lethal (3) j2E11; 
                     fibroblast growth factor receptor; FGFR; fibrobast growth 
                     factor receptor; breathless; FGF receptor; fibroblast 
                     growth factor receptor 1"
                     /note="This feature has 2 segments:
                        1: 1499 .. 1834 / #cc99ff
                        2: 1920 .. 2498 / #cc99ff"
                     /db_xref="FLYBASE:FBpp0100143"
                     /db_xref="GeneID:39564"
                     /db_xref="FLYBASE:FBgn0285896"
                     /protein_id="NP_001014583.1"
                     /translation="KMVKEEHTDTDMASLVREMEVMKMIGKHINIINLLGCCSQGGPLW
                     VIVEYAPHGNLKDFLKQNRPGAPQRRSDSDGYLDDKPLISTQHLGEKELTKFAFQIARG
                     MEYLASRRCIHRDLAARNVLVSDGYVMKIADFGLARDIQDTEYYRKNTNGRLPIKWMAP
                     ESLQEKKYDSQSDVWSYGVLLWEIMTYGDQPYPHILSAEELYSYLITGQRMEKPAKCSL
                     NIYVVMRQCWHFESCARPTFAELVESFDGILQQASSNPNDAYLDLSMPMLETPPSSGDE
                     DDGSDTETFRETSPLRYQYTYKFN"
     primer_bind     1499..1535
                     /label=EKH40
                     /note="color: black; sequence: 
                     aagatggtcaaggaggagcacacggatacggacatgg; added: 2023-09-20"
     misc_feature    2371..2393
                     /label=Du et al guide 2
                     /note="color: #c0c0c0"
     primer_bind     complement(2433..2487)
                     /label=EKH41
                     /note="color: black; sequence: 
                     aggtgtactgatatctcagtggagacgtttcccggaatgtttcggtgtcggagcc; 
                     added: 2023-09-20"
     misc_feature    2453..2475
                     /label=Finder guide 1
                     /note="color: #c0c0c0"
     misc_feature    2464..2486
                     /label=mutated guide site
                     /note="color: #ff99cc"
     misc_feature    2466..2487
                     /label=Du et al guide 1
                     /note="color: #c0c0c0"
     primer_bind     2473..2532
                     /label=EKH46
                     /note="color: black; sequence: 
                     gatatcagtacacctataagtttaatGGCTCtGGtTCTCCACCTCCCGTCCCTAATCCC
                     G; added: 2023-09-20"
     CDS             2499..2807
                     /codon_start=1
                     /label=3xCD3epsilon_gblock
                     /note="color: #993366"
                     /translation="GSGSPPPVPNPDYEPIRKGQRDLYSGLNQRRIGSGSGPPPVPNPD
                     YEPIRKGQRDLYSGLNQRRIGSGSGPPPVPNPDYEPIRKGQRDLYSGLNQRRIGSGSG"
     CDS             2511..2594
                     /codon_start=1
                     /label=CD3-epsilon
                     /note="color: #ffcc00"
                     /translation="PPPVPNPDYEPIRKGQRDLYSGLNQRRI"
     CDS             2610..2693
                     /codon_start=1
                     /label=CD3-epsilon
                     /note="color: #ffcc00"
                     /translation="PPPVPNPDYEPIRKGQRDLYSGLNQRRI"
     CDS             2709..2792
                     /codon_start=1
                     /label=CD3-epsilon
                     /note="color: #ffcc00"
                     /translation="PPPVPNPDYEPIRKGQRDLYSGLNQRRI"
     primer_bind     complement(2746..2822)
                     /label=PEF071
                     /note="color: black; sequence: 
                     agtagctccgcttccGCCAGAACCagatCCTATTCGTCGTTGGTTCAATCCGCTGTAAA
                     GATCGCGTTGTCCCTTGC; added: 2021-12-06"
     primer_bind     complement(2775..2822)
                     /label=EKH_14
                     /note="color: black; sequence: 
                     agtagctccgcttccGCCAGAACCagatCCTATTCGTCGTTGGTTCAA; added: 
                     2022-01-13"
     misc_feature    2808..2873
                     /label=2A
                     /note="color: #00ffff"
     primer_bind     2808..2830
                     /label=EKH_15
                     /note="color: black; sequence: ggaagcggagctactaacttcag; 
                     added: 2022-01-13"
     primer_bind     complement(2843..2873)
                     /label=PEF215/EKH36
                     /note="color: black; sequence: 
                     aggtccagggttctcctccacgtctccagcc; added: 2023-09-20"
     primer_bind     2859..2904
                     /label=PEF216/EKH38
                     /note="color: black; sequence: 
                     gagaaccctggacctatggtgagcaagggcgaggcagtgatcaagg; added: 
                     2022-10-27"
     CDS             2874..3569
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /note="color: #ff0000"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3007..3025)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
                     /note="color: black; sequence: TTGGTCACCTTCAGCTTGG"
     primer_bind     3275..3294
                     /label=mCherry-F
                     /note="mCherry, forward primer"
                     /note="color: black; sequence: CCCCGTAATGCAGAAGAAGA"
     primer_bind     complement(3541..3584)
                     /label=PEF140/EKH39
                     /note="color: black; sequence: 
                     TGGAGAGCCGGAGCCcttgtacagctcgtccatgccgccggtgg; added: 
                     2022-06-22"
     primer_bind     3570..3592
                     /label=PEF1/EKH37
                     /note="color: black; sequence: GGCTCCGGCTCTCCAGACCCCGC; 
                     added: 2023-09-20"
     CDS             3582..4355
                     /codon_start=1
                     /product="TANDEM SH2 DOMAIN FROM ELLIOT DINE'S ZAP70"
                     /label=tandem SH2
                     /note="color: #99cc00"
                     /translation="PDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGY
                     VLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRP
                     SGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWY
                     HSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCI
                     PEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSA"
     CDS             3606..3884
                     /codon_start=1
                     /label=SH2 1
                     /note="color: #993366"
                     /translation="FFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDV
                     RFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPC"
     CDS             4065..4340
                     /codon_start=1
                     /label=SH2 2
                     /note="color: #993366"
                     /translation="WYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKT
                     VYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEAC"
     misc_feature    4359..4362
                     /label=TTAA
                     /note="color: #ff6600"
     misc_feature    complement(4363..4600)
                     /label=3' Transposon End
                     /note="color: #93949d; direction: LEFT"
     misc_feature    4601..4806
                     /label=3xP3-hsp70pro
                     /note="color: #0c71ff"
     primer_bind     complement(4648..4689)
                     /label=PEF180
                     /note="color: black; sequence: 
                     TACTCCGGCGGTCGAGGGTTCGAAATCGATAAGCTTGGATCC; added: 
                     2022-09-06"
     misc_feature    4826..5503
                     /label=dsRed2
                     /note="color: #ff0d1b"
     primer_bind     complement(4956..4974)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
                     /note="color: black; sequence: TTGGTCACCTTCAGCTTGG"
     primer_bind     5224..5243
                     /label=mCherry-F
                     /note="mCherry, forward primer"
                     /note="color: black; sequence: CCCCGTAATGCAGAAGAAGA"
     misc_feature    5504..5745
                     /label=SV40polyA
                     /note="color: #59d400"
     misc_feature    5746..6054
                     /label=5' Transposon End
                     /note="color: #93949d"
     primer_bind     complement(6019..6073)
                     /label=EKH47
                     /note="color: black; sequence: 
                     tccttatactacgaaTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGC; 
                     added: 2023-09-20"
     misc_feature    6055..6058
                     /label=TTAA
                     /note="color: #ff6600"
     misc_feature    6059..7058
                     /label=3' homology
                     /note="color: #ff6600"
     gene            6059..6432
                     /map="70D2-70D2"
                     /gene="btl"
                     /gene_synonym="0844/01; Btl; BTL; BTL/FGFR2; CG32134;
                     CG6714; CT20816; D-FGFR; dev; DFGF-R1; dFGFR; Dfr-2; DFR2; 
                     Dmel\CG32134; DmHD-311; dtk2; Dtk2; fgf-r; Fgf-r; Fgfr; 
                     FGFR; FGFR1; HD-311; l(3)00208; l(3)j2E11; lambdatop; Tk2"
                     /locus_tag="Dmel_CG32134"
                     /label=btl
                     /note="breathless"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="FLYBASE:FBgn0285896"
                     /db_xref="GeneID:39564"
     mRNA            6059..6432
                     /gene="btl"
                     /gene_synonym="0844/01; Btl; BTL; BTL/FGFR2; CG32134;
                     CG6714; CT20816; D-FGFR; dev; DFGF-R1; dFGFR; Dfr-2; DFR2; 
                     Dmel\CG32134; DmHD-311; dtk2; Dtk2; fgf-r; Fgf-r; Fgfr; 
                     FGFR; FGFR1; HD-311; l(3)00208; l(3)j2E11; lambdatop; Tk2"
                     /locus_tag="Dmel_CG32134"
                     /product="breathless, transcript variant A"
                     /label=btl mRNA
                     /note="btl-RA; Dmel\btl-RA; CG32134-RA; Dmel\CG32134-RA"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="FLYBASE:FBtr0075778"
                     /db_xref="GeneID:39564"
                     /db_xref="FLYBASE:FBgn0285896"
                     /transcript_id="NM_168577.3"
     mRNA            6059..6432
                     /gene="btl"
                     /gene_synonym="0844/01; Btl; BTL; BTL/FGFR2; CG32134;
                     CG6714; CT20816; D-FGFR; dev; DFGF-R1; dFGFR; Dfr-2; DFR2; 
                     Dmel\CG32134; DmHD-311; dtk2; Dtk2; fgf-r; Fgf-r; Fgfr; 
                     FGFR; FGFR1; HD-311; l(3)00208; l(3)j2E11; lambdatop; Tk2"
                     /locus_tag="Dmel_CG32134"
                     /product="breathless, transcript variant B"
                     /label=btl mRNA
                     /note="btl-RB; Dmel\btl-RB; CG32134-RB; Dmel\CG32134-RB"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="FLYBASE:FBtr0100676"
                     /db_xref="GeneID:39564"
                     /db_xref="FLYBASE:FBgn0285896"
                     /transcript_id="NM_001014583.2"
     primer_bind     6059..6116
                     /label=EKH42
                     /note="color: black; sequence: 
                     ttcgtagtataaggagaccaaaaagaattccaacgagtcaatcagatcccatcgaagc;
                      added: 2023-09-20"
     gene            6818..7058
                     /map="70D2-70D2"
                     /gene="CG8100"
                     /gene_synonym="Dmel\CG8100; lincRNA.S5087"
                     /locus_tag="Dmel_CG8100"
                     /label=CG8100
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="FLYBASE:FBgn0036410"
                     /db_xref="GeneID:39565"
     mRNA            6818..7058
                     /gene="CG8100"
                     /gene_synonym="Dmel\CG8100; lincRNA.S5087"
                     /locus_tag="Dmel_CG8100"
                     /product="uncharacterized protein, transcript variant C"
                     /label=CG8100 mRNA
                     /note="CG8100-RC; Dmel\CG8100-RC"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="FLYBASE:FBtr0347505"
                     /db_xref="GeneID:39565"
                     /db_xref="FLYBASE:FBgn0036410"
                     /transcript_id="NM_001316450.1"
     mRNA            6818..7058
                     /gene="CG8100"
                     /gene_synonym="Dmel\CG8100; lincRNA.S5087"
                     /locus_tag="Dmel_CG8100"
                     /product="uncharacterized protein, transcript variant B"
                     /label=CG8100 mRNA
                     /note="CG8100-RB; Dmel\CG8100-RB"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="FLYBASE:FBtr0334054"
                     /db_xref="GeneID:39565"
                     /db_xref="FLYBASE:FBgn0036410"
                     /transcript_id="NM_001274896.1"
     mRNA            6818..7058
                     /gene="CG8100"
                     /gene_synonym="Dmel\CG8100; lincRNA.S5087"
                     /locus_tag="Dmel_CG8100"
                     /product="uncharacterized protein, transcript variant A"
                     /label=CG8100 mRNA
                     /note="CG8100-RA; Dmel\CG8100-RA"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="FLYBASE:FBtr0075779"
                     /db_xref="GeneID:39565"
                     /db_xref="FLYBASE:FBgn0036410"
                     /transcript_id="NM_140436.3"
     CDS             6831..7058
                     /codon_start=1
                     /gene="CG8100"
                     /gene_synonym="Dmel\CG8100; lincRNA.S5087"
                     /locus_tag="Dmel_CG8100"
                     /product="uncharacterized protein, isoform A"
                     /label=CG8100
                     /note="CG8100 gene product from transcript CG8100-RA;
                     CG8100-PA"
                     /note="color: #993366"
                     /db_xref="FLYBASE:FBpp0075521"
                     /db_xref="GeneID:39565"
                     /db_xref="FLYBASE:FBgn0036410"
                     /protein_id="NP_648693.2"
                     /translation="MLRLTFVCLCFLVLQNRTESFGSPWKYFGSSNVEVNYNQRQDRQY
                     FLLNLLIQVHKPLLHEELITMGSQLNDDPDS"
     CDS             6831..7058
                     /codon_start=1
                     /gene="CG8100"
                     /gene_synonym="Dmel\CG8100; lincRNA.S5087"
                     /locus_tag="Dmel_CG8100"
                     /product="uncharacterized protein, isoform B"
                     /label=CG8100
                     /note="CG8100 gene product from transcript CG8100-RB;
                     CG8100-PB"
                     /note="color: #993366"
                     /db_xref="FLYBASE:FBpp0306180"
                     /db_xref="GeneID:39565"
                     /db_xref="FLYBASE:FBgn0036410"
                     /protein_id="NP_001261825.1"
                     /translation="MLRLTFVCLCFLVLQNRTESFGSPWKYFGSSNVEVNYNQRQDRQY
                     FLLNLLIQVHKPLLHEELITMGSQLNDDPDS"
     CDS             6831..7058
                     /codon_start=1
                     /gene="CG8100"
                     /gene_synonym="Dmel\CG8100; lincRNA.S5087"
                     /locus_tag="Dmel_CG8100"
                     /product="uncharacterized protein, isoform C"
                     /label=CG8100
                     /note="CG8100 gene product from transcript CG8100-RC;
                     CG8100-PC"
                     /note="color: #993366"
                     /db_xref="FLYBASE:FBpp0312579"
                     /db_xref="GeneID:39565"
                     /db_xref="FLYBASE:FBgn0036410"
                     /protein_id="NP_001303379.1"
                     /translation="MLRLTFVCLCFLVLQNRTESFGSPWKYFGSSNVEVNYNQRQDRQY
                     FLLNLLIQVHKPLLHEELITMGSQLNDDPDS"
     primer_bind     complement(7010..7058)
                     /label=EKH43
                     /note="color: black; sequence: 
                     gctgtcaggatcatcgttaagttggctccccattgtaatgagttcctcg; added: 
                     2023-09-20"
     primer_bind     7044..7083
                     /label=EKH45
                     /note="color: black; sequence: 
                     gatgatcctgacagcgGAAGAGCcgtcGCTCTTCCccgGG; added: 
                     2023-09-20"
     misc_feature    complement(7060..7066)
                     /label=SapI site
                     /label=SapI site(1)
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    complement(7071..7077)
                     /label=SapI site
                     /note="color: #ff8000; direction: LEFT"
     primer_bind     complement(7130..7147)
                     /label=PEF179
                     /note="color: black; sequence: 
                     CATCGGTGATGTCGTATAAGAGACGTATAGGAGACCTATAGTGTCTTCGG; added: 
                     2022-09-06"
     misc_feature    complement(7200..7219)
                     /label=855
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    7531..8379
                     /product="AmpR"
                     /label=AmpR
                     /note="color: #cccccc"
ORIGIN
        1 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgcgc
       61 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      121 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      181 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      241 gataccaaat actgttcttc tagtgtagcc gtagttagcc caccacttca agaactctgt
      301 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      361 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      421 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      481 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      541 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      601 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      661 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      721 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      781 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      841 gaccgagcgc agcgagtcag tgagcgagga agcggaaggc gagagtaggg aactgccagg
      901 catcaaacta agcagaaggc ccctgacgga tggccttttt gcgtttctac aaactctttc
      961 tgtgttgtaa aacgacggcc agtcttaagc tcgggccccc tgggcggttc tgataacgag
     1021 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag
     1081 tttagggaaa gagcatttgt cagaatattt aagggcgcct gtcactttgc ttgatatatg
     1141 agaattattt aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct
     1201 ttttcgattg atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat
     1261 atacaatatt tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg
     1321 gaaaatcagt ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa
     1381 actataagat gttatcagta tttattatgc atttagaata cgtactcagc gtctgggttc
     1441 cccatcggtg atgtcgtata agagacgtat aggagaccta tagtgtcttc ggggccgaaa
     1501 gatggtcaag gaggagcaca cggatacgga catggccagt ttggtgcgtg aaatggaggt
     1561 gatgaaaatg attggcaagc acataaacat catcaatctg ctaggctgct gcagccaggg
     1621 cggtcctctg tgggtgatcg tggagtatgc tccacacgga aacctcaagg acttcctcaa
     1681 gcagaatcga cctggtgctc cacagcgacg gagtgacagt gatggctatc tagatgacaa
     1741 gccactgatt tccactcaac atcttggcga aaaggagctc accaagtttg catttcaaat
     1801 cgctcgagga atggagtacc ttgcttcaag acgggtaagt tcatccaaga atgtctggct
     1861 tttaaaacct cccctcccag aactatgtat aaagatactt ataactctta ctctttcagt
     1921 gcattcatcg cgatctggct gcccgtaatg tccttgtaag cgatggatac gttatgaaga
     1981 tcgccgattt tggtctggcg agggatatcc aagatacgga gtactaccga aaaaatacca
     2041 atggacggtt gcccattaag tggatggcgc ccgagtcgct gcaggagaag aagtacgact
     2101 cacagagcga cgtctggagc tatggtgttc tgctgtggga gatcatgacc tacggggatc
     2161 aaccataccc gcacatactg tctgccgagg agctctacag ctacctgatt acgggtcagc
     2221 gcatggagaa accggccaag tgctccctga acatctacgt ggtaatgcga cagtgctggc
     2281 actttgagtc ctgtgcacga cccacgttcg cggagctggt agagagtttc gatgggatcc
     2341 tgcagcaggc tagtagcaat ccaaacgatg cctatctgga cctttcgatg ccgatgctgg
     2401 agacgccgcc ctcatcgggg gatgaggatg atggctccga caccgaaaca ttccgggaaa
     2461 cgtctccact gagatatcag tacacctata agtttaatgg ctctggttct ccacctcccg
     2521 tccctaatcc cgattatgag cccattcgga aaggtcaaag agatttgtat agtggcctca
     2581 atcaaagaag aatcgggtca ggttccggcc cgccgccagt cccaaacccc gactatgaac
     2641 caatcaggaa aggccaacgg gatttgtact caggattgaa tcagcgcagg ataggttccg
     2701 gctccgggcc accacccgtc cctaacccag attacgaacc catacgcaag ggacaacgcg
     2761 atctttacag cggattgaac caacgacgaa taggatctgg ttctggcgga agcggagcta
     2821 ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga cctatggtga
     2881 gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca
     2941 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     3001 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt
     3061 cccctcagtt catgtacggc tccagggcct tcatcaagca ccccgccgac atccccgact
     3121 actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3181 gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg
     3241 tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg
     3301 gctgggaagc gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta
     3361 agatggccct gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca
     3421 aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca
     3481 cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact
     3541 ccaccggcgg catggacgag ctgtacaagg gctccggctc tccagacccc gcggcgcacc
     3601 tgcccttctt ctacggcagc atctcgcgtg ccgaggccga ggagcacctg aagctggcgg
     3661 gcatggcgga cgggctcttc ctgctgcgcc agtgcctgcg ctcgctgggc ggctatgtgc
     3721 tgtcgctcgt gcacgatgtg cgcttccacc actttcccat cgagcgccag ctcaacggca
     3781 cctacgccat tgccggcggc aaagcgcact gtggaccggc agagctctgc gagttctact
     3841 cgcgcgaccc cgacgggctg ccctgcaacc tgcgcaagcc gtgcaaccgg ccgtcgggcc
     3901 tcgagccgca gccgggggtc ttcgactgcc tgcgagacgc catggtgcgt gactacgtgc
     3961 gccagacgtg gaagctggag ggcgaggccc tggagcaggc catcatcagc caggccccgc
     4021 aggtggagaa gctcattgct acgacggccc acgagcggat gccctggtac cacagcagcc
     4081 tgacgcgtga ggaggccgag cgcaaacttt actctggggc gcagaccgac ggcaagttcc
     4141 tgctgaggcc gcggaaggag cagggcacat acgccctgtc cctcatctat gggaagacgg
     4201 tgtaccacta cctcatcagc caagacaagg cgggcaagta ctgcattccc gagggcacca
     4261 agtttgacac gctctggcag ctggtggagt atctgaagct gaaggcggac gggctcatct
     4321 actgcctgaa ggaggcctgc cccaacagca gtgcctgatt aaccctagaa agataatcat
     4381 attgtgacgt acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg
     4441 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
     4501 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac
     4561 aaaaactcaa aatttcttct ataaagtaac aaaactttta ggatctaatt caattagaga
     4621 ctaattcaat tagagctaat tcaattagga tccaagctta tcgatttcga accctcgacc
     4681 gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa
     4741 gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac
     4801 aatcggctcg aagccggtcg ccaccatggc ctcctccgag gacgtcatca aggagttcat
     4861 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     4921 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     4981 ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta
     5041 cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa
     5101 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     5161 cctccaggac ggctccttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga
     5221 cggccccgta atgcagaaga agactatggg ctgggaggcg tccaccgagc gcctgtaccc
     5281 ccgcgacggc gtgctgaagg gcgagatcca caaggccctg aagctgaagg acggcggcca
     5341 ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta
     5401 ctactacgtg gactccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     5461 gcagtacgag cgcgccgagg gccgccacca cctgttcctg tagcggccgc gactctagat
     5521 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     5581 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     5641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5701 actgcattct agttgtggtt tgtccaaact catcaatgta tcttagatat ctataacaag
     5761 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     5821 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     5881 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     5941 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     6001 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatt
     6061 cgtagtataa ggagaccaaa aagaattcca acgagtcaat cagatcccat cgaagcatcg
     6121 ccaaccatca tctcatccca gaaagtgcca aagaaaagat cgcagaagca gaaacatcag
     6181 aacaagtttt tcaagctcta aacttcgcat tgtttgaatt ggtgtattta ataaataata
     6241 aataataaat ccgatatcag atatccaatg ctacacgatt ttggcttaat cacaaagact
     6301 taacttgcac ttagcaatct cattctttag ttttacaaag ttcatatgta tctttgtata
     6361 tctacaatgt tttgttctgg gtctttgggc tacatttatt tcatagtgtt tcttgttgaa
     6421 ccaataaaaa cgaatttgtt ttactgtaac taagaatttt gatcaccaca aataatgctc
     6481 actttctccc aagaaccgct cataatcaac cgcagcgata accaagttca gtgtgtttta
     6541 tatgaaaaga aaacaagaga gcaagctagc tgtggcaatt ccaagcttat atacccttgc
     6601 agtaattcta tatttatacg aaaaagaaga ctttatgata aaaaagcacg gatctatgat
     6661 atcttttctt attaccggga atatatagaa gtttttaaga aaagtggcag gcatttatat
     6721 actagtttac gtggaaatca atgctccctt agtaagggta tgaaacaaaa caatcgcatt
     6781 taagaatata tgcagaagtc agttcatgtc atcgatcagt ctgcgtcaaa atgttaagat
     6841 taacgtttgt gtgcctttgt tttctcgttc ttcaaaatag aacagaaagt tttggcagtc
     6901 cttggaagta tttcggatca tcaaatgtag aagtgaatta taatcaaagg caagatcgtc
     6961 agtattttct cctgaatttg ctcattcaag tccacaaacc tttgcttcac gaggaactca
     7021 ttacaatggg gagccaactt aacgatgatc ctgacagcgg aagagccgtc gctcttcccc
     7081 gggaaaagaa gaccatatac gtctcctata ggtctcgtat actgcttcct atacgacatc
     7141 accgatgggg aacacgaagc gatccagccc ccctaattag cccgggcgaa aggcccagtc
     7201 tttcgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcatggggag
     7261 tccccacact accatcggcg ctacggcgtt tcacttctga gttcggcatg gggtcaggtg
     7321 ggaccaccgc gctactgccg ccaggcaaac aaggggtgtt atgagccata ttcaggtata
     7381 aatgggctcg cgataatgtt cagaattggt taattggttg taacactgac ccctatttgt
     7441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7501 cttcaataat attgaaaaag gaagaatatg agtattcaac atttccgtgt cgcccttatt
     7561 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7621 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7681 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7741 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7801 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7861 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7921 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7981 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8041 ccaaacgacg agcgtgacac cacgatgcct gtagcgatgg caacaacgtt gcgcaaacta
     8101 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8161 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8221 aaatccggag ccggtgagcg tggttctcgc ggtatcatcg cagcgctggg gccagatggt
     8281 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8341 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaaaa gcagagcatt
     8401 acg
//
