LOCUS       EF1a-FLEX-Gephyrin.FR-G 7988 bp ds-DNA     circular     22-MAY-2022
DEFINITION  .
KEYWORDS    "marker:AAV2 ITR, AmpR, AAV2 ITR" "ref:pmid\:32674057"
COMMENT     Labeling of excitatory synapses (green fluorescence) in
            cre-expressing neurons. <small>[1] A Viral Toolbox of Genetically
            Encoded Fluorescent Synaptic Tags. iScience. 2020 Jun
            30;23(7):101330. doi: 10.1016/j.isci.2020.101330. Bensussen S,
            Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff SN, Gritton
            HJ, Fabris P, Vanbenschoten H, Beck C, Man HY, Han X
            (pmid:32674057)</small>
FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /label="AAV2 ITR (alternate)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: Functional equivalent of wild-type AAV2 ITR"
     protein_bind    145..156
                     /label="CCR5TC zinc-finger domain ZF binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     protein_bind    145..156
                     /label="ZF binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: CCR5TC zinc-finger domain note: target sequence for the CCR5TC zinc-finger domain from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 2008; Gross et al., 2013)"
     promoter        168..1346
                     /label="EF-1α promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: strong constitutive promoter for human elongation factor EF-1α"
     intron          399..1337
                     /label="EF-1α intron A"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
                     /note="note: intron upstream of the start codon of human EF-1α"
     promoter        1363..1381
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1444..1477
                     /label="lox2272"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Cre recombinase note: Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)."
     protein_bind    1528..1561
                     /label="loxP"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Cre recombinase note: Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)."
     misc_feature    complement(1573..2283)
                     /label="mRuby"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2284..2355)
                     /label="P2A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(2284..2355)
                     /label="Translation 2355-2284"
                     /translation="GVGSGATNFSLLKQAGDVEENPGP"
     misc_feature    complement(2356..2874)
                     /label="CCR5ZF-KRAB"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2911..3621)
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(3658..3948)
                     /label="GPHN-FingR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3952..3960)
                     /label="Kozak"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     protein_bind    complement(3973..4006)
                     /label="lox2272"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Cre recombinase note: Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(4057..4090)
                     /label="loxP"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Cre recombinase note: Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)."
     misc_feature    4115..4703
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: woodchuck hepatitis virus posttranscriptional regulatory element"
     CDS             complement(4586..4597)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="product: Factor Xa recognition and cleavage site codon_start: 1 translation: IEGR transl_table: 1"
     polyA_signal    4735..5211
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: human growth hormone polyadenylation signal"
     repeat_region   5251..5380
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     repeat_region   5251..5391
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="rpt_type: inverted: note: inverted terminal repeat of adeno-associated virus serotype 2"
     rep_origin      5466..5921
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        6203..6307
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             6308..6376
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="product: β-lactamase gene: bla codon_start: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW* note: confers resistance to ampicillin, carbenicillin, and related antibiotics transl_table: 1"
     misc_feature    6308..7168
                     /label="AmpR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             6377..7168
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="product: β-lactamase gene: bla codon_start: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW* note: confers resistance to ampicillin, carbenicillin, and related antibiotics transl_table: 1"
     rep_origin      7339..7927
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcatc ctcatcgaat tccgtgaggc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      421 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1321 tttttcttcc atttcaggtg tcgtgaggaa ttagcttggt actaatacga ctcactatag
     1381 ggagacccaa gctggctagg taagcttggt accgagctcg gatcctctag agtcgactcc
     1441 ggaataactt cgtataggat actttatacg aagttatgca gaatggtagc tggattgtag
     1501 ctgctattag caatatgaaa cctcttaata acttcgtata gcatacatta tacgaagtta
     1561 tggcgcgccT TACTTGTACA GCTCGTCCAT CCCACCACCA AGCCCGGCGA ACTTGGCAAC
     1621 TGCGTGTTCG CGTTGTACTA CGAACATTTC ATTGTCACTT TCCTCTAACC TTTCCAGGCG
     1681 GTGATCAACG GCATGGATAC CGGGCATCTT GATGTTCCCG ACGGTCTTTT TTGACCTGTA
     1741 AGTTGTTACG AAAGAGCAAG ACAGATGGCC ACCACCATCA ACTTTCAGTG CCATATGAGT
     1801 GTATCCCCTC AGACCACCAT CTGCTGGATA CATCATCTCT GTATTAGGCT CCCAACCCTT
     1861 GGTCTTCTTC TGCATCACGG GACCATTGGA GGGAAAGTTT ACCCCTCTGA CTTGGACGTG
     1921 GTAAACGAGA CAGCCATCCT CAAGGCTGGT GTCCTGCATG ACGGTGACGA CTCCACCATC
     1981 TTCGTATCTC GTAACTCTTT CCCAAGTAAA ACCCTCAGGA AAGGACTGTT TAAAGAAATC
     2041 AGGAATGCCT TTCGGGTACT TGATAAAAGT ACGGCTGCCA TACATGAACG ACGTGGCAAG
     2101 AATGTCAAAG GCAAATGGCA GGGGTCCTCC CTCGATGACT TTGATCCTCA TGGTTTGAGT
     2161 TCCCATGTAC GGATTGCCTT CTCCTTCACC TGTGCATTTG AATTGGTGGC CGTTGACCGA
     2221 ACCTTCCATG ACCACCTTCA TACGCATATT TTCCTTGATC AGCTCTTCGC CCTTAGACAC
     2281 CATgggaccg gggttttctt ccacgtctcc tgcttgcttt aacagagaga agttcgtggc
     2341 tccggagccc acaccAGCCA TAGAAGCAAG ATTAGAATAA TTTTCAAGCA TAACTTCACG
     2401 ATAAAGAGAA CGTTGAGAAA GATCAAGTTT TTTCCATTCA TCACGAGTAA AAAGAACAGC
     2461 AACATCTTCA AAAGTAACAG AAACAGAAAC TTGAGAAGCA CCTTCACGTT GTTCAGGAGC
     2521 GTCGACAATC GATAGCTGGC TGCCCCGCAG GTGAATCTTT GTGTGCCGGG TCAGATTGCC
     2581 GGAGGTGGCA AACTTTCTGC CACAGATATC ACAGGCAAAA GGTTTTTCAC CAGTATGTGT
     2641 CCGGATGTGT CTGGCCAGGT TGTCGCTTCT GCTAAAGTTT CTCATACAAA TTCTGCACTG
     2701 GAAAGGTTTC TGGCTGCCAG TGTGGATCTT GGTGTGGCTG TTCAGGTTGC TGCTGATGGC
     2761 GAACTTCCGG CCGCAAATGT CGCAGGCGAA GGGCTTTTCT CCGGTGTGGG TTCTGATGTG
     2821 CCTGCTCAGG TTGGACCGGT CGCTGAAGTT CCGCATGCAG ATCCGGCACT GGAAagtacc
     2881 acctgaacct ccagtaccac ctgaacctcc gtgagcaagg gcgaggagct gttcaccggg
     2941 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3001 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3061 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3121 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3181 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3241 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3301 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3361 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     3421 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3481 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     3541 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3601 ctcggcatgg acgagctgta cagtaccacc tgaacctcca gtaccacctg aacctccCTT
     3661 ATCGTCGTCA TCCTTGTAAT CGGATCCGGT GCGGTAGTTG ATGGAGATCG GGATCGGGTC
     3721 CTGGTAGTTC CAGTGGTCGA TCGTGACGGC GTACACCGTG ATGGTATAGT CGACACCAGG
     3781 TTTCAGGCTG CTGATGGTAG CAGTGGACTT GCTGCCAGGC ACGGTGAATT CCTGGACAGG
     3841 GCTATTGCCA CCAGTTTCAC CGTAGGTGAT GCGGTAGTAG CGAACCATGA CCTTGTACTT
     3901 GCCCCAGCTG ATCTGGATGC TGGTTGGTGA TGCTTCCTTG ACTTCGAGCA TGGTGGCGGC
     3961 ggtggcgcta gcataacttc gtataaagta tcctatacga agttatttgc cttaacccag
     4021 aaattatcac tgttattctt tagaatggtg caaagaataa cttcgtataa tgtatgctat
     4081 acgaagttat gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     4141 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4201 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4261 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4321 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4381 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4441 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4501 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     4561 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4621 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4681 ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg
     4741 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     4801 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     4861 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     4921 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     4981 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     5041 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     5101 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     5161 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg
     5221 taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     5281 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     5341 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     5401 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     5461 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5521 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     5581 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5641 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     5701 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5761 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     5821 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     5881 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5941 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     6001 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     6061 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     6121 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     6181 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6241 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6301 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6361 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6421 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6481 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6541 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6601 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6661 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6721 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6781 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6841 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6901 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6961 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7021 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7081 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7141 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7201 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7261 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7321 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7381 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7441 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7501 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7561 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7621 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7681 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7741 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7801 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7861 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7921 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7981 tcacatgt
//