LOCUS       Exported               10569 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     BamHI site at position 2628
            XhoI site at position 3331
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     5'UTR           1..634
                     /label=5'LTR
                     /note="5' LTR"
     misc_feature    1176..1676
                     /label=RRE
                     /note="RRE"
     misc_feature    join(2026..2143,3201..3310)
                     /label=cPPT
                     /note="cPPT"
     promoter        join(2227..2769,3201..3304)
                     /label=GFA-ABC1D
                     /note="GFAP promoter of 681 bp from Dr M. Brenner 
                     corresponding to GFAP promoter sequences -1757 to -1256 / 
                     -132 to +47 [Split]"
     misc_feature    2805..3197
                     /label=(B)3
                     /note="/(B)3="
     misc_feature    3427..4251
     gene            3440..4189
                     /label=GFP*
                     /note="/GFP*="
     misc_feature    4150..4157
     misc_feature    4150..4151
     misc_feature    4328..4935
                     /label=WPRE
                     /note="WPRE"
     misc_signal     4952..5056
                     /label=miR124T
                     /note="miR124T Naldini"
     misc_feature    5129..5366
                     /label=3'LTR-SIN
                     /note="3'LTR-SIN [Split]"
     misc_feature    5129..5183
                     /label=deltaU3
                     /note="delU3"
     misc_feature    5182..5279
                     /label=R
                     /note="R"
     misc_feature    5280..5366
                     /label=U5 [Split]
                     /note="U5 [Split]"
     gene            6298..7098
                     /gene="ampicilline gene resistance"
                     /label=Ampicilline gene resistance
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag
      661 cgaaagggaa accagagctc tctcgacgca ggactcggct tgctgaagcg cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cctcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattg
     1621 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1681 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1741 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1801 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1861 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1921 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     1981 cagatccatt cgattagtgg acggatctcg acggtatcgg ttaactttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatccgat gtacgggcca
     2161 gatatacgcg ttgacattga ttattgacta ggatccggta ccgaattcga gctcggtacc
     2221 agatctaaca tatcctggtg tggagtaggg gacgctgctc tgacagaggc tcgggggcct
     2281 gagctggctc tgtgagctgg ggaggaggca gacagccagg ccttgtctgc aagcagacct
     2341 ggcagcattg ggctggccgc cccccagggc ctcctcttca tgcccagtga atgactcacc
     2401 ttggcacaga cacaatgttc ggggtgggca cagtgcctgc ttcccgccgc accccagccc
     2461 ccctcaaatg ccttccgaga agcccattga gcagggggct tgcattgcac cccagcctga
     2521 cagcctggca tcttgggata aaagcagcac agccccctag gggctgccct tgctgtgtgg
     2581 cgccaccggc ggtggagaac aaggctctat tcagcctgtg cccaggaaag gggatcaggg
     2641 gatgcccagg catggacagt gggtggcagg gggggagagg agggctgtct gcttcccaga
     2701 agtccaagga cacaaatggg tgaggggaga gctctcccca tagctgggct gcggcccaac
     2761 cccaccccct caggctatgc cagggggtgt tgccaggggc acccgatctt cctcttcatg
     2821 cccagtgaat gactcacctt ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt
     2881 cccgccgcac cccagccccc ctcaaatgcc ttccgagaag cccattgagc aggcccgatc
     2941 ttcctcttca tgcccagtga atgactcacc ttggcacaga cacaatgttc ggggtgggca
     3001 cagtgcctgc ttcccgccgc accccagccc ccctcaaatg ccttccgaga agcccattga
     3061 gcaggcccga tcttcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt
     3121 tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga
     3181 gaagcccatt gagcaggccc gggcatcgcc agtctagccc actccttcat aaagccctcg
     3241 catcccagga gcgagcagag ccagagcagg ttggagagga gacgcatcac ctccgctgct
     3301 cgcggggatc ctagtggatc ccccgggctg caggaattat cacaagtttg tacaaaaaag
     3361 caggctccac catgggaacc aattcagtcg actggatcca ggcctaagct tacgcgtcct
     3421 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3481 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3541 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3601 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     3661 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3721 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3781 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3841 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3901 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3961 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4021 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     4081 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4141 ggacgagctg tacaagtccg gactcagatc tcgactagct agtagctagc tagctagtcg
     4201 agctcaagct tcgaattcga tatcaagctt atcgataccg tcgacctcga gatatctaga
     4261 cccagctttc ttgtacaaag tggtgataat tcgatatcaa gcttatcgat accgtcgacc
     4321 tcgagggaat tccgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4381 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4441 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4501 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4561 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4621 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4681 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     4741 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4801 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4861 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4921 cccgcatcgg gaattcgagc tcggtaccta atggcattca ccgcgtgcct taattcgaat
     4981 ggcattcacc gcgtgcctta aacgcgttgg cattcaccgc gtgccttaaa tgcattggca
     5041 ttcaccgcgt gccttaaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5101 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5161 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5221 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5281 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5341 tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc
     5401 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
     5461 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
     5521 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
     5581 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
     5641 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     5701 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     5761 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     5821 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     5881 taatgcagga gtcgcataag ggagagcgtc gatatggtgc actctcagta caatctgctc
     5941 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     6001 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     6061 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     6121 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     6181 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6241 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6301 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6361 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6421 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6481 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6541 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6601 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6661 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6721 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6781 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6841 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6901 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6961 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7021 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7081 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7141 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7201 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7441 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8101 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
     8161 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8221 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8281 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8341 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8401 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8461 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
     8521 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     8581 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     8641 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     8701 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     8761 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
     8821 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     8881 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     8941 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     9001 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     9061 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     9121 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     9181 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     9241 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     9301 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
     9361 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     9421 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
     9481 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
     9541 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     9601 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     9661 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     9721 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     9781 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     9841 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     9901 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     9961 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
    10021 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
    10081 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
    10141 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga gcttttactt
    10201 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    10261 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10321 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10381 gtatcttatc atgtgtggat caactggata actcaagcta accaaaatca tcccaaactt
    10441 cccaccccat accctattac cactgccaaa ttacctgtgg tttcatttac tctaaacctg
    10501 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10561 cttagtagt
//
