LOCUS       Exported               10569 bp ds-DNA     circular SYN 07-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     3'-LTR OK
            plasmid sequence between the RFA and WHV (after the removal of the 
            tTA2 sequnece from #1246)
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     3'UTR           1..634
                     /label=5'LTR
                     /note="5'LTR"
     misc_feature    1177..1678
                     /label=RRE
                     /note="RRE"
     misc_feature    join(2066..2076,4352..4369)
                     /label=RFA
                     /note="RFA [Split]"
     misc_feature    2094..3496
                     /label=rGluR1
     gene            3503..4333
                     /label=AcGFPnuc
                     /note="/regulatory_class=promoter"
     gene            3626..3644
                     /label=shGFP
                     /note="shGFP"
                     /note="/bound_moiety="
     gene            complement(3628..3644)
                     /label=shGFP
                     /note="shGFP"
     misc_signal     4240..4329
                     /label=NLS
                     /note="Tandem nuclear localization signal"
     gene            4241..4312
                     /label=nls (3x SV40)
                     /note="/nls sequence SV40 (3x)="
     enhancer        4506..5046
                     /label=WPRE
                     /note="WPRE sauvage"
     3'UTR           5125..5358
                     /label=3'LTR
                     /note="3'LTR"
     gene            6299..7159
                     /label=Ampicilline
                     /note="Ampicilline gene resistance"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacctgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgataa gcttgatatc gaattatcac aagtttgtac aaaaaagcag gctcctcact
     2101 gggtgtcttc atgagtgaat cccatggtca cttctgagtg tgaggacaga gggatgactc
     2161 tgggaagatg tttcagactg taaacatccc cagctgttat tgatggctgt cctacaggcc
     2221 ataccattat cctgtgcaca catctctctt gggtatctcc aagtcagctg tagtccagca
     2281 gggtccacgg aggcccagct tttcctggac acaaacaatc tgagatgtaa gtgaaagcag
     2341 cctgcatcct ccgactagaa ccagcgcaaa atgactcatg taattgccct gtgtgcgtac
     2401 cctgaatctt cttgttcacc cacccacaca actctgccgt tatagattcc tgcaccctgc
     2461 aagaaaaagg gagggggggg ggggacaagt tcaaatacct atggtttcaa aaaggccacc
     2521 cgtgcctctc ctaaagatag aagggtctgc cagcctccag gtccaagaag aggccagtta
     2581 cactgacgct actgctgcct gcctttctca gtcttccctt taaatttctg gagagggatt
     2641 tctagggtct cccccttggg aattagttgt aggaataatt gggccagtgg agtgtggaag
     2701 atgtatccag cgcaacccgt gcactactag agaaagtgac ctagttcaag cagctggtga
     2761 atccggggct attgcgggga ccccctgcca cctactgact tgcaactgaa cccgcattcc
     2821 caatagcatc ttatcccctg gcagggggag gggctgggag agaagcagag gaaggtgggg
     2881 gacgtgaggg ggacagtggg tttctccctt ttggggaaag caccctccac cttccatcct
     2941 tcctcccttc catcccctgc acccacaggg ggaaaaaagt gaggtgagga tggagaggca
     3001 ggggtgatga gttggggagg ccaatgcagg aattccgaga gctggctcct gggtatcagc
     3061 atagagagct ggcagcctgt gagagtgtga gggagagaga gagagagaga gagagagaga
     3121 gagagagaga gagagagaga gagagagaga gagagaaaca cgggagggtg agagaggaga
     3181 gaggctgcct gctagagaag aggaggagag cagagggaga gggggagcga gctagcgctc
     3241 caagcatgag gacgggctgc tcccggctca gttaatctgg ctgtcagtcg gtgttaacgc
     3301 tgcagttgaa ctgctcggct ccccttccaa gagaaacctc acggaaggaa gggaggaagg
     3361 aaagaagcaa gcaaggaact gcaggaagaa aagagccggc agagcatcaa gaagaatcga
     3421 agggagggga gggaagacca aatctatggt tggaccaggg cttctttttc gccaatgtaa
     3481 aaaggaatat gccgtagtcg acatggtgag caagggcgcc gagctgttca ccggcatcgt
     3541 gcccatcctg atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga
     3601 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3661 gctgcctgtg ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc
     3721 acgctacccc gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta
     3781 catccaggag cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt
     3841 gaagttcgag ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga
     3901 ggatggcaac atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat
     3961 catgaccgac aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga
     4021 ggatggcagc gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc
     4081 tgtgctgctg cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa
     4141 cgagaagcgc gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg
     4201 catggatgag ctgtacaagt ccggactcag atctcgagct gatccaaaaa agaagagaaa
     4261 ggtagatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg taggatccac
     4321 cggatctaga taaacccagc tttcttgtac acttgtacaa agtggtgata attcctgcag
     4381 cccgggggat ccactagttc taggatccac tagttctagt gatccaccat gtctagggaa
     4441 ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4501 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4561 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4621 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     4681 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4741 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4801 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     4861 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4921 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4981 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     5041 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5101 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5161 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5221 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5281 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5341 tgtggaaaat ctctagcagc atctagctag aattaattcc gtgtattcta tagtgtcacc
     5401 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
     5461 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
     5521 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
     5581 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
     5641 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     5701 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     5761 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     5821 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     5881 taatgcagga gtcgcataag ggagagcgtc gatatggtgc actctcagta caatctgctc
     5941 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     6001 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     6061 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     6121 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     6181 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6241 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6301 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6361 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6421 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6481 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6541 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6601 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6661 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6721 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6781 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6841 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6901 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6961 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7021 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7081 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7141 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7201 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7441 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     7741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8101 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
     8161 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8221 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8281 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8341 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8401 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8461 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
     8521 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     8581 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     8641 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     8701 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     8761 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
     8821 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     8881 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     8941 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     9001 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     9061 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     9121 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     9181 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     9241 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     9301 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
     9361 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     9421 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
     9481 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
     9541 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     9601 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     9661 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     9721 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     9781 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     9841 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     9901 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     9961 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
    10021 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
    10081 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
    10141 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga gcttttactt
    10201 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    10261 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10321 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10381 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    10441 cccaccccat accctattac cactgccaat tacctagtgg tttcatttac tctaaacctg
    10501 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10561 cttagtagt
//
