LOCUS       Exported                 997 bp ds-DNA     linear   UNA 04-AUG-2021
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Aug 6, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             644..714
                     /codon_start=1
                     /label=EGFP
                     /translation="RDHMVLLEFVTAAGITLGMDELY"
     CDS             728..748
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             755..844
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 ttcacccggg ggttggttgc cccantcntg gttnaagctg ggacggggga acgtaaaanc
       61 nggccacaag ttncagcgtn ttccggcgaa gggcgaaggg ncgatgccac ntacgggcaa
      121 ngctgaccct gaagtttcat ctgcaccacc ggcaagctgc ccgtgcccct ggcccaccct
      181 cgtgaccacc ctgacntacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      241 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      301 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      361 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      421 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      481 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      541 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      601 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      661 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggagg
      721 ttctggtgag aatctctact tccaatctgg tgctagcgca tggagtcatc ctcaattcga
      781 gaaaggtgga ggttctggcg gtggatcggg aggttcagcg tggagccacc cgcagttcga
      841 aaaataaaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
      901 gttttacttg ctttaaaaaa cctcccacac ctccccctga agcctgaaac cataaaatga
      961 atgcaattgt tgttgttaac tgttatgcag ctnatta
//
