LOCUS       Exported                6673 bp ds-DNA     circular SYN 02-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      541..550
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             547..1236
                     /codon_start=1
                     /label=mScarlet-I
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDEL"
     CDS             1243..1767
                     /codon_start=1
                     /label=mRIMS2 RBD
                     /translation="MSAPLGPRGRPAPTPAASQPPPQPEMPDLSHLTEEERKIILAVMD
                     RQKKEEEKEQSVLKKLHQQFEMYKEQVKKMGEESQQQQEQKGDAPTCGICHKTKFADGC
                     GHNCSYCQTKFCARCGGRVSLRSNKVMWVCNLCRKQQEILTKSGAWFYNSGSNTLQQPD
                     QKVPRGLRNEEA"
     CDS             1813..2508
                     /codon_start=1
                     /label=mNeongreen
                     /translation="SKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEEL
                     NLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGA
                     SLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTF
                     KWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKA
                     FTDVMGMDEL"
     regulatory      2062..2071
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2554..3216
                     /codon_start=1
                     /label=Rab3Q81L
                     /translation="MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADD
                     SFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGLERYRTITTAYYRGAMGFILMYDI
                     TNEESFNAVQDWSTQIKTYSWDNAQVLLVGNKCDMEDERVVSSERGRQLADHLGFEFFE
                     ASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQGPQLTDQQAPPHQDCAC"
     misc_feature    3221..3277
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    3401..3522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3529..3984)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4011..4115
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4117..4474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4117..4308
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      4325..4460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4509..5303
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4509..5303
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5535..5582
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5911..6499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 gccaccatgg tgagcaaggg cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac
      601 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      661 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttctcc
      721 tgggacatcc tgtcccctca gttcatgtac ggctccaggg ccttcatcaa gcaccccgcc
      781 gacatccccg actactataa gcagtccttc cccgagggct tcaagtggga gcgcgtgatg
      841 aacttcgagg acggcggcgc cgtgaccgtg acccaggaca cctccctgga ggacggcacc
      901 ctgatctaca aggtgaagct ccgcggcacc aacttccctc ctgacggccc cgtaatgcag
      961 aagaagacaa tgggctggga agcgtccacc gagcggttgt accccgagga cggcgtgctg
     1021 aagggcgaca ttaagatggc cctgcgcctg aaggacggcg gccgctacct ggcggacttc
     1081 aagaccacct acaaggccaa gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc
     1141 aagttggaca tcacctccca caacgaggac tacaccgtgg tggaacagta cgaacgctcc
     1201 gagggccgcc actccaccgg cggcatggac gagctgggcg gcatgtctgc tccgttaggt
     1261 ccccgtggtc gtccggctcc cactccggct gcatctcaac ctcctccgca gcccgagatg
     1321 ccggacctca gccacctcac ggaagaggag aggaaaatca tcctggctgt catggatcgt
     1381 cagaagaaag aagaggagaa ggagcagtcc gtgctcaaga agctgcacca acaatttgaa
     1441 atgtataagg agcaagtcaa gaagatggga gaggaatcgc agcagcagca agagcagaag
     1501 ggtgatgccc cgacctgtgg catctgccac aagacaaaat ttgcagatgg atgcggccat
     1561 aattgttcct attgccaaac caagttctgt gctcgatgtg gaggtcgagt gtctttacgc
     1621 tcaaacaagg ttatgtgggt gtgtaatttg tgccgaaaac aacaagaaat cctcactaaa
     1681 tcaggagcat ggttttataa tagtgggtct aacacactgc agcaacctga tcaaaaggtt
     1741 cctcgagggc ttcgaaatga ggaagccggc ggaggcggtg gttcaggggg agggggatct
     1801 ggaggtggtg gcagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag
     1861 ttacacatct ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc
     1921 aatccaaatg atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc
     1981 tccccctgga ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct
     2041 gacgggatgt cgcctttcca ggccgccatg gtagatggct ccggatacca agtccatcgc
     2101 acaatgcagt ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga
     2161 agccacatca aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg
     2221 atgaccaact cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac
     2281 aaaaccatca tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg
     2341 agcactgcgc ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac
     2401 cagccgatgt acgtgttccg taagacggag ctcaagcact ccaagaccga gctcaacttc
     2461 aaggagtggc aaaaggcctt taccgatgtg atgggcatgg acgagctggg cggaggcggt
     2521 ggttcagggg gagggggatc tggaggtggt ggcatggcct cagccacaga ctctcgatat
     2581 gggcagaagg agtcctcaga ccagaacttc gactatatgt tcaagatcct gatcattggt
     2641 aacagcagtg tgggcaaaac ctcattcctt ttccgctacg cagacgactc ttttactcca
     2701 gcctttgtca gcactgtggg catagacttc aaggtcaaaa ccatctaccg caatgacaag
     2761 aggatcaagc tgcagatctg ggacacagca ggactggagc ggtaccgaac catcaccaca
     2821 gcctactacc ggggcgccat gggcttcatt ctaatgtatg acatcaccaa tgaggagtcc
     2881 ttcaatgcag tgcaggactg gtccactcag atcaaaactt actcatggga caatgcccag
     2941 gtgctgctgg tggggaacaa gtgcgacatg gaggacgagc gagtggtgtc ctcagaacga
     3001 ggccggcagc tggccgacca cctgggcttt gagttctttg aggccagcgc caaggacaac
     3061 attaatgtca agcagacctt tgaacgtctg gtggacgtga tctgtgagaa gatgtcggag
     3121 tccctagata ctgcagaccc tgcagtcacg ggtgccaagc agggcccaca gctcaccgac
     3181 cagcaggcac cacctcatca ggattgcgcc tgctaaactc agatctcgag ctcaagcttc
     3241 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
     3301 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3361 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3481 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     3541 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3601 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3661 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3721 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3781 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3841 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3901 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3961 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4021 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4081 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     4141 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4201 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4321 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4381 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4441 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     4501 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4561 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4621 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4681 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4741 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4801 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4861 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4921 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4981 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     5041 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5101 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5161 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5221 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5281 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5341 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5401 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5461 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5521 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     5581 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5641 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5701 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     5761 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5821 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6301 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6481 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6541 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6601 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
     6661 atatggagtt ccg
//
