LOCUS       Exported               11726 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     BamHI site at position 2628
            XhoI site at position 3331
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     5'UTR           1..634
                     /label=5'LTR
                     /note="5' LTR"
     misc_feature    1176..1676
                     /label=RRE
                     /note="RRE"
     misc_feature    2026..2143
                     /label=cPPT
                     /note="cPPT"
     promoter        2422..4621
                     /label=Gfa2(B)3
                     /note="/Gfa2(B)3="
     misc_feature    4123..4515
                     /label=(B)3
                     /note="/(B)3="
     misc_feature    4582..4588
                     /label=RNA start +1
                     /note="/RNA start+1="
     misc_feature    4657..5470
     CDS             4670..5381
                     /codon_start=1
                     /transl_table=11
                     /gene="egfp"
                     /label=egfp
                     /note="enhanced green fluorescent protein [Split]"
                     /db_xref="PID:g1377915"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDEL"
     misc_feature    5380..5387
     misc_feature    5380..5381
     misc_feature    5484..6091
                     /label=WPRE
                     /note="WPRE"
     misc_signal     6108..6212
                     /label=miR124T
                     /note="miR124T Naldini"
     misc_feature    6285..6523
                     /label=3'LTR-SIN
                     /note="3'LTR-SIN [Split]"
     misc_feature    6285..6339
                     /label=deltaU3
                     /note="delU3"
     misc_feature    6338..6435
                     /label=R
                     /note="R"
     misc_feature    6436..6523
                     /label=U5 [Split]
                     /note="U5 [Split]"
     gene            7455..8255
                     /gene="ampicilline gene resistance"
                     /label=Ampicilline gene resistance
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag
      661 cgaaagggaa accagagctc tctcgacgca ggactcggct tgctgaagcg cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cctcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattg
     1621 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1681 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1741 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1801 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1861 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1921 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     1981 cagatccatt cgattagtgg acggatctcg acggtatcgg ttaactttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatccgat gtacgggcca
     2161 gatatacgcg ttgacattga ttattgacta gaccagcctg ggcaacatgg caagacccta
     2221 tctctacaaa aaaagttaaa aaatcagcca cgtgtggtga cacacacctg tagtcccagc
     2281 tattcaggag gctgaggtga ggggatcact taaggctggg aggttgaggc tgcagtgagt
     2341 cgtggttgcg ccactgcact ccagcctggg caacagtgag accctgtctc aaaagacaaa
     2401 aaaaaaaaaa aaaaaaaaaa gaacatatcc tggtgtggag taggggacgc tgctctgaca
     2461 gaggctcggg ggcctgagct ggctctgtga gctggggagg aggcagacag ccaggccttg
     2521 tctgcaagca gacctggcag cattgggctg gccgcccccc agggcctcct cttcatgccc
     2581 agtgaatgac tcaccttggc acagacacaa tgttcggggt gggcacagtg cctgcttccc
     2641 gccgcacccc agcccccctc aaatgccttc cgagaagccc attgagcagg gggcttgcat
     2701 tgcaccccag cctgacagcc tggcatcttg ggataaaagc agcacagccc cctaggggct
     2761 gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc tctattcagc ctgtgcccag
     2821 gaaaggggat caggggatgc ccaggcatgg acagtgggtg gcaggggggg agaggagggc
     2881 tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg ggactgggca gggttctgac
     2941 cctgtgggac cagagtggag ggcgtagatg gacctgaagt ctccagggac aacagggccc
     3001 aggtctcagg ctcctagttg ggcccagtgg ctccagcgtt tccaaaccca tccatcccca
     3061 gaggttcttc ccatctctcc aggctgatgt gtgggaactc gaggaaataa atctccagtg
     3121 ggagacggag gggtggccag ggaaacgggg cgctgcagga ataaagacga gccagcacag
     3181 ccagctcatg tgtaacggct ttgtggagct gtcaaggcct ggtctctggg agagaggcac
     3241 agggaggcca gacaaggaag gggtgacctg gagggacaga tccaggggct aaagtcctga
     3301 taaggcaaga gagtgccggc cccctcttgc cctatcagga cctccactgc cacatagagg
     3361 ccatgattga cccttagaca aagggctggt gtccaatccc agcccccagc cccagaactc
     3421 cagggaatga atgggcagag agcaggaatg tgggacatct gtgttcaagg gaaggactcc
     3481 aggagtctgc tgggaatgag gcctagtagg aaatgaggtg gcccttgagg gtacagaaca
     3541 ggttcattct tcgccaaatt cccagcacct tgcaggcact tacagctgag tgagataatg
     3601 cctgggttat gaaatcaaaa agttggaaag caggtcagag gtcatctggt acagcccttc
     3661 cttccctttt tttttttttt tttttgtgag acaaggtctc tctctgttgc ccaggctgga
     3721 gtggcgcaaa cacagctcac tgcagcctca acctactggg ctcaagcaat cctccagcct
     3781 cagcctccca aagtgctggg attacaagca tgagccaccc cactcagccc tttccttcct
     3841 ttttaattga tgcataataa ttgtaagtat tcatcatggt ccaaccaacc ctttcttgac
     3901 ccaccttcct agagagaggg tcctcttgct tcagcggtca gggccccaga cccatggtct
     3961 ggctccaggt accacctgcc tcatgcagga gttggcgtgc ccaggaagct ctgcctctgg
     4021 gcacagtgac ctcagtgggg tgaggggagc tctccccata gctgggctgc ggcccaaccc
     4081 caccccctca ggctatgcca gggggtgttg ccaggggcac ccgatcttcc tcttcatgcc
     4141 cagtgaatga ctcaccttgg cacagacaca atgttcgggg tgggcacagt gcctgcttcc
     4201 cgccgcaccc cagcccccct caaatgcctt ccgagaagcc cattgagcag gcccgatctt
     4261 cctcttcatg cccagtgaat gactcacctt ggcacagaca caatgttcgg ggtgggcaca
     4321 gtgcctgctt cccgccgcac cccagccccc ctcaaatgcc ttccgagaag cccattgagc
     4381 aggcccgatc ttcctcttca tgcccagtga atgactcacc ttggcacaga cacaatgttc
     4441 ggggtgggca cagtgcctgc ttcccgccgc accccagccc ccctcaaatg ccttccgaga
     4501 agcccattga gcaggcccgg gcatcgccag tctagcccac tccttcataa agccctcgca
     4561 tcccaggagc gagcagagcc agagcaggtt ggagaggaga cgcatcacct ccgctgctcg
     4621 cggggatcca ggcctaagct tacgcgtcct agcgctaccg gtcgccacca tggtgagcaa
     4681 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4741 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4801 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4861 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4921 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4981 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5041 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5101 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5161 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5221 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     5281 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5341 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc
     5401 tcgactagct agtagctagc tagctagtcg agctcaagct tcgaattcga tatcaagctt
     5461 atcgataccg tcgacctcga gggaattccg ataatcaacc tctggattac aaaatttgtg
     5521 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5581 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5641 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5701 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5761 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5821 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5881 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5941 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6001 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6061 ccctttgggc cgcctccccg catcgggaat tcgagctcgg tacctaatgg cattcaccgc
     6121 gtgccttaat tcgaatggca ttcaccgcgt gccttaaacg cgttggcatt caccgcgtgc
     6181 cttaaatgca ttggcattca ccgcgtgcct taaggtacct ttaagaccaa tgacttacaa
     6241 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6301 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6361 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6421 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6481 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atnctagaat taattccgtg
     6541 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     6601 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     6661 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     6721 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     6781 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     6841 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     6901 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     6961 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     7021 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgat atggtgcact
     7081 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     7141 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7201 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     7261 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7321 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7381 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7441 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7501 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7561 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7621 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7681 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7741 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7801 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7861 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7921 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7981 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8041 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8101 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8161 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8221 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8281 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8341 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8401 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     8641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9181 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9241 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9301 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     9361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     9481 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9541 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     9601 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     9661 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     9721 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     9781 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     9841 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     9901 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     9961 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
    10021 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
    10081 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
    10141 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
    10201 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
    10261 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
    10321 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
    10381 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
    10441 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
    10501 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
    10561 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
    10621 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
    10681 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
    10741 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
    10801 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
    10861 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
    10921 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
    10981 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
    11041 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
    11101 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
    11161 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
    11221 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
    11281 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
    11341 ttgtagagct tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    11401 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    11461 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11521 tgtccaaact catcaatgta tcttatcatg tgtggatcaa ctggataact caagctaacc
    11581 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaaatta cctgtggttt
    11641 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    11701 taaatatgta ctacaaactt agtagt
//
