LOCUS       Exported               10185 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     3'-LTR OK
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          9883..10004
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     repeat_region   1..634
                     /label=5'LTR
                     /note="5'LTR"
     3'UTR           1..634
                     /label=5'LTR
                     /note="5'LTR"
     misc_feature    1..455
                     /label=U3
                     /note="U3"
     misc_feature    454..551
                     /label=R
                     /note="R"
     misc_feature    456..552
                     /label=R
                     /note="R"
     misc_feature    553..634
                     /label=U5
                     /note="U5"
     misc_feature    687..823
                     /label=psi
                     /note="psi"
     gene            1152..2009
                     /label=RRE (long)
                     /note="/RRE (long)="
     gene            2016..2034
                     /label=shUNIV
                     /note="shUNIV"
     promoter        2116..2983
                     /label=TAC promoter
                     /note="Promoter Rat TAC"
     misc_feature    3141..3150
                     /label=Kozak
                     /note="Kozak consensus translation initiation site"
     gene            3148..3990
                     /label=AcGFPnuc
                     /note="AcGFP1-nuc"
     gene            complement(3273..3289)
                     /label=shGFP
                     /note="shGFP"
     sig_peptide     3883..3957
                     /label=nls
                     /note="Nuclear localization signal"
     CDS             4478..4656
                     /codon_start=1
                     /label=X protein
                     /note="X protein"
                     /db_xref="PID:g336148"
                     /translation="MAARLCCHLDSARDVLLLRPFGPQSSGPSFPRPAAGSAASSASSP
                     SPSDESDLPLGRLP"
     3'UTR           4745..4978
                     /label=3'LTR
                     /note="3'LTR"
     misc_feature    4745..4799
                     /label=deltaU3
                     /note="delU3"
     repeat_region   4783..4986
                     /label=3'LTR
                     /note="[Segment 2 of 2]"
     misc_feature    4798..4895
                     /label=R
                     /note="R"
     misc_feature    4800..4896
                     /label=R
                     /note="R"
     misc_feature    4896..4986
                     /label=U5
                     /note="U5"
     promoter        complement(5005..5022)
                     /note="SP6 promoter"
     gene            5906..6706
                     /label=Ampicilline gene resistance
                     /note="Ampicilline gene resistance"
     gene            5915..6775
                     /label=AmpR
                     /note="AmpR"
     rep_origin      6776..7709
                     /label=ColE1
                     /note="ColE1"
     rep_origin      8009..8084
                     /direction=BOTH
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    9883..10004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9920..9939)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9974..9993
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgataa gcttgatatc gaattatcac aagtttgtac aaaaaagcag gctccgcggc
     2101 cgcccccttc accacctgca gagctccaaa ggtaagcatc cagcctttct agtcccccaa
     2161 caaggctaaa ggggagagag gcacaattat cctcttccca ccccttctgc cttcagggtg
     2221 tgcctgggaa gaagctgtag gggaacaaaa gatgccttag aatggctgat gggtaagttc
     2281 tacatgagaa aggaggttta aattcctctt tcccctaaat gtaaaacaaa cctgccttca
     2341 tcctctgaag cgggagaccg gaaacacttt tgcagtgcta gagaaatgag aatattctga
     2401 ctgatttggt ggggaggggg gttggggggg tgtgttccag ccctagatat aacacctcat
     2461 aaaccttaag acacataaag tagaaatgaa aggaaaaccc cgcttgcttc atccctctga
     2521 agtgcttgct ggtgtcttag tattattcac aaggttttgc tgctcaagtt atttggctgt
     2581 cctcaaagcg caatattccc tgatgcctct tgagagaaaa gttccctaag tccgaagcat
     2641 gagtcacttc gctcagtttt gatgagtaat ctcaggtgtc actgaacctt gttcggaaga
     2701 agaggggagg ggggcgtcag atttgcagac ggaagaaaac aggtctctct ggattggatg
     2761 gcgagacctc gacttcccta aaattgcgtc atttcgaacc caatttggtc cagatgttat
     2821 ggactccgac gggttaccgt ctcggaaact ctatcacgca agcaaaaggc gagggggcgg
     2881 ctaattaaat attgagcaga aagtcgcgtg gggagagtgt cacgtggctc tccaggctca
     2941 tcacgcctga gataaataag gcgaagcagg agcagggact agaaagggtg ggcgcgccga
     3001 cccagctttc ttgtacaaag tggtgataat tcctgcagcc cgggctgcag gaattatcac
     3061 aagtttgtac aaaaaagcag gctccaccat gggaaccaat tcagtcgact ggatccggta
     3121 ccgaatctag cgctaccggt cgccaccatg gtgagcaagg gcgccgagct gttcaccggc
     3181 atcgtgccca tcctgatcga gctgaatggc gatgtgaatg gccacaagtt cagcgtgagc
     3241 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3301 ggcaagctgc ctgtgccctg gcccaccctg gtgaccaccc tgagctacgg cgtgcagtgc
     3361 ttctcacgct accccgatca catgaagcag cacgacttct tcaagagcgc catgcctgag
     3421 ggctacatcc aggagcgcac catcttcttc gaggatgacg gcaactacaa gtcgcgcgcc
     3481 gaggtgaagt tcgagggcga taccctggtg aatcgcatcg agctgaccgg caccgatttc
     3541 aaggaggatg gcaacatcct gggcaataag atggagtaca actacaacgc ccacaatgtg
     3601 tacatcatga ccgacaaggc caagaatggc atcaaggtga acttcaagat ccgccacaac
     3661 atcgaggatg gcagcgtgca gctggccgac cactaccagc agaatacccc catcggcgat
     3721 ggccctgtgc tgctgcccga taaccactac ctgtccaccc agagcgccct gtccaaggac
     3781 cccaacgaga agcgcgatca catgatctac ttcggcttcg tgaccgccgc cgccatcacc
     3841 cacggcatgg atgagctgta caagtccgga ctcagatctc gagctgatcc aaaaaagaag
     3901 agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagga
     3961 tcaattcgcg gccgcactcg agatatctag acccagcttt cttgtacaaa gtggtgataa
     4021 ttcgatatca agcttatcga taccgtcgac ctcgagggaa ttccgataat caacctctgg
     4081 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4141 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4201 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4261 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4321 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4381 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4441 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     4501 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4561 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4621 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcgag ctcggtacct
     4681 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     4741 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     4801 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4861 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4921 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     4981 atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
     5041 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
     5101 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
     5161 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
     5221 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     5281 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     5341 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     5401 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     5461 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     5521 agcgtcgata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5581 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5641 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5701 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5761 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5881 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5941 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6001 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6061 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6121 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6181 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6241 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6301 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6361 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6421 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6481 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6541 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6601 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6661 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6721 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6781 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6841 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6901 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6961 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7021 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7081 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7141 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7201 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7261 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7321 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7381 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7441 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7501 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7561 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7621 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7681 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7741 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     7801 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7861 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7921 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7981 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8041 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8101 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     8161 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     8221 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     8281 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     8341 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     8401 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     8461 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     8521 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     8581 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     8641 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     8701 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     8761 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     8821 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     8881 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     8941 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
     9001 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
     9061 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
     9121 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
     9181 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     9241 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     9301 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     9361 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     9421 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     9481 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     9541 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     9601 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     9661 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     9721 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     9781 ataatcagcc ataccacatt tgtagagctt ttacttgctt taaaaaacct cccacacctc
     9841 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     9901 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9961 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    10021 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    10081 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    10141 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
