LOCUS       Exported                1016 bp ds-DNA     linear   UNA 25-AUG-2021
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     regulatory      155..164
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             161..871
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 cctgntcgtg tgcctgggtg cgctggccga ggcgtacccn tccaagccgg acaacccggg
       61 ngaggacgca ccagcggagg acatggccag atantantcg gcgntgngac antacatcaa
      121 cntcatcacc aggcagagat atggaaaacc ggtcgccacc atggtgtcta agggcgaaga
      181 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
      241 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
      301 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
      361 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
      421 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
      481 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
      541 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
      601 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
      661 gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc acatatagat ccaagaaacc
      721 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
      781 ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
      841 cctccctagc aaactggggc acaagcttaa ttaagcggcc gcgactctag atcataatca
      901 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      961 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gttatgnagc ttnatn
//
