LOCUS       Exported                5306 bp ds-DNA     circular SYN 06-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 8, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        16..1194
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          247..1185
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      1218..1227
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1227..1256
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1302..1961
                     /codon_start=1
                     /label=Rab3A
                     /translation="MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADD
                     SFTPAFVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDI
                     TNEESFNAVQDWSTQIKTYSWDNAQVLLVGNKCDMEDERVVSSERGRQLADHLGFEFFE
                     ASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQGPQLTDQQAPPHQDCAC"
     polyA_signal    2094..2215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2222..2677)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2704..2808
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2810..3167
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3018..3153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3202..3996
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4228..4275
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4604..5192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcggccgcct cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
       61 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
      121 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      181 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      241 acacaggtaa gtgccgtgtg tggttccggc gggcctggcc tctttacggg ttatggccct
      301 tgcgtgcctt gaattacttc cacctggcag cagtacgtga ttcttgatcc cgagcttcgg
      361 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
      421 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
      481 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
      541 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatcagca cactggtatt
      601 tcggtttttg ggggcgcggg cggcgacggg ggccgtgcgt cccagcgcac atgttcggcg
      661 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
      721 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
      781 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctggaggg
      841 tgctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
      901 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
      961 tccaggcacc tcgattagtt gtcgagcttt tggagtacgt cgtctttagg ttggggggag
     1021 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1081 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1141 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt
     1201 ggctagcgct accggtcgcc accatggaac aaaaacttat ttcagaagaa gatttgtccg
     1261 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga catggcctca gccacagact
     1321 ctcgatatgg gcagaaggag tcctcagacc agaacttcga ctatatgttc aagatcctga
     1381 tcattggtaa cagcagtgtg ggcaaaacct cattcctttt ccgctacgca gacgactctt
     1441 ttactccagc ctttgtcagc actgtgggca tagacttcaa ggtcaaaacc atctaccgca
     1501 atgacaagag gatcaagctg cagatctggg acacagcagg acaagagcgg taccgaacca
     1561 tcaccacagc ctactaccgg ggcgccatgg gcttcattct aatgtatgac atcaccaatg
     1621 aggagtcctt caatgcagtg caggactggt ccactcagat caaaacttac tcatgggaca
     1681 atgcccaggt gctgctggtg gggaacaagt gcgacatgga ggacgagcga gtggtgtcct
     1741 cagaacgagg ccggcagctg gccgaccacc tgggctttga gttctttgag gccagcgcca
     1801 aggacaacat taatgtcaag cagacctttg aacgtctggt ggacgtgatc tgtgagaaga
     1861 tgtcggagtc cctagatact gcagaccctg cagtcacggg tgccaagcag ggcccacagc
     1921 tcaccgacca gcaggcacca cctcatcagg attgcgcctg ctgaggatcc accggatcta
     1981 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2041 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2101 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2161 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     2221 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2281 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2341 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2401 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     2461 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     2521 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     2581 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     2641 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     2701 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2761 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     2821 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     2881 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     2941 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3001 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3061 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3121 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     3181 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3241 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3301 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     3361 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     3421 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     3481 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     3541 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     3601 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     3661 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     3721 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     3781 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     3841 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     3901 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3961 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4021 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4081 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4141 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     4201 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     4261 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     4321 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     4381 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     4441 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4501 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4561 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4621 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4681 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4741 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4801 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4861 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4921 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4981 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5041 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5101 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5161 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5221 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5281 tggataaccg tattaccgcc atgcat
//
