LOCUS       Exported                6901 bp ds-DNA     circular SYN 21-FEB-2023
DEFINITION  pWJ1781. Yeast centromere vector pRS415 with LEU2/AmpR markers, 
            galactose inducible promoter (GAL1,10), and A/B homology sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 18, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..1148
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/locus_tag=source:synthetic DNA construct"
     source          2299..6013
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /label=source:synthetic DNA construct(1)
                     /note="/locus_tag=source:synthetic DNA construct(1)"
     terminator      1..326
                     /locus_tag="ADH2 terminator"
                     /label=ADH2 terminator
                     /note="ADH2 terminator;S. cerevisiae"
     primer_bind     complement(33..54)
                     /locus_tag="ADH2t-VR"
                     /label=ADH2t-VR
                     /note="ADH2t-VR;pWJ1512-R"
     rep_origin      651..957
                     /locus_tag="f1 origin"
                     /label=f1 origin
                     /note="f1 origin"
     misc_feature    1149..1364
                     /locus_tag="URA3 Promoter"
                     /label=URA3 Promoter
     misc_feature    1365..2168
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    2169..2298
                     /locus_tag="Possible URA3 Term"
                     /label=Possible URA3 Term
     misc_feature    2549..3067
                     /locus_tag="CEN6/ARS4"
                     /label=CEN6/ARS4
                     /note="CEN6/ARS4;S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence (ARS4)"
     promoter        3129..3157
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="AmpR promoter"
     gene            3199..4059
                     /gene="AmpR"
                     /locus_tag="AmpR"
                     /label=AmpR
     CDS             3199..4059
                     /codon_start=1
                     /gene="AmpR"
                     /locus_tag="AmpR(1)"
                     /label=AmpR
                     /label=AmpR(1)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4214..4833
                     /locus_tag="pBR322 origin"
                     /label=pBR322 origin
                     /note="pBR322 origin"
     promoter        5336..6005
                     /gene="GAL1,10 promoter"
                     /locus_tag="GAL1,10 promoter"
                     /label=GAL1,10 promoter
                     /note="Bi-directional promoter for S. cerevisiae GAL1 and
                     GAL10"
     primer_bind     5974..5995
                     /locus_tag="GAL1p-VF"
                     /label=GAL1p-VF
                     /note="GAL1p-VF;pWJ1781-VF"
     misc_feature    6014..6901
                     /locus_tag="HIS6 SRCKD"
                     /label=HIS6 SRCKD
ORIGIN
        1 aagctttgga cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt
       61 ctaccttgcc agaaatttac gaaaagatgg aaaagggtca aatcgttggt agatacgttg
      121 ttgacacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
      181 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
      241 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
      301 ctcttattga ccacacctct accggcatgc accattcctt gcggcggcgg tgctcaacgg
      361 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
      421 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
      481 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      541 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      601 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
      661 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      721 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      781 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      841 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      901 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      961 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     1021 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     1081 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     1141 atcgacggtt caattcatca tttttttttt attctttttt ttgatttcgg tttccttgaa
     1201 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta
     1261 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa
     1321 cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat
     1381 ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc
     1441 acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg
     1501 agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga
     1561 ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt
     1621 ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact
     1681 ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg
     1741 gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag
     1801 gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta
     1861 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa
     1921 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt
     1981 tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta
     2041 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg
     2101 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc
     2161 aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt
     2221 caatttaatt atatcagtta ttaccctatg cggtgtgaaa taccgcacag atgcgtaagg
     2281 agaaaatacc gcatcaggta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     2341 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     2401 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     2461 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     2521 ttaatgtcat gataataatg gtttcttagg acggatcgct tgcctgtaac ttacacgcgc
     2581 ctcgtatctt ttaatgatgg aataatttgg gaatttactc tgtgtttatt tatttttatg
     2641 ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa
     2701 aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat
     2761 tagacaagaa aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa
     2821 tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata
     2881 cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt
     2941 ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt
     3001 taatttatat atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa
     3061 aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3421 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3901 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3961 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4021 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4081 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4141 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4201 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4261 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4321 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4381 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4441 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4501 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4561 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4621 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4681 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4741 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4801 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4861 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4921 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4981 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5041 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5101 gcaattaatg tgagttacct cactcattag gcaccccagg ctttacactt tatgcttccg
     5161 gctcctatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     5221 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac
     5281 cgcggtggcg gccggccgct ctcgcgacac gaggcccttt cgtcttcaag aattcgaatt
     5341 ttcaaaaatt cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat
     5401 ggcattacca ccatatacat atccatatac atatccatat ctaatcttac ttatatgttg
     5461 tggaaatgta aagagcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag
     5521 taatacgctt aactgctcat tgctatattg aagtacggat tagaagccgc cgagcgggtg
     5581 acagccctcc gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg
     5641 aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct
     5701 tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaatgaac
     5761 gaatcaaatt aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg
     5821 taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat gcaaaaactg
     5881 cataaccact ttaactaata ctttcaacat tttcggtttg tattacttct tattcaaatg
     5941 taataaaagt atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa
     6001 aaaccccgga tccatgggca gcagccatca tcatcatcat cacgattacg atatcccaac
     6061 gaccgaaaac ctttacttcc agggccatat gaaggatgcc tgggagatcc ctcgggagtc
     6121 gctgcggctg gaggtcaagc tgggccaggg ctgctttggc gaggtgtgga tggggacctg
     6181 gaacggtacc accagggtgg ccatcaaaac cctgaagcct ggcacgatgt ctccagaggc
     6241 cttcctgcag gaggcccagg tcatgaagaa gctgaggcat gagaagctgg tgcagttgta
     6301 tgctgtggtt tcagaggagc ccatttacat cgtcacggag tacatgagca aggggagttt
     6361 gctggacttt ctcaaggggg agacaggcaa gtacctgcgg ctgcctcagc tggtggacat
     6421 ggctgctcag atcgcctcag gcatggcgta cgtggagcgg atgaactacg tccaccggga
     6481 ccttcgtgca gccaacatcc tggtgggaga gaacctggtg tgcaaagtgg ccgactttgg
     6541 gctggctcgg ctcattgaag acaatgagta cacggcgcgg caaggtgcca aattccccat
     6601 caagtggacg gctccagaag ctgccctcta tggccgcttc accatcaagt cggacgtgtg
     6661 gtccttcggg atcctgctga ctgagctcac cacaaaggga cgggtgccct accctgggat
     6721 ggtgaaccgc gaggtgctgg accaggtgga gcggggctac cggatgccct gcccgccgga
     6781 gtgtcccgag tccctgcacg acctcatgtg ccagtgctgg cggaaggagc ctgaggagcg
     6841 gcccaccttc gagtacctgc aggccttcct ggaggactac ttcacgtcca ccgagcccta
     6901 g
//
