LOCUS       Exported                6746 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Reu du groupe de M. During en 2001
            Sequence 1 ˆ 109 thŽorique prise sur vecteur 2343 
            pDEST-ssAAV-RFA-bGHpoly d'Alexi Demelmans 
            Enhancer immediate early gene CMV = identique vecteur AAV-CBA-hARSA 
            (Nathalie cartier) Chicken beta actin promoter (CBA) = identique 
            vecteur AAV-CBA-hARSA (Nathalie cartier) Intron chicken beta actin =
            identique vecteur AAV-CBA-hARSA (Nathalie cartier) jusqu'ˆ la 
            position 980 Sequenage 30.9.13 montre 4 nucleotides de plus TGGA en
            pos 1154 et modifie G en C pos 1155 (avant insertion TGGA).
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1250..1273
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     source          1274..1658
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     5'UTR           36..163
                     /label=5' ITR
                     /label=ITR
                     /note="/SerialCloner_Color="
                     /note="""/SerialCloner_Show=True"
                     /note="/SerialCloner_Arrow=True"""
                     /note="/SerialCloner_Protect=False"
     promoter        219..464
                     /label=enhancer immediate early gene CMV
                     /note="CMV immediate early region"
     promoter        466..747
                     /label=chicken beta actin promoter (CBA)
                     /note="/CBA promoter="
     intron          747..1057
                     /label=CBA intron
                     /note="/CBA intron="
     intron          1058..1158
                     /label=Rabbit beta globin Intron
                     /note="/Intron rabbit beta glogin="
     misc_feature    join(1164..1197,2574..2577)
                     /label=phagemid pBluescriptII SK(+) [Split]
                     /note="phagemid pBluescriptII SK(+) [Split]"
     gene            1198..1208
                     /label=/RFA= [Split]
                     /note="/RFA= [Split]"
     misc_feature    join(1209..1225,1250..1273)
                     /label=attL1 [Split]
                     /note="attL1 [Split]"
     misc_feature    1247..1249
                     /label= [Split]
                     /note=" [Split]"
     CDS             1281..1658
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             1671..2381
                     /codon_start=1
                     /label=Cherry
                     /label=mCherry
                     /note="vntifkey=4"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             2396..2450
                     /codon_start=1
                     /translation="EMGEAN*ASLVMHRAAGI"
     CDS             complement(2396..2449)
                     /codon_start=1
                     /translation="NSCSPVHD*ASSVSLPHL"
     CDS             2484..2501
                     /codon_start=1
                     /translation="LVQSGD"
     CDS             2502..2528
                     /codon_start=1
                     /translation="NSISSLSIP"
     CDS             2502..2528
                     /codon_start=1
                     /translation="NSISSLSIP"
     CDS             complement(2502..2528)
                     /codon_start=1
                     /translation="RYR*A*YRI"
     misc_feature    2506..2528
                     /label=phagemid pBluescriptII SK(+) [Split]
                     /note="phagemid pBluescriptII SK(+) [Split]"
     misc_feature    join(2542..2551,3224..3460,3487..3654)
                     /label=attL2 [Split]
                     /note="attL2 [Split]"
     intron          2552..2577
     intron          2552..2577
     gene            complement(2552..2569)
     gene            2552..2569
     gene            2552..2569
     gene            2552..2569
     gene            2552..2569
     misc_binding    2593..3181
                     /label=WPRE
                     /note="""/ApEinfo_fwdcolor=#6948ff"
                     /note="/ApEinfo_revcolor=#6948ff"""
     polyA_signal    3224..3438
                     /label=bGHpolyA+
                     /label=bGHpA
                     /note="""/ApEinfo_fwdcolor=#ffdd18"
                     /note="/ApEinfo_revcolor=#ffdd18"""
     repeat_region   3524..3654
                     /label=3' ITR
                     /label=ITR 3'
                     /note="""/ApEinfo_fwdcolor=#c02a2f"
                     /note="/ApEinfo_revcolor=#c02a2f"""
     rep_origin      4160..4916
                     /label=pUC plasmid
     misc_feature    4980..5639
                     /label=/ampicilline=
                     /note="/ampicilline="
ORIGIN
        1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
       61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtggc caactccatc actaggggtt ccttgtagtt aatgattaac
      181 ccgccatgct acttatctac gtagccatgc tctaggtacc attgacgtca ataatgacgt
      241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
      481 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
      541 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag
      601 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
      661 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
      721 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
      781 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
      841 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
      901 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
      961 gggggagcgg ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     1021 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     1081 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     1141 tttggcaaag aattggatct tccctagaac tagtggatcc cccgggctgc aggaattatc
     1201 acaagtttgt acaaaaaagc aggctccacc atgggaacca attcagtcgc gtttagtgaa
     1261 ccgtcagagg taccgccacc atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg
     1321 gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc
     1381 gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac cctgacaccg
     1441 gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt ttcgagcgca
     1501 tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc acctccaggg
     1561 agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg
     1621 agggtactaa ggccatcacc aagtacacca gcgctaagga tcccgccacc atggtgagca
     1681 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     1741 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1801 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     1861 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     1921 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1981 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     2041 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     2101 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     2161 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     2221 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     2281 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     2341 gccgccactc caccggcggc atggacgagc tgtacaagta aagcggccgc gtcgagagat
     2401 gggggaggct aactgagcta gcttagtcat gcaccgggct gcaggaattc gcggccgcac
     2461 tcgagatatc tagacccagc tttcttgtac aaagtggtga taattcgata tcaagcttat
     2521 cgataccgtc gagatatcta gacccagctt tcttgtacaa agtggtgata attcgatatc
     2581 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2641 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2701 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2761 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2821 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2881 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2941 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     3001 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     3061 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3121 cttccgcgtc ttcgccttcg ccctgagacg agtcggatct ccctttgggc cgcctccccg
     3181 catcgatacc gtcgactcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     3241 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3301 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     3361 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     3421 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg actagagcat
     3481 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg
     3541 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3601 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagataggcc
     3661 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     3721 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     3781 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     3841 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     3901 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     3961 taactgacac acattccaca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4021 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4081 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4381 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     4441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4621 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     4681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4981 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5041 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5101 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5161 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5221 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5281 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5341 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5401 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5461 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5521 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5581 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5641 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5701 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5761 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5821 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5881 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5941 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     6001 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     6061 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     6121 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     6181 aactatgcgg catcagagca gattgtactg agagtgcacc attcgacgct ctcccttatg
     6241 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     6301 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     6361 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     6421 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     6481 gatgcgtccg gcgtagagga tctggctagc gatgaccctg ctgattggtt cgctgaccat
     6541 ttccgggtgc gggacggcgt taccagaaac tcagaaggtt cgtccaacca aaccgactct
     6601 gacggcagtt tacgagagag atgatagggt ctgcttcagt aagccagatg ctacacaatt
     6661 aggcttgtac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
     6721 acacatacga tttaggtgac actata
//
