LOCUS       Exported                9910 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     BamHI site at position 2628
            XhoI site at position 3331
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          9608..9729
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     repeat_region   1..634
                     /label=5'LTR
                     /note="5'LTR"
     5'UTR           1..634
                     /label=5'LTR
                     /note="5'LTR"
     misc_feature    1..455
                     /label=U3
                     /note="U3"
     misc_feature    454..551
                     /label=R
                     /note="R"
     misc_feature    456..552
                     /label=R
                     /note="R"
     misc_feature    553..634
                     /label=U5
                     /note="U5"
     misc_feature    687..823
                     /label=psi
                     /note="psi"
     gene            1152..2009
                     /label=RRE (long)
                     /note="/RRE (long)="
     gene            2016..2034
                     /label=shUNIV
                     /note="shUNIV"
     promoter        2349..2707
                     /label=mDR1
                     /note="mD1R"
     misc_feature    2751..2780
                     /label=phagemid pBluescriptII SK(+) [Split]
                     /note="phagemid pBluescriptII SK(+) [Split]"
     old_sequence    2784..2808
                     /label=attB1
                     /note="attB1"
     misc_feature    2866..2876
                     /label=Kozak consensus sequence
                     /note="Kozak consensus sequence"
     gene            2996..3014
                     /label=shGFP
                     /note="shGFP"
                     /note="/bound_moiety="
     gene            complement(2998..3014)
                     /label=shGFP
                     /note="shGFP"
     sig_peptide     3611..3686
                     /label=nls
     old_sequence    3716..3740
                     /label=attB2
                     /note="attB2"
     CDS             4203..4381
                     /codon_start=1
                     /label=X protein
                     /note="X protein"
                     /db_xref="PID:g336148"
                     /translation="MAARLCCHLDSARDVLLLRPFGPQSSGPSFPRPAAGSAASSASSP
                     SPSDESDLPLGRLP"
     3'UTR           4470..4703
                     /label=3'LTR
                     /note="3'LTR"
     misc_feature    4470..4524
                     /label=deltaU3
                     /note="delU3"
     repeat_region   4508..4711
                     /label=3'LTR
                     /note="[Segment 2 of 2]"
     misc_feature    4523..4620
                     /label=R
                     /note="R"
     misc_feature    4525..4621
                     /label=R
                     /note="R"
     misc_feature    4621..4711
                     /label=U5
                     /note="U5"
     promoter        complement(4730..4747)
                     /note="SP6 promoter"
     gene            5640..6500
                     /label=AmpR
                     /note="AmpR"
     rep_origin      6501..7434
                     /label=ColE1
                     /note="ColE1"
     rep_origin      7734..7809
                     /direction=BOTH
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    9608..9729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9645..9664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9699..9718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt ggacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgataa gcttgatatc gaattatcac aagtttgtac aaaaaagcag gctccgcggc
     2101 cgcccccttc accatcgata acgctgcctg tcaagcgagg accgccccca gggcagggaa
     2161 gcttctggaa cccagccggc cggtgccctc gctcgctcga gctgttgtgc ttgcctggag
     2221 ctcctgccac tgctgacagg gagcgagcgg gtggctcccc aaggaggctc agggtcctgc
     2281 cctaagaacg aggagccaaa ggggtgccca gagctgggct tcctcagaca ggacagaagc
     2341 tgttctgcag taggtggtct tgatggactt gtccaggggg tgatgggctg agattttgtg
     2401 tagccaaata ggaggtctgc acagagtatt ggtgaacgac tttgtgtttc cctttgcttt
     2461 ttagggcatt tggagagatg tggcaccagg ggcttgaagg agaaatgcat gtattttagg
     2521 ctgtgtctca gaagaagagg cagcatccct gaaaagtgac tgagattgac caggaagagg
     2581 ccccagactc ttagagtatg cttaaaagcc tatgcattcc ttggggaatc tagggaccag
     2641 cggatgccag ggaacagcga cctggagcac tgaacccaga agacaggtgg aaagcaagcg
     2701 gctcttctgg atcaaactta agcttggtac cgagctcgga tcttccctag aactagtgga
     2761 tcccccgggc tgcaggaatt atcacaagtt tgtacaaaaa agcaggctcc accatgggaa
     2821 ccaattcagt cgactggatc cggtaccgaa tctagcgcta ccggtcgcca ccatggtgag
     2881 caagggcgcc gagctgttca ccggcatcgt gcccatcctg atcgagctga atggcgatgt
     2941 gaatggccac aagttcagcg tgagcggcga gggcgagggc gatgccacct acggcaagct
     3001 gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg ccctggccca ccctggtgac
     3061 caccctgagc tacggcgtgc agtgcttctc acgctacccc gatcacatga agcagcacga
     3121 cttcttcaag agcgccatgc ctgagggcta catccaggag cgcaccatct tcttcgagga
     3181 tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag ggcgataccc tggtgaatcg
     3241 catcgagctg accggcaccg atttcaagga ggatggcaac atcctgggca ataagatgga
     3301 gtacaactac aacgcccaca atgtgtacat catgaccgac aaggccaaga atggcatcaa
     3361 ggtgaacttc aagatccgcc acaacatcga ggatggcagc gtgcagctgg ccgaccacta
     3421 ccagcagaat acccccatcg gcgatggccc tgtgctgctg cccgataacc actacctgtc
     3481 cacccagagc gccctgtcca aggaccccaa cgagaagcgc gatcacatga tctacttcgg
     3541 cttcgtgacc gccgccgcca tcacccacgg catggatgag ctgtacaagt ccggactcag
     3601 atctcgagct gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtagg
     3661 tccaaaaaag aagagaaagg taggatcaat tcgcggccgc actcgagata tctagaccca
     3721 gctttcttgt acaaagtggt gataattcga tatcaagctt atcgataccg tcgacctcga
     3781 gggaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3961 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4021 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4081 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4141 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     4201 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4261 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4321 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4381 catcgggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     4441 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     4501 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4561 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4621 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     4681 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     4741 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     4801 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     4861 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     4921 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     4981 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     5041 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     5101 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     5161 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     5221 ctaatgcagg agtcgcataa gggagagcgt cgatatggtg cactctcagt acaatctgct
     5281 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5341 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5401 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5461 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     5521 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5581 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5641 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5701 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5761 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5821 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5881 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5941 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6001 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6061 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6121 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6181 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6241 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6301 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6361 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6421 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6481 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6541 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6601 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6661 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6721 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6781 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6841 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6901 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6961 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7021 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7081 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7141 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7201 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7261 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7321 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7381 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7441 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     7501 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7561 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7681 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7741 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7801 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     7861 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     7921 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     7981 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     8041 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     8101 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     8161 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     8221 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     8281 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     8341 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     8401 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     8461 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     8521 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     8581 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     8641 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     8701 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     8761 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     8821 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     8881 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     8941 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     9001 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     9061 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     9121 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     9181 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     9241 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     9301 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     9361 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     9421 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     9481 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag agcttttact
     9541 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     9601 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9661 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9721 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9781 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct
     9841 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     9901 acttagtagt
//
