LOCUS       Exported                6545 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Reu du groupe de M. During en 2001
            Sequence 1 ˆ 109 thŽorique prise sur vecteur 2343 
            pDEST-ssAAV-RFA-bGHpoly d'Alexi Demelmans 
            Enhancer immediate early gene CMV = identique vecteur AAV-CBA-hARSA 
            (Nathalie cartier) Chicken beta actin promoter (CBA) = identique 
            vecteur AAV-CBA-hARSA (Nathalie cartier) Intron chicken beta actin =
            identique vecteur AAV-CBA-hARSA (Nathalie cartier) jusqu'ˆ la 
            position 980
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     5'UTR           join(36..163,1201..1333)
                     /label=5' ITR
                     /label=ITR
                     /note="/SerialCloner_Color="
                     /note="""/SerialCloner_Show=True"
                     /note="/SerialCloner_Arrow=True"""
                     /note="/SerialCloner_Protect=False"
     promoter        join(227..769,1201..1304)
                     /label=GFA-ABC1D
                     /note="GFAP promoter of 681 bp from Dr M. Brenner 
                     corresponding to GFAP promoter sequences -1757 to -1256 / 
                     -132 to +47 [Split]"
     misc_feature    805..1197
                     /label=(B)3
                     /note="/(B)3="
     misc_feature    1356..1383
                     /label=phagemid pBluescriptII SK(+) [Split]
                     /note="phagemid pBluescriptII SK(+) [Split]"
     gene            join(1384..1394,2315..2332)
                     /label=/RFA= [Split]
                     /note="/RFA= [Split]"
     N_region        complement(1395..1411)
                     /label=attL1 [Split]
                     /note="attL1 [Split]"
     N_region        1472..2296
     CDS             1485..2196
                     /codon_start=1
                     /transl_table=11
                     /gene="egfp"
                     /label=egfp
                     /note="enhanced green fluorescent protein [Split]"
                     /db_xref="PID:g1377915"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDEL"
     N_region        2195..2202
     N_region        2195..2196
     N_region        join(2305..2332,3023..3259,3286..3453)
                     /label=attL2 [Split]
                     /note="attL2 [Split]"
     misc_feature    2315..2369
     misc_feature    2315..2369
     misc_feature    2315..2332
     misc_feature    2315..2332
     misc_feature    2315..2332
     misc_feature    2337..2369
     misc_binding    2392..2980
                     /label=WPRE
                     /note="""/ApEinfo_fwdcolor=#6948ff"
                     /note="/ApEinfo_revcolor=#6948ff"""
     polyA_signal    3023..3237
                     /label=bGHpolyA+
                     /label=bGHpA
                     /note="""/ApEinfo_fwdcolor=#ffdd18"
                     /note="/ApEinfo_revcolor=#ffdd18"""
     repeat_region   3323..3453
                     /label=3' ITR
                     /label=ITR 3'
                     /note="""/ApEinfo_fwdcolor=#c02a2f"
                     /note="/ApEinfo_revcolor=#c02a2f"""
     rep_origin      3959..4715
                     /label=pUC plasmid
     misc_feature    4779..5438
                     /label=/ampicilline=
                     /note="/ampicilline="
ORIGIN
        1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
       61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtggc caactccatc actaggggtt ccttgtagtt aatgattaac
      181 ccgccatgct acttatctac gtagccatgc tctaggtacc agatctaaca tatcctggtg
      241 tggagtaggg gacgctgctc tgacagaggc tcgggggcct gagctggctc tgtgagctgg
      301 ggaggaggca gacagccagg ccttgtctgc aagcagacct ggcagcattg ggctggccgc
      361 cccccagggc ctcctcttca tgcccagtga atgactcacc ttggcacaga cacaatgttc
      421 ggggtgggca cagtgcctgc ttcccgccgc accccagccc ccctcaaatg ccttccgaga
      481 agcccattga gcagggggct tgcattgcac cccagcctga cagcctggca tcttgggata
      541 aaagcagcac agccccctag gggctgccct tgctgtgtgg cgccaccggc ggtggagaac
      601 aaggctctat tcagcctgtg cccaggaaag gggatcaggg gatgcccagg catggacagt
      661 gggtggcagg gggggagagg agggctgtct gcttcccaga agtccaagga cacaaatggg
      721 tgaggggaga gctctcccca tagctgggct gcggcccaac cccaccccct caggctatgc
      781 cagggggtgt tgccaggggc acccgatctt cctcttcatg cccagtgaat gactcacctt
      841 ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt cccgccgcac cccagccccc
      901 ctcaaatgcc ttccgagaag cccattgagc aggcccgatc ttcctcttca tgcccagtga
      961 atgactcacc ttggcacaga cacaatgttc ggggtgggca cagtgcctgc ttcccgccgc
     1021 accccagccc ccctcaaatg ccttccgaga agcccattga gcaggcccga tcttcctctt
     1081 catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg cacagtgcct
     1141 gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt gagcaggccc
     1201 gggcatcgcc agtctagccc actccttcat aaagccctcg catcccagga gcgagcagag
     1261 ccagagcagg ttggagagga gacgcatcac ctccgctgct cgcggggatc ctctagagtc
     1321 gacggatccg gggaattcgc ggccgcactc gagatctagt ggatcccccg ggctgcagga
     1381 attatcacaa gtttgtacaa aaaagcaggc tccaccatgg gaaccaattc agtcgactgg
     1441 atccaggcct aagcttacgc gtcctagcgc taccggtcgc caccatggtg agcaagggcg
     1501 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1561 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1621 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1681 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1741 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1801 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1861 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1921 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1981 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2041 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2101 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     2161 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgac
     2221 tagctagtag ctagctagct agtcgagctc aagcttcgaa ttcgatatca agcttatcga
     2281 taccgtcgac ctcgagatat ctagacccag ctttcttgta caaagtggtg ataattcgat
     2341 atcaagctta tcgataccgt cgacctcgat cgagatatca agcttatcga taatcaacct
     2401 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2461 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2521 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2581 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2641 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2701 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2761 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     2821 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2881 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2941 cctgagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgactcgct
     3001 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3061 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3121 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3181 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3241 ctgaggcgga aagaaccagc tggggctcga ctagagcatg gctacgtaga taagtagcat
     3301 ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg
     3361 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     3421 cgggcggcct cagtgagcga gcgagcgcgc agataggcct ccaaaaaagc ctcctcacta
     3481 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     3541 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     3601 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     3661 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     3721 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     3781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3961 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4021 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4081 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4141 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4201 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4261 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4321 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4381 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4441 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4621 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4681 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4741 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4801 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4861 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4921 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4981 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5041 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5101 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5161 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5221 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5281 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5341 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5401 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5461 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5521 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5581 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5641 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5701 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5761 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5821 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5881 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5941 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     6001 attgtactga gagtgcacca ttcgacgctc tcccttatgc gactcctgca ttaggaagca
     6061 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
     6121 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     6181 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     6241 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
     6301 ctggctagcg atgaccctgc tgattggttc gctgaccatt tccgggtgcg ggacggcgtt
     6361 accagaaact cagaaggttc gtccaaccaa accgactctg acggcagttt acgagagaga
     6421 tgatagggtc tgcttcagta agccagatgc tacacaatta ggcttgtaca tattgtcgtt
     6481 agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca
     6541 ctata
//
