LOCUS       pICSL30002              2522 bp ds-DNA     circular     05-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   8..11
                     /label="CCAT 4bp overhang"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             10..283
                     /label="C terminus of YFP (Aequorea victoria)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTSRRTASRPTSRSATTSRTAACSSPTTTSRTPPSATAPCCCPTTTT*ATSPP*AKTPTRSAITWSCWSS*PPPGSLSAWTSCTRVTRLLD"
     Misc._feature   284..287
                     /label="AATG 4bp overhang"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(302..351)
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
     rep_origin      complement(534..1122)
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1296..2084)
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(2091..2147)
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             2235..2315
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     promoter        complement(2306..2410)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ggtctcacca tGACAAGCAG AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGCCACAACA
       61 TCGAGGACGG CGGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG
      121 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG AGCAAAGACC
      181 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC
      241 TCGGCATGGA CGAGCTGTAC AAGGGTAACG CGGCTATTAG ATCaatgtga gaccacgagt
      301 gattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      361 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      421 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      481 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      541 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      601 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      661 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      721 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      781 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      841 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      901 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      961 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1021 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1081 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1141 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1201 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1261 taaagtatat atgagtaaac ttggtctgac agttatttgc cgactacctt ggtgatctcg
     1321 ccttttacgt aatggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     1381 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
     1441 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
     1501 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
     1561 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
     1621 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     1681 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
     1741 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     1801 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
     1861 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     1921 gtcaccgtaa ccagcaaatc aatatcgctg tgtggcttca ggccgccatc cactgcggag
     1981 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
     2041 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt taactttgtt
     2101 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     2161 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaaaac
     2221 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     2281 ttctggacca gttgcgtgag cgcatactct tcctttttca atattattga agcatttatc
     2341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2401 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2461 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtccactg aagagccact
     2521 gt
//