LOCUS       Exported                1247 bp ds-DNA     linear   UNA 30-APR-2024
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     gap             1..17
                     /estimated_length=17
     regulatory      67..76
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             146..169
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             188..211
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             212..235
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     gap             1125..1145
                     /estimated_length=21
     gap             1147..1160
                     /estimated_length=14
     gap             1165..1197
                     /estimated_length=33
     gap             1199..1213
                     /estimated_length=15
ORIGIN
        1 nnnnnnnnnn nnnnnnnagn nnnnnnggtt nngtgaaccg tcagannccg ctagcgctna
       61 ccggtcgcca ccatgggcag cgcatggagt catcctcaat tncgagaaag gtggaggttc
      121 tggcggtgga tcgggaggtt cagcgtggag ccacccgcag ttcgaaaaat ccggaggtgc
      181 ttctggtctg gaagttctgt tccagggacc cgattacaag gatgacgacg ataagtccgg
      241 actcagatct cgagctcaag cttcgaattc tgcagtcgac atggcctcag ccacagactc
      301 tcgatatggg cagaaggagt cctcagacca gaacttcgac tatatgttca agatcctgat
      361 cattggtaac agcagtgtgg gcaaaacctc attccttttc cgctacgcag acgactcttt
      421 tactccagcc tttgtcagca ctgtgggcat agacttcaag gtcaaaacca tctaccgcaa
      481 tgacaagagg atcaagctgc agatctggga cacagcagga caagagcggt accgaaccat
      541 caccacagcc tactaccggg gcgccatggg cttcattcta atgtatgaca tcaccaatga
      601 ggagtccttc aatgcagtgc aggactggtc cactcagatc aaaacttact catgggacaa
      661 tgcccaggtg ctgctggtgg ggaacaagtg cgacatggag gacgagcgag tggtgtcctc
      721 agaacgaggc cggcagctgg ccgaccacct gggctttgag ttctttgagg ccagcgccaa
      781 ggacaacatt aatgtcaagc aganctttga acgtctggtg gacgtgatct gtgagaagat
      841 gtcggantcc ctagatactg cagaccctgc agtcacgggt gccaagcagg gcccacagct
      901 caccgaccag cnnnaccacc tcatcnggan tgcgcctgct gaggatccac cggatctaga
      961 nactgatcat aatcagccnt accacatttg tagaggtttt acttgctnna aaanctccca
     1021 nacncccctg anntgaaaca tnanngangn nnntnnntnn ntntnntgna gctnnannnn
     1081 nnnaannann ncatcnnnan tncnnnnnna ncntttttnn nngcnnnnnn nnnnnnnnnn
     1141 nnnnncnnnn nnnnnnnnnn cnnannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncnn
     1201 nnnnnnnnnn nnncnnannn nnnnnncnnn nnannnnnaa nnnnnnn
//
