LOCUS       pJAK081                11185 bp    DNA     circular SYN 15-JUL-2025
DEFINITION  1609.
ACCESSION   1609
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     rep_origin      1..589
     oriT            910..1019
                     /note="pLannotate"
     CDS             1052..1423
                     /note="pLannotate"
     terminator      1566..1619
                     /note="derived from CD0164 gene of Clostridium difficile
                     630"
     CDS             1875..3158
                     /note="pLannotate"
     terminator      3421..3464
     CDS             complement(5129..5632)
                     /note="orf2"
                     /codon_start=1
                     /transl_table=11
                     /product="putative plasmid replication protein"
                     /protein_id="AFL93717.1"
                     /db_xref="GI:390517384"
                     /note="Geneious type: cds"
     CDS             5956..6579
                     /gene="catP"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="AFL93718.1"
                     /db_xref="GI:390517385"
     primer_bind     6526..6552
     misc_feature    6656..7855
                     /note="Geneious type: motif"
     terminator      7876..7919
     CDS             complement(7928..8551)
                     /note="Geneious type: cds"
     misc_feature    8573..8591
                     /note="Geneious type: operator"
     misc_feature    8658..8676
                     /note="Geneious type: operator"
     CDS             8766..9827
                     /transl_table=11
                     /gene="mreB2"
                     /locus_tag="CD630_11450"
                     /old_locus_tag="CD1145"
                     /product="Rod shape-determining protein MreB"
                     /note="Evidence 2a : Function of homologous
                     geneexperimentally demonstrated in an other organism;
                     Product type s : structure; PubMedId: 18291013, 18283557,
                     17981078, 17981077,17980647,17563102, 17434308, 17347523"
                     /db_xref="GOA:Q18B05; InterPro:IPR001023;
                     InterPro:IPR004753; UniProtKB/TrEMBL:Q18B05"
                     /inference="ab initio prediction:AMIGene:2.0"
                     /protein_id="CAJ67998.1"
     terminator      9861..9891
     misc_feature    9896..11095
                     /note="Geneious type: motif"
     primer_bind     complement(11153..11174)
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcagggcc ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt
      841 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg
      901 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac
      961 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc
     1021 taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa
     1081 gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa
     1141 ggcggcggcg gccggcatga gcctgtcggc ctacctgctg gccgtcggcc agggctacaa
     1201 aatcacgggc gtcgtggact atgagcacgt ccgcgagctg gcccgcatca atggcgacct
     1261 gggccgcctg ggcggcctgc tgaaactctg gctcaccgac gacccgcgca cggcgcggtt
     1321 cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa gagaagcagg acgagcttgg
     1381 caaggtcatg atgggcgtgg tccgcccgag ggcagagcca tgactttttt agccgctaaa
     1441 acggccgggg ggtgcgcgtg attgccaagc acgtccccat gcgctccatc aagaagagcg
     1501 acttcgcgga gctggtgaag tacatcaccg acgagcaagg caagaccgat cgggccccct
     1561 gcaggataaa aaaattgtag ataaatttta taaaatagtt ttatctacaa tttttttatc
     1621 aggaaacagc tatgaccgcg gccgctgtat ccatatgacc atgattacga attccccgga
     1681 tcgagatagt atatgatgca tattctttaa atatagataa agttatagaa gcaatagaag
     1741 atttaggatt tactgtaata taaattacac ttttaaaaag tttaaaaaca tgatacaata
     1801 agttatggtt ggaattgtta tccgctcaca attccaactt atgattaaaa ttttaaggag
     1861 gtgtatttca taatgtgtcg aataacgctt tacaaacaat tattaacgcc cggttaccag
     1921 gcgaagaggg gctgtggcag attcatctgc aggacggaaa aatcagcgcc attgatgcgc
     1981 aatccggcgt gatgcccata actgaaaaca gcctggatgc cgaacaaggt ttagttatac
     2041 cgccgtttgt ggagccacat attcacctgg acaccacgca aaccgccgga caaccgaact
     2101 ggaatcagtc cggcacgctg tttgaaggca ttgaacgctg ggccgagcgc aaagcgttat
     2161 taacccatga cgatgtgaaa caacgcgcat ggcaaacgct gaaatggcag attgccaacg
     2221 gcattcagca tgtgcgtacc catgtcgatg tttcggatgc aacgctaact gcgctgaaag
     2281 caatgctgga agtgaagcag gaagtcgcgc cgtggattga tctgcaaatc gtcgccttcc
     2341 ctcaggaagg gattttgtcg tatcccaacg gtgaagcgtt gctggaagag gcgttacgct
     2401 taggggcaga tgtagtgggg gcgattccgc attttgaatt tacccgtgaa tacggcgtgg
     2461 agtcgctgca taaaaccttc gccctggcgc aaaaatacga ccgtctcatc gacgttcact
     2521 gtgatgagat cgatgacgag cagtcgcgct ttgtcgaaac cgttgctgcc ctggcgcacc
     2581 atgaaggcat gggcgcacga gtcaccgcca gccacaccac ggcaatgcac tcctataacg
     2641 gggcgtatac ctcacgcctg ttccgcttgc tgaaaatgtc cggtattaac tttgtcgcca
     2701 acccgctggt caatattcat ctgcaaggac gtttcgatac gtatccaaaa cgtcgcggca
     2761 tcacgcgcgt taaagagatg ctggagtccg gcattaacgt ctgctttggt cacgatgatg
     2821 tcttcgatcc gtggtatccg ctgggaacgg cgaatatgct gcaagtgctg catatggggc
     2881 tgcatgtttg ccagttgatg ggctacgggc agattaacga tggcctgaat ttaatcaccc
     2941 accacagcgc aaggacgttg aatttgcagg attacggcat tgccgccgga aacagcgcca
     3001 acctgattat cctgccggct gaaaatgggt ttgatgcgct gcgccgtcag gttccggtac
     3061 gttattcggt acgtggcggc aaggtgattg ccagcacaca accggcacaa accaccgtat
     3121 atctggagca gccagaagcc atcgattaca aacgttgaac gactgggtta cagcgagctt
     3181 agtttaaagg gcgaattcct agagtcgacg tcacgcgtcc atggagatct cgaggcctgc
     3241 agacatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3301 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3361 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgctag
     3421 cataaaaata agaagcctgc atttgcaggc ttcttatttt tatggcgcgc cgttctgaat
     3481 ccttagctaa tggttcaaca ggtaactatg acgaagatag caccctggat aagtctgtaa
     3541 tggattctaa ggcatttaat gaagacgtgt atataaaatg tgctaatgaa aaagaaaatg
     3601 cgttaaaaga gcctaaaatg agttcaaatg gttttgaaat tgattggtag tttaatttaa
     3661 tatatttttt ctattggcta tctcgatacc tatagaatct tctgttcact tttgtttttg
     3721 aaatataaaa aggggctttt tagccccttt tttttaaaac tccggaggag tttcttcatt
     3781 cttgatacta tacgtaacta tttcgatttg acttcattgt caattaagct agtaaaatca
     3841 atggttaaaa aacaaaaaac ttgcattttt ctacctagta atttataatt ttaagtgtcg
     3901 agtttaaaag tataatttac caggaaagga gcaagttttt taataaggaa aaatttttcc
     3961 ttttaaaatt ctatttcgtt atatgactaa ttataatcaa aaaaatgaaa ataaacaaga
     4021 ggtaaaaact gctttagaga aatgtactga taaaaaaaga aaaaatccta gatttacgtc
     4081 atacatagca cctttaacta ctaagaaaaa tattgaaagg acttccactt gtggagatta
     4141 tttgtttatg ttgagtgatg cagacttaga acattttaaa ttacataaag gtaatttttg
     4201 cggtaataga ttttgtccaa tgtgtagttg gcgacttgct tgtaaggata gtttagaaat
     4261 atctattctt atggagcatt taagaaaaga agaaaataaa gagtttatat ttttaactct
     4321 tacaactcca aatgtaaaaa gttatgatct taattattct attaaacaat ataataaatc
     4381 ttttaaaaaa ttaatggagc gtaaggaagt taaggatata actaaaggtt atataagaaa
     4441 attagaagta acttaccaaa aggaaaaata cataacaaag gatttatgga aaataaaaaa
     4501 agattattat caaaaaaaag gacttgaaat tggtgattta gaacctaatt ttgatactta
     4561 taatcctcat tttcatgtag ttattgcagt taataaaagt tattttacag ataaaaatta
     4621 ttatataaat cgagaaagat ggttggaatt atggaagttt gctactaagg atgattctat
     4681 aactcaagtt gatgttagaa aagcaaaaat taatgattat aaagaggttt acgaacttgc
     4741 gaaatattca gctaaagaca ctgattattt aatatcgagg ccagtatttg aaatttttta
     4801 taaagcatta aaaggcaagc aggtattagt ttttagtgga ttttttaaag atgcacacaa
     4861 attgtacaag caaggaaaac ttgatgttta taaaaagaaa gatgaaatta aatatgtcta
     4921 tatagtttat tataattggt gcaaaaaaca atatgaaaaa actagaataa gggaacttac
     4981 ggaagatgaa aaagaagaat taaatcaaga tttaatagat ggaatagaaa tagattaaag
     5041 tgtaactata ctttatatat atatgattaa aaaaataaaa aacaacagcc tattaggttg
     5101 ttgtttttta ttttctttat taattttttt aatttttagt ttttagttct tttttaaaat
     5161 aagtttcagc ctctttttca atatttttta aagaaggagt atttgcatga attgcctttt
     5221 ttctaacaga cttaggaaat attttaacag tatcttcttg cgccggtgat tttggaactt
     5281 cataacttac taatttataa ttattatttt cttttttaat tgtaacagtt gcaaaagaag
     5341 ctgaacctgt tccttcaact agtttatcat cttcaatata atattcttga cctatatagt
     5401 ataaatatat ttttattata tttttacttt tttctgaatc tattatttta taatcataaa
     5461 aagttttacc accaaaagaa ggttgtactc cttctggtcc aacatatttt tttactatat
     5521 tatctaaata atttttggga actggtgttg taatttgatt aatcgaacaa ccagttatac
     5581 ttaaaggaat tataactata aaaatatata ggattatctt tttaaatttc attattggcc
     5641 tcctttttat taaatttatg ttaccataaa aaggacataa cgggaatatg tagaatattt
     5701 ttaatgtaga caaaatttta cataaatata aagaaaggaa gtgtttgttt aaattttata
     5761 gcaaactatc aaaaattagg gggataaaaa tttatgaaaa aaaggttttc gatgttattt
     5821 ttatgtttaa ctttaatagt ttgtggttta tttacaaatt cggccggcca gtgggcaagt
     5881 tgaaaaattc acaaaaatgt ggtataatat ctttgttcat tagagcgata aacttgaatt
     5941 tgagagggaa cttagatggt atttgaaaaa attgataaaa atagttggaa cagaaaagag
     6001 tattttgacc actactttgc aagtgtacct tgtacctaca gcatgaccgt taaagtggat
     6061 atcacacaaa taaaggaaaa gggaatgaaa ctatatcctg caatgcttta ttatattgca
     6121 atgattgtaa accgccattc agagtttagg acggcaatca atcaagatgg tgaattgggg
     6181 atatatgatg agatgatacc aagctataca atatttcaca atgatactga aacattttcc
     6241 agcctttgga ctgagtgtaa gtctgacttt aaatcatttt tagcagatta tgaaagtgat
     6301 acgcaacggt atggaaacaa tcatagaatg gaaggaaagc caaatgctcc ggaaaacatt
     6361 tttaatgtat ctatgatacc gtggtcaacc ttcgatggct ttaatctgaa tttgcagaaa
     6421 ggatatgatt atttgattcc tatttttact atggggaaat attataaaga agataacaaa
     6481 attatacttc ctttggcaat tcaagttcat cacgcagtat gtgacggatt tcacatttgc
     6541 cgttttgtaa acgaattgca ggaattgata aatagttaac ttcaggtttg tctgtaacta
     6601 aaaacaagta tttaagcaaa aacatcgtag aaatacggtg ttttttgtta ccctaaatat
     6661 atcttgccca aatgtcaaag ctggtggaat ggcttttggt ataaaaaatg aagtggcaag
     6721 agaagttgtt cgtgaggtaa gaaacataac gaaactgcct ttagtaataa aattatcacc
     6781 caatgcagaa gatattgtag gaatggcaaa agtttgtgaa gaagaaggtg ctgatggagt
     6841 ttcattagtt aatactttta aagcaatggc aattgatata aaaaatagaa gaccagtatt
     6901 tgaaaatgta tatgctggat tatctggacc agctataaaa cctatagctt tgagaatggt
     6961 acatgaagtg tgtaaaaatg taaatatacc tgtaatggga atgggcggaa taactaaagc
     7021 cacagatgct atagagttta taatggcagg agctacttgt atccaagttg gaactgcaaa
     7081 ttttatgaat cctagaatag gtattgaaat aatagatggg attaatgaat ttatggatag
     7141 agaaggaata aaaagtttag acgaaataag aggaattata taaggtagaa taaatagtat
     7201 tttaaagata aaaaatatag attaaataaa caataatttt ataacattaa catggaggaa
     7261 aaataatatg agtaaagtag atgtagtgga tatattaaag aaaagtgatg cattgttgga
     7321 gggacatttt ttattatctt caggaaaaca cagtaataga tacgtacaat gtgcaaaagt
     7381 attaagattt ccagaatatg ctgctgaggt attaagtaca gttgttgaac aaataaaaga
     7441 cttagatata gacttagtag taggaccagc tatgggtgga gtaatagttt cttatgagtt
     7501 aggaagacaa ttaggaaaag aagctgtatt tactgagaga aaagacaata caatggagtt
     7561 aagaagagga tttgaagtta aaaaaggagc aaagataata attgctgaag acgttgtaac
     7621 tactggtaaa tcaactatgg agacaaaaag agtattagaa gccttaggtg gagaagttgt
     7681 aggtgttgca tgtatagcag atagaactaa tcatgatata ggtatgccta tatatagtgc
     7741 tataaaactt gatattcaag tttatgaatc tgatgagtgt cctttatgta aggaaggaaa
     7801 attaccagtt gttaagcctg gaagtagaga gttcaaggaa ttagggatgt aataacgaat
     7861 tctgcatcaa gctagcataa aaataagaag cctgcatttg caggcttctt atttttatgg
     7921 taccttaaga cccactttca catttaagtt gtttttctaa tccgcatatg atcaattcaa
     7981 ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata gcttgtcgta
     8041 ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg acttgatgct
     8101 cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt gcatataatg
     8161 cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg agagtttcat
     8221 actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga tgacttagta
     8281 aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta
     8341 aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg tcgagcaaag
     8401 cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc ttctgggcga
     8461 gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat gcgctgttaa
     8521 tcactttact tttatctaat ctagacatca ttaattcctc ctttttgttg acattatatc
     8581 attgatagag ttatttgtca aactagtttt ttatttcgat gccctggact tcatgaaaaa
     8641 ctaaaaaaaa tattgacact ctatcattga tagagtataa ttaaaataag cttgatcgta
     8701 gcgttaacag atctgagctc agaatgtttt aaacatgatt cttaatagta ggaaggagta
     8761 aaatcatggc taaggaaaaa aagaaagaaa aaaaaggttt ttttagtttt aataagatga
     8821 caaaggatat gggaatagat ttagggactg caaatacatt agtttacata aaaggacaag
     8881 gtattgttgt tagagaacca tcagttgtcg caataagaga tgatagtaaa gaggtattag
     8941 cagtaggtga agaagccaaa aaaatgatag gtagaacacc aggaaatata gtagctataa
     9001 gacctatgaa agatggagta atagctgatt ttgacataac tcaatcaatg attagctatt
     9061 ttatacaaaa agcggctgat aaaaaaggtg ttgtaagtcc aagaatagct atatgtgttc
     9121 cttttggagt tacagaagta gaaaagagag ctatagaaga ggctgcaaga caagcaggag
     9181 ctaaagatgc attccttata gaagagccaa tggcagcggc tataggggct ggattaaaag
     9241 ttgaagaacc agaaggtaat atggtagttg atattggtgg aggtacttca gaaatagctg
     9301 taatatcttt aggaggaatc gttactgcta aatctataag aataggtgga gacgaatttg
     9361 acgagtctat agtggcttat gtaaaaaaag aatataatct tatgataggt gaaagaactg
     9421 ctgaaaatgt aaaaattaac ataggttcta catttaaaga tgatgaagaa ataaatatgc
     9481 aaataagagg tagagattta atctcaggat tacctaaaac aatagaaata tgctctacag
     9541 aagttagaga agcattaaaa gaacctgtaa gttcaatagt agatgcaata aaatctacac
     9601 tagaaagaac tccaccagaa ttagcctcag atataatgga aaacggaata atgctaacag
     9661 gaggaggcgc tttgcttaga ggtcttgata agcttattac acaggaaact ggtatgtctg
     9721 tacaaatagc agaaacacct cttgattgtg ttgcacttgg aacaggtaaa tctgttgaag
     9781 accaagaaat ctttgaaaaa gtattaatga tgaatactaa gaattaggga tcctataagt
     9841 tttaataaaa ctttaaatag aaaaaggctt ctctcatgag aagtcttttt tatttaaact
     9901 taattattta tagtgttact taaaaaatgt aaatttttta gagtaaaaat ttgtaatata
     9961 acatataact taaaaataaa aagcagttag ccttataaat gaaggttagc tgtttttttt
    10021 gttaaaaata tatttacaaa ataagagata gtagcagtac aatataagta taaggtaaaa
    10081 gtattatata ttagggaggg gtaagtatga aaaatcaatt aaatgaaaat cataaaaagg
    10141 ttgtattttt tggagtaggt gctgtaggag ctacttttgc agaacaattt tttaactcta
    10201 aatatgattt taaaattctt tgtgataatg aaagaaagaa aagatattta gaagaaggat
    10261 ttataataaa tggaaaaagg tatgatttta attatgtaac taaagatgag tataaacaag
    10321 aggctgattt tataattata ggtctaaaat ataataattt aaaagaaaat ataaaagaat
    10381 tagatggatt agttggaaaa aatacagtta taatgtctct gctaaatgga gttgatagcg
    10441 aagagataat aggagaaaga tttggaattg gaaaaatggt atattcatat gttaccaata
    10501 tagatgcaaa gaaaatcaat aataatatta tacacactac taatgggata attgtatttg
    10561 gtaacaaaga taatagtgaa gatagaaaaa ctaatataat aactgaagtc tttgatgatg
    10621 taaatataga atatatttta tcaaaagata ttcaacgaga tatgtggtgg aagtacatgg
    10681 ttaatattgg tgtaaatcaa acttcagcta tacttggtgc accttatgga gtttttcaga
    10741 gttctgagca tttaagagaa ttagcaaaat ctgcaatgag ggaagttgtt gctatagcac
    10801 aagcaaaaga catatctctt acagaagatg atgttgaaaa ttcattacat agaatactag
    10861 aacattcaaa agaaggaaga acatcaatgc ttcaagacgt ggaagctcat agacctacag
    10921 aagtagatat gttttctaag aatatctgca aacttggaaa aaaatatagc atacctactc
    10981 ctataaatca gactttattt tatatgataa aagtaattga aagtagattt taaagggtat
    11041 ttgtaagaaa tattgataat tattgatttt aaattttatt aatgttatat actatccttt
    11101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11161 ccgtagaaaa gatcaaagga tcttc
//
