LOCUS       Exported               11195 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     3'-LTR OK
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          10893..11014
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     repeat_region   1..634
                     /label=5'LTR
                     /note="5'LTR"
     5'UTR           1..634
                     /label=5'LTR
                     /note="5'UTR"
     misc_feature    1..455
                     /label=U3
                     /note="U3"
     misc_feature    454..551
                     /label=R
                     /note="R"
     misc_feature    456..552
                     /label=R
                     /note="R"
     misc_feature    553..634
                     /label=U5
                     /note="U5"
     misc_feature    687..823
                     /label=psi
                     /note="psi"
     gene            1152..2009
                     /label=RRE (long)
                     /note="/RRE (long)="
     gene            2016..2034
                     /label=shUNIV
                     /note="shUNIV"
     promoter        2126..3813
                     /label=rNSE promoter
                     /note="/note = 652 to 2562 of pENTR/D-TOPO-NSE promoteur"
     misc_feature    4002..4031
                     /label=phagemid pBluescriptII SK(+) [Split]
                     /note="phagemid pBluescriptII SK(+) [Split]"
     misc_feature    4151..4160
                     /label=Kozak
                     /note="Kozak consensus translation initiation site"
     gene            4158..5000
                     /label=AcGFP1nuc
                     /note="AcGFP1nuc"
     gene            complement(4283..4299)
                     /label=shGFP
                     /note="shGFP"
     sig_peptide     4893..4967
                     /label=nls
                     /note="nuclear localization signal"
     CDS             5488..5666
                     /codon_start=1
                     /label=X protein
                     /note="X protein"
                     /db_xref="PID:g336148"
                     /translation="MAARLCCHLDSARDVLLLRPFGPQSSGPSFPRPAAGSAASSASSP
                     SPSDESDLPLGRLP"
     3'UTR           5755..5988
                     /label=3'LTR
                     /note="3'LTR"
     misc_feature    5755..5809
                     /label=deltaU3
                     /note="delU3"
     repeat_region   5793..5996
                     /label=3'LTR
                     /note="[Segment 2 of 2]"
     misc_feature    5808..5905
                     /label=R
                     /note="R"
     misc_feature    5810..5906
                     /label=R
                     /note="R"
     misc_feature    5906..5996
                     /label=U5
                     /note="U5"
     promoter        complement(6015..6032)
                     /note="SP6 promoter"
     gene            6925..7785
                     /label=AmpR
                     /note="AmpR"
     rep_origin      7786..8719
                     /label=ColE1
                     /note="ColE1"
     rep_origin      9019..9094
                     /direction=BOTH
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    10893..11014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10930..10949)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10984..11003
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgataa gcttgatatc gaattatcac aaggtttgta caaaaaagca ggctccgcgg
     2101 ccgccccctt caccatcgat gtaccgagct cctcctctgc tcgcccaatc cttccaaccc
     2161 cctatggtgg tatggctgac acagaaaatg tctgctcctg tatgggacat ttgcccctct
     2221 tctccaaata taagacagga tgaggcctag cttttgctgc tccaaagttt taaaagaaca
     2281 cattgcacgg catttaggga ctctaaaggg tggaggagga atgagggaat tgcatcatgc
     2341 caaggctggt cctcatccat cactgcttcc agggcccaga gtggcttcca ggaggtattc
     2401 ttacaaagga agcccgatct gtagctaaca ctcagagccc attttcctgc gttaacccct
     2461 cccgacctca tatacaggag taacatgatc agtgacctgg gggagctggc caaactgcgg
     2521 gacctgccca agctgagggc cttggtgctg ctggacaacc cctgtgccga tgagactgac
     2581 taccgccagg aggccctggt gcagatggca cacctagagc gcctagacaa agagtactat
     2641 gaggacgagg accgggcaga agctgaggag atccgacaga ggctgaagga ggaacaggag
     2701 caagaactcg acccggacca agacatggaa ccgtacctcc cgccaactta gtggctcctc
     2761 tagcctgcag ggacagtaaa ggtgatggca ggaaggcagc ccccggaggt caaaggctgg
     2821 gcacgcggga ggagaggcca gagtcagagg ctgcgggtat ctcagatatg aaggaaagat
     2881 gagagaggct caggaagagg taagaaaaga cacaagagac cagagaaggg agaagaatta
     2941 gagagggagg cagaggaccg ctgtctctac agacatagct ggtagagact gggaggaagg
     3001 gatgaaccct gagcgcatga agggaaggag gtggctggtg gtatatggag gatgtagctg
     3061 gggccaggga aaagatcctg cactggggat ctgaagctgg ggagaacagg acacggggtg
     3121 gagaggcgaa aggagggcag agtgaagcag agagactgag gcctggggat gtgggcattc
     3181 cggtagggca cacagttcac ttgtcttctc tttttccagg aggccaaaga tgctgacgtc
     3241 aagaactcat aataccccag tggggaccac cgcattcata gccctgttac aagaagtggg
     3301 agatgttcct ttttgtccca gactggaaat ccattacatc ccgaggctca ggttctgtgg
     3361 tggtcatctc tgtgtggctt gttctgtggg cctacctaaa gtcctaagca cagctctcaa
     3421 gcagatccga ggcgactaag atgctagtag gggttgtctg gagagaagag ccgaggaggt
     3481 gggctgtgat ggatcagttc agctttcaaa taaaaaggcg tttttatatt ctgtgtcgag
     3541 ttcgtgaacc cctgtggtgg gcttctccat ctgtctgggt tagtacctgc cactatactg
     3601 gaataagggg acgcctgctt ccctcgagtt ggctggacaa ggttatgagc atccgtgtac
     3661 ttatggggtt gccagcttgg tcctggatcg cccgggccct tcccccaccc gttcggttcc
     3721 ccaccaccac ccgcgctcgt acgtgcgtct ccgcctgcag ctcttgactc atcggggccc
     3781 ccgggtcaca tgcgctcgct cggctctata ggcgccgccc cctgcccacc ccccgcccgc
     3841 gctgggagcc gcagccgccg ccactcctgc tctctctgcg ccgccgccgt caccaccgcc
     3901 accgccaccg gctgagtctg cagtcctcga gatccaccgg tcgccaccat ggtccgcaag
     3961 ggtgggcgcg ccgacccagc tttcttgtac aaagtggtga taattcctgc agcccggggg
     4021 atccactagt tctaggatcc accatgtcta ggggctgcag gaattatcac aagtttgtac
     4081 aaaaaagcag gctccaccat gggaaccaat tcagtcgact ggatccggta ccgaatctag
     4141 cgctaccggt cgccaccatg gtgagcaagg gcgccgagct gttcaccggc atcgtgccca
     4201 tcctgatcga gctgaatggc gatgtgaatg gccacaagtt cagcgtgagc ggcgagggcg
     4261 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4321 ctgtgccctg gcccaccctg gtgaccaccc tgagctacgg cgtgcagtgc ttctcacgct
     4381 accccgatca catgaagcag cacgacttct tcaagagcgc catgcctgag ggctacatcc
     4441 aggagcgcac catcttcttc gaggatgacg gcaactacaa gtcgcgcgcc gaggtgaagt
     4501 tcgagggcga taccctggtg aatcgcatcg agctgaccgg caccgatttc aaggaggatg
     4561 gcaacatcct gggcaataag atggagtaca actacaacgc ccacaatgtg tacatcatga
     4621 ccgacaaggc caagaatggc atcaaggtga acttcaagat ccgccacaac atcgaggatg
     4681 gcagcgtgca gctggccgac cactaccagc agaatacccc catcggcgat ggccctgtgc
     4741 tgctgcccga taaccactac ctgtccaccc agagcgccct gtccaaggac cccaacgaga
     4801 agcgcgatca catgatctac ttcggcttcg tgaccgccgc cgccatcacc cacggcatgg
     4861 atgagctgta caagtccgga ctcagatctc gagctgatcc aaaaaagaag agaaaggtag
     4921 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagga tcaattcgcg
     4981 gccgcactcg agatatctag acccagcttt cttgtacaaa gtggtgataa ttcgatatca
     5041 agcttatcga taccgtcgac ctcgagggaa ttccgataat caacctctgg attacaaaat
     5101 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5161 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5221 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5281 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5341 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5401 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5461 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     5521 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5581 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5641 gatctccctt tgggccgcct ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa
     5701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5761 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5821 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5881 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5941 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     6001 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     6061 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     6121 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
     6181 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
     6241 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     6301 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     6361 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     6421 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     6481 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgata
     6541 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     6601 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6661 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6721 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6781 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6841 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6901 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6961 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7021 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     7081 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     7141 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     7201 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     7261 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     7321 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7381 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7441 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7501 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7561 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7621 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7681 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7741 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7801 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7861 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7921 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7981 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8041 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     8101 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     8161 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     8221 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     8281 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     8341 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8401 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8461 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8521 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8581 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8641 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8701 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8761 tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     8821 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     8881 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8941 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     9001 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     9061 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     9121 agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca
     9181 gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca
     9241 gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa
     9301 caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc
     9361 catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc
     9421 gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg
     9481 tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga
     9541 aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg
     9601 gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa
     9661 aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga
     9721 ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt
     9781 ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt
     9841 ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc
     9901 aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca
     9961 caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt
    10021 aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca
    10081 cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc
    10141 taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt
    10201 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
    10261 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
    10321 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
    10381 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
    10441 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
    10501 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
    10561 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
    10621 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
    10681 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
    10741 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
    10801 ataccacatt tgtagagctt ttacttgctt taaaaaacct cccacacctc cccctgaacc
    10861 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
    10921 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10981 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
    11041 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
    11101 tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    11161 tgtatttgtt aaatatgtac tacaaactta gtagt
//
