LOCUS       C53-H                   7878 bp ds-DNA     circular     06-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(55..816)
                     /label=54
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="57.3"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
                     /translation="TQRIVFLPDTQLPFEARKEMQAVIRFIGDVQPYGVVHIGDVLDLPQPSRWNRGTKGEFEGSVYRDADYAKKNLMEPLRKVYDGWIGMHEGNHDLRAREYLAKNAPALEGTHAFDIDVLLDFDGFGVELLPDFYDIAPGWISTHGHMGKMTLSQIAGSTALNGAKKFGKSVVCGHTHRQAVVSHSFGYGGSVRKTVTGMEVGHLMDMKKANYLKGGAGNWQMGFGMLTVDGKHVKAEIVPILGGKFTVDGQVWEV"
     CDS             complement(858..1532)
                     /label="mRFP1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPYDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTGA"
     promoter        complement(1772..1882)
                     /label="araBAD promoter (fragment)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="66.9"
                     /fragment="True"
                     /other="promoter"
     terminator      1899..1970
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      1986..2013
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        2088..2116
                     /label="J23119 promoter (fragment)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.6"
                     /match_length="82.9"
                     /fragment="True"
                     /other="promoter"
     CDS             2143..2856
                     /label="hfriFP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     terminator      2879..2950
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      2966..2993
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        3014..3042
                     /label="J23119 promoter (fragment)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.6"
                     /match_length="82.9"
                     /fragment="True"
                     /other="promoter"
     CDS             3069..3944
                     /label="araC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
     terminator      3969..4040
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      4056..4083
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        4104..4122
                     /label="J23119(SpeI) promoter (fragment)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="54.3"
                     /fragment="True"
                     /other="promoter"
     terminator      5682..5753
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      5769..5796
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.4"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     primer          complement(5957..5976)
                     /label="BBR"
                     /note="sequence: attaccgcctttgagtgagc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    6953..6958
                     /label="SacI"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          7739..7758
                     /label="BBF"
                     /note="sequence: tgccacctgacgtctaagaa"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(7815..7855)
                     /label="B0062"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 gcgaaaaaac cccgccgaag cggggttttt tgcgtactag agttattaac cggtgacttc
       61 ccagacctgg ccgtcaacgg tgaacttgcc tcccaggatc gggacgatct cagccttgac
      121 atgcttgccg tcgaccgtga gcatcccgaa gcccatctgc cagttcccgg ctccgccctt
      181 tagatagttg gccttcttca tgtccatcag gtgcccgact tccatgccgg tgacggtctt
      241 gcgcaccgag ccgccgtacc cgaacgagtg cgagacgaca gcctgccggt gcgtgtggcc
      301 gcagaccacg gacttgccga acttcttggc accgttgagc gctgtcgatc cggcgatctg
      361 ggatagcgtc atcttgccca tgtgcccgtg agtggagatc cagcccggag cgatgtcgta
      421 gaagtcaggc agcagctcca caccgaaccc gtcgaagtcg agcagcacgt cgatgtcgaa
      481 agcgtgcgta ccctccaggg ccggtgcgtt cttggccagg tactcgcggg ctcgcagatc
      541 gtggttgccc tcgtgcatcc cgatccagcc gtcgtagacc ttgcgcagcg gctccatcag
      601 gttcttcttg gcgtagtccg cgtcgcggta caccgagccc tcgaactcgc ccttggttcc
      661 ccggttccag cgcgagggct gcggcaggtc taggacgtca ccgatatgta ccacgccgta
      721 cggctggaca tccccgatga agcggatgac cgcttgcatc tctttgcgcg cctcgaaagg
      781 caactgagta tcgggtagaa agacgatacg ctgagtgccg gccatctagt atttctcctc
      841 ttttactaga gttattaagc accggtggag tgacgacctt cagcacgttc gtactgttca
      901 acgatggtgt agtcttcgtt gtgggaggtg atgtccagtt tgatgtcggt tttgtaagca
      961 cccggcagct gaaccggttt tttagccatg taggtggttt taacttcagc gtcgtagtga
     1021 ccaccgtctt tcagtttcag acgcattttg atttcacctt tcagagcacc gtcttccggg
     1081 tacatacgtt cggtggaagc ttcccaaccc atggtttttt tctgcataac cggaccgtcg
     1141 tacgggaagt tggtaccacg cagtttaact ttgtagatga actcaccgtc ttgcagggag
     1201 gagtcctggg taacggtaac aacaccaccg tcttcgaagt tcataacacg ttcccatttg
     1261 aaaccttccg ggaaggacag tttcaggtag tccgggatgt cagccgggtg tttaacgtaa
     1321 gctttggaac cgtactggaa ctgcggggac aggatgtccc aagcgaacgg cagcggacca
     1381 cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca
     1441 ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg
     1501 aactctttga taacgtcttc ggaggaagcc atctagtatt tctcctcttt tactagagcg
     1561 gccggcttgt cgacgacggc gcactcagtg gtgtacggta caaaccccga caatagcgag
     1621 gaggtaaatt gtaagcttta gggaaacgac atttactgac gcgactttct caaagctagg
     1681 gtgttggcct gaactgtacg cttggagttg taggacagac ataaaagtat cagatacgca
     1741 cactaaccat ctgctagccc aaaaaaacgg tatggagaaa cagtagagag ttgcgataaa
     1801 aagcgtcagg taggatccgc taatcttatg gactatcttg ctatggcata gcaaagtgtg
     1861 acgccgtgca aataatcaat gtctctagta ccaggcatca aataaaacga aaggctcagt
     1921 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1981 acactggctc accttcgggt gggcctttct gcgtttatag cccggatgat cctgacgacg
     2041 gagtgcgcgg tggttgacca gacaaaccac gactactaga gtttacggct agctcagtcc
     2101 taggtatagt gctagctact agagaaagag gagaaatact agatgcgtaa aggagaagaa
     2161 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     2221 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     2281 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
     2341 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
     2401 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     2461 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     2521 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     2581 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     2641 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     2701 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     2761 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     2821 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca
     2881 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2941 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     3001 actagagttt acggctagct cagtcctagg tacaatgcta gctactagag aaagaggaga
     3061 aatactagat ggctgaagcg caaaatgatc ccctgctgcc gggatactcg tttaacgccc
     3121 atctggtggc gggtttaacg ccgattgagg ccaatggtta tctcgatttt tttatcgacc
     3181 gaccgctggg aatgaaaggt tatattctca atctcaccat tcgcggtcag ggggtggtga
     3241 aaaatcaggg acgagaattt gtctgccgac cgggtgatat tttgctgttc ccgccaggag
     3301 agattcatca ctacggtcgt catccggagg ctcgcgaatg gtatcaccag tgggtttact
     3361 ttcgtccgcg cgcctactgg catgaatggc ttaactggcc gtcaatattt gccaatacgg
     3421 gtttctttcg cccggatgaa gcgcaccagc cgcatttcag cgacctgttt gggcaaatca
     3481 ttaacgccgg gcaaggggaa gggcgctatt cggagctgct ggcgataaat ctgcttgagc
     3541 aattgttact gcggcgcatg gaagcgatta acgagtcgct ccatccaccg atggataatc
     3601 gggtacgcga ggcttgtcag tacatcagcg atcacctggc agacagcaat tttgatatcg
     3661 ccagcgtcgc acagcatgtt tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc
     3721 agttagggat tagcgtctta agctggcgcg aggaccaacg catcagccag gcgaagctgc
     3781 ttttgagcac tacccggatg cctatcgcca ccgtcggtcg caatgttggt tttgacgatc
     3841 aactctattt ctcgcgagta tttaaaaaat gcaccggggc cagcccgagc gagttccgtg
     3901 ccggttgtga agaaaaagtg aatgatgtag ccgtcaagtt gtcataataa actactagag
     3961 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     4021 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     4081 gcgtttatat actagagttt acggctagct cagtcctagg tactatgcta gctactagag
     4141 tcacacagga aacctactag atgagagccc tggtagtcat ccgcctgtcc cgcgtcaccg
     4201 atgctacgac ttcaccggag cgtcagctgg agtattgcca gcagctctgc gcccagcgcg
     4261 gctgggacgt cgtcggggta gcggaggatc tggacgtctc cggggcggtc gatccgttcg
     4321 accggaagcg cagaccgaac ctggcccggt ggctagcgtt cgaggagcaa ccgtttgacg
     4381 tgatcgtggc gtaccgggta gatcggttga cccgatcgat ccggcatctt cagcagctgg
     4441 tccactgggc cgaggaccac aagaagctgg tcgtctccgc gaccgaagcg cacttcgata
     4501 cgacgacgcc gtttgcggcg gtcgtcatcg cgcttatggg aacggtggcg cagatggaat
     4561 tagaagcgat caaagagcgg aaccgttcgg ctgcgcattt caatatccgt gccgggaaat
     4621 accgaggatc cctgccgccg tggggatacc tgcctacgcg cgtggacggg gagtggcggc
     4681 tggtgccgga ccctgtgcag cgagagcgca tcctcgaggt gtatcaccgc gtcgtcgaca
     4741 accacgagcc gctgcatctg gtggcccacg acctgaaccg gcgtggtgtc ctgtcgccga
     4801 aggactactt cgcgcagctg caaggccgcg agccgcaggg ccgggagtgg tcggctaccg
     4861 cgctgaagcg atcgatgatc tccgaggcga tgctcgggta cgcgactctg aacggtaaga
     4921 ccgtccgaga cgacgacgga gccccgctgg tgcgggctga gccgatcctg acccgtgagc
     4981 agctggaggc gctgcgcgcc gagctcgtga agacctcccg ggcgaagccc gcggtgtcta
     5041 ccccgtcgct gctgctgcgg gtgttgttct gcgcggtgtg cggggagccc gcgtacaagt
     5101 tcgccggggg aggacgtaag cacccgcgct accgctgccg ctcgatgggg ttcccgaagc
     5161 actgcgggaa cggcacggtg gcgatggccg agtgggacgc gttctgcgag gagcaggtac
     5221 tggatctgct cggggacgcg gagcgtctgg agaaagtctg ggtagcgggc tcggactccg
     5281 cggtcgaact cgcggaggtg aacgcggagc tggtggacct gacgtcgctg gtcggctccc
     5341 cggcctaccg ggcgggctct ccgcagcgag aagcactgga tgcccgtatt gcggcgctgg
     5401 ccgcgcggca agaggagctg gagggcctgg aggctcgccc gtctggctgg gagtggcgcg
     5461 agaccgggca gcggttcggg gactggtggc gggagcagga caccgcggca aagaacacct
     5521 ggcttcggtc gatgaacgtt cggctgacgt tcgacgtccg cggcgggctg actcgcacga
     5581 tcgacttcgg ggatcttcag gagtacgagc agcatctcag gctcggcagc gtggtcgaac
     5641 ggctacacac cgggatgtcg taataaacta gagccaggca tcaaataaaa cgaaaggctc
     5701 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     5761 gtcacactgg ctcaccttcg ggttggcctt tctgcgttta tatactagta gcggccgctg
     5821 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
     5881 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccgatatca ggaaccgtaa
     6061 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
     6121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6481 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6841 ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     6901 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     6961 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     7021 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     7081 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     7141 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     7201 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     7261 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     7321 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     7381 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     7441 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     7501 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     7561 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     7621 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     7681 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg
     7741 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     7801 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     7861 tcgcggccgc ttctagag
//