LOCUS       pMV_AA097              14039 bp ds-DNA     circular SYN 08-APR-2024
DEFINITION  Modular plasmid designed using Fragmid, a plasmid design tool of
            the Genetic Perturbation Platform (GPP), Broad Institute
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Genetic Perturbation Platform (GPP), Broad Institute
COMMENT     Fragmid source plasmids: pRDA_722, pF_AA324, pF_AA065, pF_AA012,
            pF_AA008, pF_AA009, pF_AA014
FEATURES             Location/Qualifiers
     enhancer        238..617
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        619..817
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     LTR             835..1015
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    1062..1187
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Psi"
     misc_feature    1680..1913
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     misc_feature    2440..2557
                     /note="CPPT"
                     /label="CPPT"
     misc_feature    complement(2645..2667)
                     /label="sgCD81_v4 [EnAs]"
     misc_feature    complement(2668..2687)
                     /label="DR_v3 [EnAs]"
     misc_feature    complement(2688..2710)
                     /label="sgCD55_v4 [EnAs]"
     misc_feature    complement(2711..2730)
                     /label="DR_v2 [EnAs]"
     misc_feature    complement(2731..2753)
                     /label="sgCD47_v2 [EnAs]"
     misc_feature    complement(2754..2773)
                     /label="DR_v1 [EnAs]"
     misc_feature    complement(2774..2796)
                     /label="sgCD46_v4 [EnAs]"
     misc_feature    complement(2797..2816)
                     /label="DR_v0 [EnAs]"
     promoter        complement(2819..3067)
                     /label="U6_v1"
     promoter        3141..4319
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /label="EF1a_v1"
     CDS             4330..4332
                     /codon_start=1
                     /transl_table=1
                     /label="Start"
     CDS             4336..4389
                     /codon_start=1
                     /transl_table=1
                     /translation="KRTADGSEFESPKKKRKV"
                     /label="BPNLS_v1.1"
     CDS             4396..8313
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /transl_table=1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYR
                     NAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRS
                     FDKFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLR
                     EHFENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGL
                     NEVLNLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCK
                     YKTLLRNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERR
                     ISELTGKITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALD
                     QPLPTTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEP
                     SLSFYNKARNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGI
                     MPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTP
                     ILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDF
                     LSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLY
                     LFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMA
                     HRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHE
                     IIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLI
                     YITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLS
                     QVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDY
                     PAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIK
                     NHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQF
                     DAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLE
                     NDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDAD
                     ANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN"
                     /label="Cas12a_v1.1 [EnAs]"
     misc_difference 4912..4914
                     /label="E174R"
     misc_difference 6016..6018
                     /label="S542R"
     misc_difference 6034..6036
                     /label="K548R"
     CDS             8314..8319
                     /codon_start=1
                     /transl_table=1
                     /translation="GS"
                     /label="GS"
     CDS             8320..8367
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /transl_table=1
                     /translation="KRPAATKKAGQAKKKK"
                     /label="NpNLS_v1.1"
     CDS             8374..8454
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /transl_table=1
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
                     /label="3xHA_v1.1"
     CDS             8455..8460
                     /codon_start=1
                     /transl_table=1
                     /translation="GS"
                     /label="GS"
     CDS             8461..8517
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A_v1.1"
     CDS             8518..9111
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLE
                     TSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
                     /label="PuroR_v1.1"
     CDS             9112..9117
                     /codon_start=1
                     /transl_table=1
                     /label="GS"
     CDS             9118..9120
                     /codon_start=1
                     /transl_table=1
                     /label="Stop"
     misc_feature    9131..9719
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
     misc_feature    9669
                     /label="Silent"
     LTR             9791..10024
                     /note="self-inactivating 3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (Delta-U3)"
     misc_feature    9820
                     /label="Silent"
     polyA_signal    10056..10280
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     misc_feature    10243
                     /label="Silent"
     rep_origin      10326..10754
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10768..11097
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     rep_origin      10948..11083
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        11145..11192
                     /note="synthetic bacterial promoter"
                     /label="EM7 promoter"
     CDS             11211..11585
                     /codon_start=1
                     /note="BleomycinR"
                     /transl_table=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /label="BleomycinR"
     polyA_signal    11715..11836
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(12287..12875)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13046..13906)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /product="beta-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR"
     promoter        complement(13907..14011)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
      241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
      301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
      361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
      421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
      481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
      541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
      601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
      661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
      721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
      781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT
      841 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT
      901 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC
      961 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC
     1021 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC
     1081 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA
     1141 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG
     1201 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA
     1261 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC
     1321 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA
     1381 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC
     1441 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA
     1501 AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG
     1561 AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA
     1621 GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA
     1681 GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG
     1741 ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG
     1801 CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG
     1861 CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT
     1921 TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT
     1981 AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT
     2041 AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG
     2101 AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA
     2161 ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA
     2221 AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA
     2281 TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA
     2341 GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC GGCACTGCGT
     2401 GCGCCAATTC TGCAGACAAA TGGCAGTATT CATCCACAAT TTTAAAAGAA AAGGGGGGAT
     2461 TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA
     2521 AGAATTACAA AAACAAATTA CAAAAATTCA AAATTTTCGG GTTTATTACA GGGACAGCAG
     2581 AGATCCAGTT TGGTTAATTA AGGTACCGGG CAGAACCGAC TCGGTGCCAC TTTTTCAAAA
     2641 AAAACCTTAC CGTCCCCAGC AGGCACTATC TACTAGAGTA GAAATTTAGT GCCGTCCAGG
     2701 TTACAGAAGA ATCTACGATA GTAGAAATTA GTAGCTGAGC TGATCCTGGA AAGATCTACG
     2761 ACAGTAGAAA TTAGTCTGTG ACAGTAACAG TACTTGATCT ACAAGAGTAG AAATTACCGG
     2821 TGTTTCGTCC TTTCCACAAG ATATATAAAG CCAAGAAATC GAAATACTTT CAAGTTACGG
     2881 TAAGCATATG ATAGTCCATT TTAAAACATA ATTTTAAAAC TGCAAACTAC CCAAGAAATT
     2941 ATTACTTTCT ACGTCACGTA TTTTGTACTA ATATCTTTGT GTTTACAGTC AAATTAATTC
     3001 TAATTATCTC TCTAACAGCC TTGTATCGTA TATGCAAATA TGAAGGAATC ATGGGAAATA
     3061 GGCCCTCTTC GAATCTAGTT GCAAAGATGG ATAAAGTTTT AAACAGAGAG GAATCTTTGC
     3121 AGCTAATGGA CCTTCTACAC GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA
     3181 CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGATC CGGTGCCTAG AGAAGGTGGC
     3241 GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG
     3301 GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG
     3361 CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG
     3421 GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC
     3481 TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC
     3541 GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC
     3601 TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG
     3661 CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG
     3721 GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT
     3781 CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG
     3841 GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA
     3901 GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG
     3961 CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC
     4021 AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG
     4081 CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG
     4141 GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC
     4201 CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT
     4261 TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAA
     4321 GGAGCCACCA TGGCCAAACG GACAGCCGAC GGAAGCGAGT TCGAGTCACC AAAGAAGAAG
     4381 CGGAAAGTCG GCAGCACACA GTTCGAGGGC TTTACCAACC TGTATCAGGT GAGCAAGACA
     4441 CTGCGGTTTG AGCTGATCCC ACAGGGCAAG ACCCTGAAGC ACATCCAGGA GCAGGGCTTC
     4501 ATCGAGGAGG ACAAGGCCCG CAATGATCAC TACAAGGAGC TGAAGCCCAT CATCGATCGG
     4561 ATCTACAAGA CCTATGCCGA CCAGTGCCTG CAGCTGGTGC AGCTGGATTG GGAGAACCTG
     4621 AGCGCCGCCA TCGACTCCTA TAGAAAGGAG AAAACCGAGG AGACAAGGAA CGCCCTGATC
     4681 GAGGAGCAGG CCACATATCG CAATGCCATC CACGACTACT TCATCGGCCG GACAGACAAC
     4741 CTGACCGATG CCATCAATAA GAGACACGCC GAGATCTACA AGGGCCTGTT CAAGGCCGAG
     4801 CTGTTTAATG GCAAGGTGCT GAAGCAGCTG GGCACCGTGA CCACAACCGA GCACGAGAAC
     4861 GCCCTGCTGC GGAGCTTCGA CAAGTTTACA ACCTACTTCT CCGGCTTTTA TCGGAACAGG
     4921 AAGAACGTGT TCAGCGCCGA GGATATCAGC ACAGCCATCC CACACCGCAT CGTGCAGGAC
     4981 AACTTCCCCA AGTTTAAGGA GAATTGTCAC ATCTTCACAC GCCTGATCAC CGCCGTGCCC
     5041 AGCCTGCGGG AGCACTTTGA GAACGTGAAG AAGGCCATCG GCATCTTCGT GAGCACCTCC
     5101 ATCGAGGAGG TGTTTTCCTT CCCTTTTTAT AACCAGCTGC TGACACAGAC CCAGATCGAC
     5161 CTGTATAACC AGCTGCTGGG AGGAATCTCT CGGGAGGCAG GCACCGAGAA GATCAAGGGC
     5221 CTGAACGAGG TGCTGAATCT GGCCATCCAG AAGAATGATG AGACAGCCCA CATCATCGCC
     5281 TCCCTGCCAC ACAGATTCAT CCCCCTGTTT AAGCAGATCC TGTCCGATAG GAACACCCTG
     5341 TCTTTCATCC TGGAGGAGTT TAAGAGCGAC GAGGAAGTGA TCCAGTCCTT CTGCAAGTAC
     5401 AAGACACTGC TGAGAAACGA GAACGTGCTG GAGACAGCCG AGGCCCTGTT TAACGAGCTG
     5461 AACAGCATCG ACCTGACACA CATCTTCATC AGCCACAAGA AGCTGGAGAC AATCAGCAGC
     5521 GCCCTGTGCG ACCACTGGGA TACACTGAGG AATGCCCTGT ATGAGCGGAG AATCTCCGAG
     5581 CTGACAGGCA AGATCACCAA GTCTGCCAAG GAGAAGGTGC AGCGCAGCCT GAAGCACGAG
     5641 GATATCAACC TGCAGGAGAT CATCTCTGCC GCAGGCAAGG AGCTGAGCGA GGCCTTCAAG
     5701 CAGAAAACCA GCGAGATCCT GTCCCACGCA CACGCCGCCC TGGATCAGCC ACTGCCTACA
     5761 ACCCTGAAGA AGCAGGAGGA GAAGGAGATC CTGAAGTCTC AGCTGGACAG CCTGCTGGGC
     5821 CTGTACCACC TGCTGGACTG GTTTGCCGTG GATGAGTCCA ACGAGGTGGA CCCCGAGTTC
     5881 TCTGCCCGGC TGACCGGCAT CAAGCTGGAG ATGGAGCCTT CTCTGAGCTT CTACAACAAG
     5941 GCCAGAAATT ATGCCACCAA GAAGCCCTAC TCCGTGGAGA AGTTCAAGCT GAACTTTCAG
     6001 ATGCCTACAC TGGCCCGCGG CTGGGACGTG AATCGGGAGA AGAACAATGG CGCCATCCTG
     6061 TTTGTGAAGA ACGGCCTGTA CTATCTGGGC ATCATGCCAA AGCAGAAGGG CAGGTATAAG
     6121 GCCCTGAGCT TCGAGCCCAC AGAGAAAACC AGCGAGGGCT TTGATAAGAT GTACTATGAC
     6181 TACTTCCCTG ATGCCGCCAA GATGATCCCA AAGTGCAGCA CCCAGCTGAA GGCCGTGACA
     6241 GCCCACTTTC AGACCCACAC AACCCCCATC CTGCTGTCCA ACAATTTCAT CGAGCCTCTG
     6301 GAGATCACAA AGGAGATCTA CGACCTGAAC AATCCTGAGA AGGAGCCAAA GAAGTTTCAG
     6361 ACAGCCTACG CCAAGAAAAC CGGCGACCAG AAGGGCTACA GAGAGGCCCT GTGCAAGTGG
     6421 ATCGACTTCA CAAGGGATTT TCTGTCCAAG TATACCAAGA CAACCTCTAT CGATCTGTCT
     6481 AGCCTGCGGC CATCCTCTCA GTATAAGGAC CTGGGCGAGT ACTATGCCGA GCTGAATCCC
     6541 CTGCTGTACC ACATCAGCTT CCAGAGAATC GCCGAGAAGG AGATCATGGA TGCCGTGGAG
     6601 ACAGGCAAGC TGTACCTGTT CCAGATCTAT AACAAGGACT TTGCCAAGGG CCACCACGGC
     6661 AAGCCTAATC TGCACACACT GTATTGGACC GGCCTGTTTT CTCCAGAGAA CCTGGCCAAG
     6721 ACAAGCATCA AGCTGAATGG CCAGGCCGAG CTGTTCTACC GCCCTAAGTC CAGGATGAAG
     6781 AGGATGGCAC ACCGGCTGGG AGAGAAGATG CTGAACAAGA AGCTGAAGGA TCAGAAAACC
     6841 CCAATCCCCG ACACCCTGTA CCAGGAGCTG TACGACTATG TGAATCACAG ACTGTCCCAC
     6901 GACCTGTCTG ATGAGGCCAG GGCCCTGCTG CCCAACGTGA TCACCAAGGA GGTGTCTCAC
     6961 GAGATCATCA AGGATAGGCG CTTTACCAGC GACAAGTTCT TTTTCCACGT GCCTATCACA
     7021 CTGAACTATC AGGCCGCCAA TTCCCCATCT AAGTTCAACC AGAGGGTGAA TGCCTACCTG
     7081 AAGGAGCACC CCGAGACACC TATCATCGGC ATCGATCGGG GCGAGAGAAA CCTGATCTAT
     7141 ATCACAGTGA TCGACTCCAC CGGCAAGATC CTGGAGCAGC GGAGCCTGAA CACCATCCAG
     7201 CAGTTTGATT ACCAGAAGAA GCTGGACAAC AGGGAGAAGG AGAGGGTGGC AGCAAGGCAG
     7261 GCCTGGTCTG TGGTGGGCAC AATCAAGGAT CTGAAGCAGG GCTATCTGAG CCAGGTCATC
     7321 CACGAGATCG TGGACCTGAT GATCCACTAC CAGGCCGTGG TGGTGCTGGA GAACCTGAAT
     7381 TTCGGCTTTA AGAGCAAGAG GACCGGCATC GCCGAGAAGG CCGTGTACCA GCAGTTCGAG
     7441 AAGATGCTGA TCGATAAGCT GAATTGCCTG GTGCTGAAGG ACTATCCAGC AGAGAAAGTG
     7501 GGAGGCGTGC TGAACCCATA CCAGCTGACA GACCAGTTCA CCTCCTTTGC CAAGATGGGC
     7561 ACCCAGTCTG GCTTCCTGTT TTACGTGCCT GCCCCATATA CATCTAAGAT CGATCCCCTG
     7621 ACCGGCTTCG TGGACCCCTT CGTGTGGAAA ACCATCAAGA ATCACGAGAG CCGCAAGCAC
     7681 TTCCTGGAGG GCTTCGACTT TCTGCACTAC GACGTGAAAA CCGGCGACTT CATCCTGCAC
     7741 TTTAAGATGA ACAGAAATCT GTCCTTCCAG AGGGGCCTGC CCGGCTTTAT GCCTGCATGG
     7801 GATATCGTGT TCGAGAAGAA CGAGACACAG TTTGACGCCA AGGGCACCCC TTTCATCGCC
     7861 GGCAAGAGAA TCGTGCCAGT GATCGAGAAT CACAGATTCA CCGGCAGATA CCGGGACCTG
     7921 TATCCTGCCA ACGAGCTGAT CGCCCTGCTG GAGGAGAAGG GCATCGTGTT CAGGGATGGC
     7981 TCCAACATCC TGCCAAAGCT GCTGGAGAAT GACGATTCTC ACGCCATCGA CACCATGGTG
     8041 GCCCTGATCC GCAGCGTGCT GCAGATGCGG AACTCCAATG CCGCCACAGG CGAGGACTAT
     8101 ATCAACAGCC CCGTGCGCGA TCTGAATGGC GTGTGCTTCG ACTCCCGGTT TCAGAACCCA
     8161 GAGTGGCCCA TGGACGCCGA TGCCAATGGC GCCTACCACA TCGCCCTGAA GGGCCAGCTG
     8221 CTGCTGAATC ACCTGAAGGA GAGCAAGGAT CTGAAGCTGC AGAACGGCAT CTCCAATCAG
     8281 GACTGGCTGG CCTACATCCA GGAGCTGCGC AACGGGTCCA AAAGGCCGGC GGCCACGAAA
     8341 AAGGCCGGCC AGGCAAAAAA GAAAAAGGGA TCCTACCCAT ACGATGTTCC AGATTACGCT
     8401 TATCCCTACG ACGTGCCTGA TTATGCATAC CCATATGATG TCCCCGACTA TGCCGGAAGC
     8461 GCAACAAACT TCTCTCTGCT GAAACAAGCC GGAGATGTCG AAGAGAATCC TGGACCGACC
     8521 GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC
     8581 CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC
     8641 ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC
     8701 AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC
     8761 GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG
     8821 CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG
     8881 TGGTTCCTGG CCACCGTCGG AGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC
     8941 GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC
     9001 TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC
     9061 GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CGGATCCTGA
     9121 TAAATTCGAA AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA
     9181 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT
     9241 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA
     9301 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC
     9361 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT
     9421 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG
     9481 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC
     9541 TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC
     9601 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT
     9661 TCCGCGTCCT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCG
     9721 TCGACTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA
     9781 AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAT AAGATCTGCT TTTTGCTTGT
     9841 ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC
     9901 CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG
     9961 TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT
    10021 AGCAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC
    10081 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT
    10141 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG
    10201 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GATAATAGCA GGCATGCTGG
    10261 GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA
    10321 TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT
    10381 GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT
    10441 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG
    10501 ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG
    10561 TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
    10621 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA
    10681 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA
    10741 ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC
    10801 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA
    10861 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA
    10921 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT
    10981 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC
    11041 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
    11101 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT
    11161 CGGCATAGTA TATCGGCATA GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT
    11221 TGACCAGTGC CGTTCCGGTG CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA
    11281 CCGACCGGCT CGGGTTCTCC CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG
    11341 ACGACGTGAC CCTGTTCATC AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG
    11401 CCTGGGTGTG GGTGCGCGGC CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA
    11461 CGAACTTCCG GGACGCCTCC GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC
    11521 GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG
    11581 ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
    11641 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT
    11701 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA
    11761 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC
    11821 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT
    11881 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC
    11941 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA
    12001 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
    12061 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC
    12121 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG
    12181 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG
    12241 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
    12301 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
    12361 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
    12421 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
    12481 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
    12541 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
    12601 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
    12661 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
    12721 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
    12781 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
    12841 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
    12901 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
    12961 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
    13021 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
    13081 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
    13141 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC
    13201 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC
    13261 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA
    13321 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC
    13381 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT
    13441 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA
    13501 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
    13561 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG
    13621 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC
    13681 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT
    13741 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT
    13801 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
    13861 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC
    13921 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA
    13981 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAC
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