LOCUS       Exported                8041 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Reu du groupe de M. During en 2001
            Sequence 1 ˆ 109 thŽorique prise sur vecteur 2343 
            pDEST-ssAAV-RFA-bGHpoly d'Alexi Demelmans 
            Enhancer immediate early gene CMV = identique vecteur AAV-CBA-hARSA 
            (Nathalie cartier) Chicken beta actin promoter (CBA) = identique 
            vecteur AAV-CBA-hARSA (Nathalie cartier) Intron chicken beta actin =
            identique vecteur AAV-CBA-hARSA (Nathalie cartier) jusqu'ˆ la 
            position 980
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     5'UTR           36..163
                     /label=5' ITR
                     /label=ITR
                     /note="/SerialCloner_Color="
                     /note="""/SerialCloner_Show=True"
                     /note="/SerialCloner_Arrow=True"""
                     /note="/SerialCloner_Protect=False"
     promoter        join(219,3866..3872)
                     /label=enhancer immediate early gene CMV
                     /note="CMV immediate early region"
     promoter        633..2832
                     /label=Gfa2(B)3
                     /note="/Gfa2(B)3="
     misc_feature    2334..2726
                     /label=(B)3
                     /note="/(B)3="
     misc_feature    2793..2799
                     /label=RNA start +1
                     /note="/RNA start+1="
     misc_feature    2852..2879
                     /label=phagemid pBluescriptII SK(+) [Split]
                     /note="phagemid pBluescriptII SK(+) [Split]"
     gene            join(2880..2890,3811..3828)
                     /label=/RFA= [Split]
                     /note="/RFA= [Split]"
     N_region        complement(2891..2907)
                     /label=attL1 [Split]
                     /note="attL1 [Split]"
     N_region        2968..3792
     CDS             2981..3692
                     /codon_start=1
                     /transl_table=11
                     /gene="egfp"
                     /label=egfp
                     /note="enhanced green fluorescent protein [Split]"
                     /db_xref="PID:g1377915"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDEL"
     N_region        3691..3698
     N_region        3691..3692
     N_region        join(3801..3828,4519..4755,4782..4949)
                     /label=attL2 [Split]
                     /note="attL2 [Split]"
     misc_feature    3811..3865
     misc_feature    3811..3865
     misc_feature    3811..3828
     misc_feature    3811..3828
     misc_feature    3811..3828
     misc_feature    3833..3865
     misc_binding    3888..4476
                     /label=WPRE
                     /note="""/ApEinfo_fwdcolor=#6948ff"
                     /note="/ApEinfo_revcolor=#6948ff"""
     polyA_signal    4519..4733
                     /label=bGHpolyA+
                     /label=bGHpA
                     /note="""/ApEinfo_fwdcolor=#ffdd18"
                     /note="/ApEinfo_revcolor=#ffdd18"""
     repeat_region   4819..4949
                     /label=3' ITR
                     /label=ITR 3'
                     /note="""/ApEinfo_fwdcolor=#c02a2f"
                     /note="/ApEinfo_revcolor=#c02a2f"""
     rep_origin      5455..6211
                     /label=pUC plasmid
     misc_feature    6275..6934
                     /label=/ampicilline=
                     /note="/ampicilline="
ORIGIN
        1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
       61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtggc caactccatc actaggggtt ccttgtagtt aatgattaac
      181 ccgccatgct acttatctac gtagccatgc tctaggtacc agatctgagc tcccacctcc
      241 ctctctgtgc tgggactcac agagggagac ctcaggaggc agtctgtcca tcacatgtcc
      301 aaatgcagag cataccctgg gctgggcgca gtggcgcaca actgtaattc cagcactttg
      361 ggaggctgat gtggaaggat cacttgagcc cagaagttct agaccagcct gggcaacatg
      421 gcaagaccct atctctacaa aaaaagttaa aaaatcagcc acgtgtggtg acacacacct
      481 gtagtcccag ctattcagga ggctgaggtg aggggatcac ttaaggctgg gaggttgagg
      541 ctgcagtgag tcgtggttgc gccactgcac tccagcctgg gcaacagtga gaccctgtct
      601 caaaagacaa aaaaaaaaaa aaaaaaaaaa agaacatatc ctggtgtgga gtaggggacg
      661 ctgctctgac agaggctcgg gggcctgagc tggctctgtg agctggggag gaggcagaca
      721 gccaggcctt gtctgcaagc agacctggca gcattgggct ggccgccccc cagggcctcc
      781 tcttcatgcc cagtgaatga ctcaccttgg cacagacaca atgttcgggg tgggcacagt
      841 gcctgcttcc cgccgcaccc cagcccccct caaatgcctt ccgagaagcc cattgagcag
      901 ggggcttgca ttgcacccca gcctgacagc ctggcatctt gggataaaag cagcacagcc
      961 ccctaggggc tgcccttgct gtgtggcgcc accggcggtg gagaacaagg ctctattcag
     1021 cctgtgccca ggaaagggga tcaggggatg cccaggcatg gacagtgggt ggcagggggg
     1081 gagaggaggg ctgtctgctt cccagaagtc caaggacaca aatgggtgag gggactgggc
     1141 agggttctga ccctgtggga ccagagtgga gggcgtagat ggacctgaag tctccaggga
     1201 caacagggcc caggtctcag gctcctagtt gggcccagtg gctccagcgt ttccaaaccc
     1261 atccatcccc agaggttctt cccatctctc caggctgatg tgtgggaact cgaggaaata
     1321 aatctccagt gggagacgga ggggtggcca gggaaacggg gcgctgcagg aataaagacg
     1381 agccagcaca gccagctcat gtgtaacggc tttgtggagc tgtcaaggcc tggtctctgg
     1441 gagagaggca cagggaggcc agacaaggaa ggggtgacct ggagggacag atccaggggc
     1501 taaagtcctg ataaggcaag agagtgccgg ccccctcttg ccctatcagg acctccactg
     1561 ccacatagag gccatgattg acccttagac aaagggctgg tgtccaatcc cagcccccag
     1621 ccccagaact ccagggaatg aatgggcaga gagcaggaat gtgggacatc tgtgttcaag
     1681 ggaaggactc caggagtctg ctgggaatga ggcctagtag gaaatgaggt ggcccttgag
     1741 ggtacagaac aggttcattc ttcgccaaat tcccagcacc ttgcaggcac ttacagctga
     1801 gtgagataat gcctgggtta tgaaatcaaa aagttggaaa gcaggtcaga ggtcatctgg
     1861 tacagccctt ccttcccttt tttttttttt ttttttgtga gacaaggtct ctctctgttg
     1921 cccaggctgg agtggcgcaa acacagctca ctgcagcctc aacctactgg gctcaagcaa
     1981 tcctccagcc tcagcctccc aaagtgctgg gattacaagc atgagccacc ccactcagcc
     2041 ctttccttcc tttttaattg atgcataata attgtaagta ttcatcatgg tccaaccaac
     2101 cctttcttga cccaccttcc tagagagagg gtcctcttgc ttcagcggtc agggccccag
     2161 acccatggtc tggctccagg taccacctgc ctcatgcagg agttggcgtg cccaggaagc
     2221 tctgcctctg ggcacagtga cctcagtggg gtgaggggag ctctccccat agctgggctg
     2281 cggcccaacc ccaccccctc aggctatgcc agggggtgtt gccaggggca cccgatcttc
     2341 ctcttcatgc ccagtgaatg actcaccttg gcacagacac aatgttcggg gtgggcacag
     2401 tgcctgcttc ccgccgcacc ccagcccccc tcaaatgcct tccgagaagc ccattgagca
     2461 ggcccgatct tcctcttcat gcccagtgaa tgactcacct tggcacagac acaatgttcg
     2521 gggtgggcac agtgcctgct tcccgccgca ccccagcccc cctcaaatgc cttccgagaa
     2581 gcccattgag caggcccgat cttcctcttc atgcccagtg aatgactcac cttggcacag
     2641 acacaatgtt cggggtgggc acagtgcctg cttcccgccg caccccagcc cccctcaaat
     2701 gccttccgag aagcccattg agcaggcccg ggcatcgcca gtctagccca ctccttcata
     2761 aagccctcgc atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc
     2821 tccgctgctc gcggggatcc tctagagtcg actagtggat cccccgggct gcaggaatta
     2881 tcacaagttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc gactggatcc
     2941 aggcctaagc ttacgcgtcc tagcgctacc ggtcgccacc atggtgagca agggcgagga
     3001 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3061 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3121 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     3181 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3241 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3301 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3361 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     3421 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     3481 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3541 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     3601 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3661 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgactagc
     3721 tagtagctag ctagctagtc gagctcaagc ttcgaattcg atatcaagct tatcgatacc
     3781 gtcgacctcg agatatctag acccagcttt cttgtacaaa gtggtgataa ttcgatatca
     3841 agcttatcga taccgtcgac ctcgatcgag atatcaagct tatcgataat caacctctgg
     3901 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3961 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4021 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4081 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4141 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4201 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4261 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     4321 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4381 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctg
     4441 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga ctcgctgatc
     4501 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4561 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4621 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4681 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4741 ggcggaaaga accagctggg gctcgactag agcatggcta cgtagataag tagcatggcg
     4801 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4861 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4921 cggcctcagt gagcgagcga gcgcgcagat aggcctccaa aaaagcctcc tcactacttc
     4981 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     5041 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     5101 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     5161 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
     5221 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgc
     5281 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5341 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5401 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5461 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5521 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5581 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5641 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5701 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5761 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5821 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5881 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5941 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6001 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6061 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6121 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6181 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6241 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6301 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6361 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6421 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6481 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6541 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6601 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6661 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6721 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6781 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6841 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6901 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6961 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7021 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7081 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7141 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7201 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7261 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     7321 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     7381 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     7441 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     7501 tactgagagt gcaccattcg acgctctccc ttatgcgact cctgcattag gaagcagccc
     7561 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg
     7621 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     7681 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     7741 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg
     7801 ctagcgatga ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca
     7861 gaaactcaga aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat
     7921 agggtctgct tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa
     7981 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
     8041 a
//
