LOCUS       Exported                9706 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     3'-LTR OK
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          9404..9525
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     5'UTR           1..634
                     /label=5'LTR
                     /note="5'LTR"
     repeat_region   1..634
                     /label=5'LTR
                     /note="5'LTR"
     misc_feature    1..455
                     /label=U3
                     /note="U3"
     misc_feature    454..551
                     /label=R
                     /note="R"
     misc_feature    456..552
                     /label=R
                     /note="R"
     misc_feature    553..634
                     /label=U5
                     /note="U5"
     misc_feature    687..823
                     /label=psi
                     /note="psi"
     gene            1152..2009
                     /label=RRE (long)
                     /note="/RRE (long)="
     gene            2016..2034
                     /label=shUNIV
                     /note="shUNIV"
     promoter        2086..2594
                     /label=mPGK
                     /note="mPGK1 promoter (-518/-15)"
     primer_bind     2515..2538
                     /label=ER-8
                     /note="ER-8"
     primer_bind     2527..2544
                     /label=PGK-5R (#31)
                     /note="PGK-5R (#31)"
     misc_feature    2691..2700
                     /label=Kozak
                     /note="Kozak consensus translation initiation site"
     gene            2698..3540
                     /label=AcGFP
                     /note="AcGFP1-nuc"
     gene            complement(2823..2839)
                     /label=shGFP
                     /note="shGFP"
     sig_peptide     3433..3507
                     /label=nls
                     /note="nuclear localization signal"
     enhancer        3566..4153
                     /note="WPRE"
     CDS             3995..4173
                     /codon_start=1
                     /label=X protein
                     /note="X protein"
                     /db_xref="PID:g336148"
                     /translation="MAARLCCHLDSARDVLLLRPFGPQSSGPSFPRPAAGSAASSASSP
                     SPSDESDLPLGRLP"
     3'UTR           4262..4495
                     /label=3'LTR
                     /note="3'LTR"
     misc_feature    4262..4316
                     /label=deltaU3
                     /note="delU3"
     repeat_region   4300..4503
                     /label=3'LTR
                     /note="[Segment 2 of 2]"
     misc_feature    4315..4412
                     /label=R
                     /note="R"
     misc_feature    4317..4413
                     /label=R
                     /note="R"
     misc_feature    4413..4503
                     /label=U5
                     /note="U5"
     promoter        complement(4526..4543)
                     /note="SP6 promoter"
     gene            5436..6296
                     /label=AmpR
                     /note="AmpR"
     rep_origin      6297..7230
                     /label=ColE1
                     /note="ColE1"
     rep_origin      7530..7605
                     /direction=BOTH
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    9404..9525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9441..9460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9495..9514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgatgg ccatcgatgg ccatcgatgg ccatcgatgg tcgagtaccg ggtaggggag
     2101 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     2161 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     2221 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     2281 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     2341 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     2401 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     2461 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     2521 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     2581 ggcctttcga cctctagatc ggatcatcac aagtttgtac aaaaaagcag gctccaccat
     2641 gggaaccaat tcagtcgact ggatccggta ccgaatctag cgctaccggt cgccaccatg
     2701 gtgagcaagg gcgccgagct gttcaccggc atcgtgccca tcctgatcga gctgaatggc
     2761 gatgtgaatg gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc cacctacggc
     2821 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg
     2881 gtgaccaccc tgagctacgg cgtgcagtgc ttctcacgct accccgatca catgaagcag
     2941 cacgacttct tcaagagcgc catgcctgag ggctacatcc aggagcgcac catcttcttc
     3001 gaggatgacg gcaactacaa gtcgcgcgcc gaggtgaagt tcgagggcga taccctggtg
     3061 aatcgcatcg agctgaccgg caccgatttc aaggaggatg gcaacatcct gggcaataag
     3121 atggagtaca actacaacgc ccacaatgtg tacatcatga ccgacaaggc caagaatggc
     3181 atcaaggtga acttcaagat ccgccacaac atcgaggatg gcagcgtgca gctggccgac
     3241 cactaccagc agaatacccc catcggcgat ggccctgtgc tgctgcccga taaccactac
     3301 ctgtccaccc agagcgccct gtccaaggac cccaacgaga agcgcgatca catgatctac
     3361 ttcggcttcg tgaccgccgc cgccatcacc cacggcatgg atgagctgta caagtccgga
     3421 ctcagatctc gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
     3481 gtagatccaa aaaagaagag aaaggtagga tcaattcgcg gccgcactcg agatatctag
     3541 acccagcttt cttgtacaaa gtggtgattc gagggaattc cgataatcaa cctctggatt
     3601 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3661 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3721 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3781 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3841 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3901 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3961 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     4021 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4081 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4141 cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcgagctc ggtaccttta
     4201 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4261 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     4321 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4381 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4441 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc
     4501 tagctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     4561 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     4621 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     4681 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     4741 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     4801 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     4861 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     4921 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     4981 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     5041 gagcgtcgat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5101 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5161 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5221 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5281 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5341 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5401 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5461 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5521 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5581 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5641 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5701 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5761 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5821 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5881 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5941 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6001 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6061 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6121 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6181 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6241 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6301 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6361 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6421 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6481 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6541 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6601 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6661 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6721 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6781 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6841 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6901 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6961 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7021 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7081 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7141 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7201 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7261 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     7321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7381 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7441 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7501 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7561 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7621 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     7681 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     7741 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     7801 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     7861 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     7921 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     7981 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     8041 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     8101 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     8161 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     8221 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     8281 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     8341 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     8401 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     8461 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     8521 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     8581 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     8641 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     8701 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     8761 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     8821 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     8881 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     8941 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     9001 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     9061 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     9121 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     9181 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     9241 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     9301 cataatcagc cataccacat ttgtagagct tttacttgct ttaaaaaacc tcccacacct
     9361 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     9421 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9481 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     9541 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     9601 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     9661 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
