LOCUS       Exported                3412 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     A/B/C/D region defined in Besnard et al. 1991 JBC 266: 18877-18883. 
            Cloning: Fragment Bsu36i-SmaI from the plasmid#2478 cloned into the 
            #1789 digested Bsu36i-SmaI Comment:
            Deleted fragment 497-519 GAATTCGAGCTCGGTACC between ATTL1 and 
            promoter from original map #2481 and #1789. No more EcoRI ApoI SacI 
            and second KpnI Also other mismatch into the G1B3 promoter after 
            sequencing #3286.
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      106..149
                     /note="rrnB T1 transcription termination sequence"
     terminator      281..308
                     /note="rrnB T2 transcription termination sequence"
     N_region        complement(358..457)
                     /label=attL1
                     /note="attL1"
     primer_bind     join(396..420,1044..1048,1480..1612)
                     /label=pENTR-1F
                     /note="pENTR-1F"
     promoter        503..1004
                     /label=GFA-ABC1D
     misc_feature    503..646
                     /label=A region (-1757/-1604)
     promoter        648..771
                     /label=B3 element
                     /label=B3 enhancer
     misc_feature    648..771
                     /label=B region (-1612/-1489)
     misc_feature    772..1004
                     /label=C1 region
     misc_feature    1005..1084
                     /label=D region (-132/-57)
     primer_bind     1084..1476
                     /label=B3 enhancer
     promoter        1089..1212
                     /label=B3 element
                     /label=B3 enhancer
     promoter        1221..1344
                     /label=B3 element
                     /label=B3 enhancer
     promoter        1353..1476
                     /label=B3 element
                     /label=B3 enhancer
     misc_feature    1477..1584
                     /label=Basal promoter +47
     N_region        1641..1740
                     /label=attL2
                     /note="attL2"
     primer_bind     1764..1787
                     /label=pENTR-2R
                     /note="pENTR-2R"
     gene            1863..2672
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2736..3409
                     /label=pUC origin
                     /note="pUC origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc
      481 gactggatcc ggtaccagat ctaacatatc ctggtgtgga gtaggggacg ctgctctgac
      541 agaggctcgg gggcctgagc tggctctgtg agctggggag gaggcagaca gccaggcctt
      601 gtctgcaagc agacctggca gcattgggct ggccgccccc cagggcctcc tcttcatgcc
      661 cagtgaatga ctcaccttgg cacagacaca atgttcgggg tgggcacagt gcctgcttcc
      721 cgccgcaccc cagcccccct caaatgcctt ccgagaagcc cattgagcag ggggcttgca
      781 ttgcacccca gcctgacagc ctggcatctt gggataaaag cagcacagcc ccctaggggc
      841 tgcccttgct gtgtggcgcc accggcggtg gagaacaagg ctctattcag cctgtgccca
      901 ggaaagggga tcaggggatg cccaggcatg gacagtgggt ggcagggggg gagaggaggg
      961 ctgtctgctt cccagaagtc caaggacaca aatgggtgag gggagagctc tccccatagc
     1021 tggggctgcg gcccaacccc ccaccccctc aggctatgcc agggggtgtt gccaggggca
     1081 cccgatcttc ctcttcatgc ccagtgaatg actcaccttg gcacagacac aatgttcggg
     1141 gtgggcacag tgcctgcttc ccgccgcacc ccagcccccc tcaaatgcct tccgagaagc
     1201 ccattgagca ggcccgatct tcctcttcat gcccagtgaa tgactcacct tggcacagac
     1261 acaatgttcg gggtgggcac agtgcctgct tcccgccgca ccccagcccc cctcaaatgc
     1321 cttccgagaa gcccattgag caggcccgat cttcctcttc atgcccagtg aatgactcac
     1381 cttggcacag acacaatgtt cggggtgggc acagtgcctg cttcccgccg caccccagcc
     1441 cccctcaaat gccttccgag aagcccattg agcaggcccg ggcatcgcca gtctagccca
     1501 ctccttcata aagccctcgc atcccaggag cgagcagagc cagagcaggt tggagaggag
     1561 acgcatcacc tccgctgctc gcggggatcc tctagagtcg acggatccgg ggaattcgcg
     1621 gccgcactcg agatatctag acccagcttt cttgtacaaa gttggcatta taagaaagca
     1681 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     1741 ccatccagct gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     1801 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     1861 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg
     1921 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     1981 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     2041 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     2101 cgaccatcaa gcattttatc cgtactcctg gtgatgcatg gttactcacc actgcgatcc
     2161 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     2221 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     2281 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     2341 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     2401 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     2461 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     2521 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     2581 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     2641 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacatt
     2701 attcagattg ggccccgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2881 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
