LOCUS       pLLK012                 7360 bp    DNA     circular SYN 15-JUL-2025
DEFINITION  1856.
ACCESSION   1856
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     rep_origin      1..589
     CDS             952..1575
                     /note="Geneious type: cds"
     oriT            1951..2060
                     /note="pLannotate"
     misc_feature    2093..2464
     terminator      2744..2774
     CDS             complement(2808..3317)
                     /note="Geneious type: cds"
     RBS             3318..3339
                     /note="Geneious type: rbs"
     terminator      3791..3834
     CDS             4144..4680
                     /note="Geneious type: cds"
     CDS             4931..6568
                     /note="Geneious type: cds"
     rep_origin      6774..7013
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttcaaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgctcggc
      781 cggggatcga tccccgggta cgtacccggc agtttttctt tttcggcaag tgttcaagaa
      841 gttattaagt cgggagtgca gtcgaagtgg gcaagttgaa aaattcacaa aaatgtggta
      901 taatatcttt gttcattaga gcgataaact tgaatttgag agggaactta gatggtattt
      961 gaaaaaattg ataaaaatag ttggaacaga aaagagtatt ttgaccacta ctttgcaagt
     1021 gtaccttgta cctacagcat gaccgttaaa gtggatatca cacaaataaa ggaaaaggga
     1081 atgaaactat atcctgcaat gctttattat attgcaatga ttgtaaaccg ccattcagag
     1141 tttaggacgg caatcaatca agatggtgaa ttggggatat atgatgagat gataccaagc
     1201 tatacaatat ttcacaatga tactgaaaca ttttccagcc tttggactga gtgtaagtct
     1261 gactttaaat catttttagc agattatgaa agtgatacgc aacggtatgg aaacaatcat
     1321 agaatggaag gaaagccaaa tgctccggaa aacattttta atgtatctat gataccgtgg
     1381 tcaaccttcg atggctttaa tctgaatttg cagaaaggat atgattattt gattcctatt
     1441 tttactatgg ggaaatatta taaagaagat aacaaaatta tacttccttt ggcaattcaa
     1501 gttcatcacg cagtatgtga cggatttcac atttgccgtt ttgtaaacga attgcaggaa
     1561 ttgataaata gttaacttca ggtttgtctg taactaaaaa caagtattta agcaaaaaca
     1621 tcgtagaaat acggtgtttt ttgttaccct aaaatctaca attttataca taaccacagg
     1681 ttagtacaaa gaccttgtgt ttctttttga aaggcttaaa acaaggattt ttccttgatt
     1741 taagccccga aaagcaacac aaccaaggtt ttagtatcaa tctgtggttt ttatattttc
     1801 agagaccggt cagatctgca gaattcgccc ttcctgcttc ggggtcatta tagcgatttt
     1861 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga
     1921 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc
     1981 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc
     2041 tgctctgcga ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctga
     2101 tgaaaccaag ccaaccagga agggcagccc acctatcaag gtgtactgcc ttccagacga
     2161 acgaagagcg attgaggaaa aggcggcggc ggccggcatg agcctgtcgg cctacctgct
     2221 ggccgtcggc cagggctaca aaatcacggg cgtcgtggac tatgagcacg tccgcgagct
     2281 ggcccgcatc aatggcgacc tgggccgcct gggcggcctg ctgaaactct ggctcaccga
     2341 cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc gccctgctgg cgaagatcga
     2401 agagaagcag gacgagcttg gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc
     2461 atgacttttt tagccgctaa aacggccggg gggtgcgcgt gattgccaag cacgtcccca
     2521 tgcgctccat caagaagagc gacttcgcgg agctggtgaa gtacatcacc gacgagcaag
     2581 gcaagaccga tcccctacta ctgacagctt ccaaggagct aaagaggtcc ctagcgcagc
     2641 ctaagggcga attccagcac actggcatct ttttatttag ggatttctca cataaaatag
     2701 aggctattta tagcctctat tttattttat atttatttta aataaaaaag acttctcatg
     2761 agagaagcct ttttctattt aaagttttat taaaacttat aggatcccta tagaatttct
     2821 tcaaaaagtc tataacctgt aacactgttt atagttactc taaacaacat agatccatca
     2881 ggtgtaatta acctttcatc tattatttta tttccattcc acaaagtgcc tgttacggtt
     2941 attttctttc catcaaaaac tgcaatccct tcgtatggtc taccaaaata atttaacata
     3001 tttggtgtta caccatcaat tactaaagtt ccataaggta atattacttt aaagtgatga
     3061 tcgtctactg gatatacaac tttgaatact tcctcaattt gtgccatttg gtctgctgat
     3121 agtccctcat aaggaattat tacatgtata tcgatcttta atgcattttc tccacttcta
     3181 actattcttt gtattggtgt aactgagact gcaagatttt gtaaaagaga actcactcca
     3241 ccctgttcta aaacttgatc taaattatat gcagcagttt gttcccaatc acccacaaaa
     3301 tcttcaagtg taaacacaaa attttctcct ttactgcagg agctccatta tattaaaatt
     3361 acatctcttc attatgtaca tagaataatc atccatatat tcctaatatt tctcacatct
     3421 ctataataat ccatttttat tctttttgca agtcattttt ttgaaattat tatttttccc
     3481 aagcatatga gtttttatac ccttaaactc caatatttaa taacttaaaa aatttgattt
     3541 tacttattta aattacttat ttaaaaattc ttgtgtagca tacacttctc cattaggcaa
     3601 tatatataat ccaaacccag tatatacaaa atcactattt aacatatttt cattatattt
     3661 atctgacttt ttccaaactg taaatattac atttgctact tctctagcat cctttgttgt
     3721 ataagtactt tttgtaacaa gtggtacaaa agctatattt tcttcacttc tcaagccata
     3781 tccagggtac cataaaaata agaagcctgc aaatgcaggc ttcttatttt tatgctagct
     3841 tgatgcagaa ttcgccctta agtctaaaaa ttaggggaga tgtaaggatt tgggaaaaat
     3901 agaagatgtt ataatcataa atatggtatt cgtaggctta aagtcaaaaa ggaggtgaaa
     3961 tataaataga tttttagcta aattaagtaa gaaataggag gagatttatt gaacaaaaaa
     4021 ttagaaaaac catttgtata taagagagag tacgatttga ctggatatga tgttgaaatt
     4081 ttacaaaaat atgagttaga acaagcaata tatgtttatg ttgggagtag ttgtgcatat
     4141 aacatgagag ctagaagtag taaatggaga taccatataa gaacaaataa taagtctata
     4201 tgttgtaaca ttaaaaattt tatacataac ttggaattgt tttataaaat ggaattaaag
     4261 ttgtcagata atattattaa tgataagcta tactatagca atatagcaga gtttgaagaa
     4321 tttgaaacac tagaaaaagc tagagaggta gaaagtacta ttataagtca atatcaattt
     4381 ttagattcta taaatcatat gttaaaacaa aaaataattt tattgagtaa taaggatagt
     4441 gtgttaaaca taactaaaaa tggaaataca aattatttga aagtaaaaaa taaatacata
     4501 gaaaaacata agaacaagcc aataatgaga taccatatca actgtcaatt caatacagat
     4561 ggaagtgtca aaagtattac acaggagttt gaaccaatat tggaattaaa caaaaaaaat
     4621 accctaagcc gaccaagcag agtattttta aaataatatt ttaagataac aacaaaatga
     4681 gataatacta ctagacaatg acaactcaac taccaattga gtttatggag ctaccaactc
     4741 caatatcggt ctaactgatt aagtatctgt agttatataa taatattgct atcaatttta
     4801 gcatcttaac aatattatta tacatactaa gctaaaatta ttcaatagtt gtaaaagttg
     4861 attagtcaat aagtatatat ttaatgtagt gttatctctt aaaaaaacta gataaggaga
     4921 taataaatat atggaacaat tagattcaaa atataagttg aaaaaatttc taatggcagt
     4981 atttagagat ggtataggac aaggaaataa tcttattgat aatgaatatg ttagagtatt
     5041 tcaaaataat aaaagtaata gtaaacaatt agaactcgga gaagaattta aagaatatag
     5101 taaaacaact ttttttaaaa atatagatga tatagtagaa tttaccttcg caaaaaatat
     5161 ttattatgaa aatacatttt ttaacctatg tacaactgat ggaaaagcag gaaccaatga
     5221 aaacttaata aatagatatg cattaggatt tgattttgac aaaaaagaat taggacaagg
     5281 ttttaattat aaagatataa ttaatttatt tactaagata ggattacatt atcatatcct
     5341 agttgatagt ggaaatggat tccatgttta tgtgctaatt aataaaacta ataacattaa
     5401 gttagtatca gaagttacaa atacattaat aaataaattg ggtgcagata aacaagcaaa
     5461 tttatctact caagtattaa gagtacctta tacatataat attaaaaata ctactaaaca
     5521 agtaaaaata atacaccaag acaaaaatat atatagatat gacatagaaa agttagctaa
     5581 aaaatattgc aaagatgtaa aaacagtagg taatactaat acaaaatata tattagatag
     5641 taagctacca aattgtatag tagatatttt aaaaaatggt agtaaagatg gacataaaaa
     5701 cctagatttg caaaaaatag ttgtgacttt aagattgagg aataaaagtt taagtcaagt
     5761 aatatccgtt gctagagaat ggaactatat atcacaaaat agtctttcaa atagtgagct
     5821 agaatatcaa gtcaagtata tgtatgagaa gcttaaaacg gttaattttg gttgtacagg
     5881 ttgtgagttt aatagtgatt gttggaataa aatagaatca gattttatat atagtgatga
     5941 agatactttg ttcaatatgc cacataagca ctcaaaggat ttgaaatata agaataggaa
     6001 aggggttaaa ataatgactg gtaatcaatt gtttatctat aatgtgttac ttaacaataa
     6061 agatagagaa ttaaacatag acgatataat ggagctgata acctataaac gtaagaagaa
     6121 agttaaaaac attgttatga gtgaaaagac attaagagaa acattaaaag aacttcaaca
     6181 taatgattat attacaaaaa caaaaggtgt tacaaagcta ggaataaaag atacatacaa
     6241 tgtaaaagaa gttagatgta atatagataa acaatatact attagttact ttgttaccat
     6301 ggcagtaatt tggggaataa tttcaactga agaattaaga ttatatactc atatgagata
     6361 taagcaagat ttattggtca aagatgataa aataaaagga aatatattaa gaattaatca
     6421 agaggaatta gcaaaagatt taggagtaac acagcaaaga atttcaaata tgatagaatc
     6481 tttattagat actaaaattt tagatgtatg ggaaactaaa ataaatgata gaggatttat
     6541 gtactataca tatagattaa acaagtagat ttttgatagg attagaattg attttctagt
     6601 cctattttta tgcaaaaaaa ctaattaata aaaatttctt ttggtaaaat aattgtacga
     6661 gaattgcaaa aaaaaaatgg catcaaagta ttgaaattaa gccgttttaa aaatttcttt
     6721 tggtaaaata attgtacata tatatgtagt atatatatat atgtttttta gagaatgtat
     6781 aactagaata tagagctaga atatagagaa tgtataacta gaatatagag ctagaatata
     6841 gagaatgtat aactagaata tagagctaga atatagagaa tgtataacta gaatatagag
     6901 ctagaatata gagaatgtat aactagaata tagagctaga atatagagaa tgtataacta
     6961 gaatatagag ctagaatata gagaatgtat aactagaata tagagctaga atcctaatta
     7021 gtaggtgctt ttttaaaaca agttaaaaat caaaaatagt attagtaagc attggaaatg
     7081 ctagattcta aaatagaaaa gtaaaaaatt ggtgcactat ctaaacttat ctatatcgct
     7141 ttttccgtcg tttggttctc tagttacgat acaggggata tgcttatatt gagttatagt
     7201 actaatcagt gcttaatata gttaataaaa ttatagttac catagtttag taactatgat
     7261 gtatgttagt tagaaacttg catttcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7321 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
