LOCUS       pICSL30014      2772 bp ds-DNA     circular SYN 10-MAR-2016
DEFINITION  High copy BioBrick assembly plasmid with a chloramphenicol 
            resistance marker, for shipping parts to the Registry of Standard 
            Biological Parts.
ACCESSION   .
VERSION     .
KEYWORDS    pICSL30014
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   iGEM
  TITLE     Direct Submission
  JOURNAL   Exported 10 Mar 2016 from SnapGene Viewer 2.8.1
            http://www.snapgene.com
COMMENT     VNTDATE|687830400|
FEATURES             Location/Qualifiers
     Misc._feature   complement(259..847)
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ff4062; direction: LEFT"
     Terminator      1030..1124
                     /label="lambda t0 terminator"
     CDS             complement(1145..1804)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="cat"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #800000"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     CDS             2049..2762
                     /label=tagBFP2
                     /note="tagBFP2"
ORIGIN
        1 CTGCAGTCCG GCAAAAAAGG GCAAGGTGTC ACCACCCTGC CCTTTTTCTT TAAAACCGAA
       61 AAGATTACTT CGCGTTATGC AGGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG
      121 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG
      181 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
      241 GGCCGCGTTG CTGGCGTTTT TCCACAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
      301 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
      361 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
      421 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
      481 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
      541 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
      601 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
      661 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
      721 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
      781 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
      841 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
      901 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
      961 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGCTC
     1021 GAGGCTTGGA TTCTCACCAA TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC
     1081 CAGATGGAGT TCTGAGGTCA TTACTGGATC TATCAACAGG AGTCCAAGCG AGCTCGATAT
     1141 CAAATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC
     1201 CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT
     1261 TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT
     1321 TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA
     1381 TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT
     1441 GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA
     1501 ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA
     1561 GCTCACCGTC TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA
     1621 TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG
     1681 TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA
     1741 AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT
     1801 CCATTTTAGC TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG
     1861 ATCTTATTTC ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCCGATCAAC TCGAGTGCCA
     1921 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
     1981 AGGCAGAATT TCAGATAAAA AAAATCCTTA GCTTTCGCTA AGGATGATTT CTGGAATTCG
     2041 GTCTCACCAT GGTGTCTAAG GGCGAAGAGC TGATTAAGGA GAACATGCAC ATGAAGCTGT
     2101 ACATGGAGGG CACCGTGGAC AACCATCACT TCAAGTGCAC ATCCGAGGGC GAAGGCAAGC
     2161 CCTACGAGGG CACCCAGACC ATGAGAATCA AGGTGGTCGA GGGCGGCCCT CTCCCCTTCG
     2221 CCTTCGACAT CCTGGCTACT AGCTTCCTCT ACGGCAGCAA GACCTTCATC AACCACACCC
     2281 AGGGCATCCC CGACTTCTTC AAGCAGTCCT TCCCTGAGGG CTTCACATGG GAGAGAGTCA
     2341 CCACATACGA GGACGGGGGC GTGCTGACCG CTACCCAGGA CACCAGCCTC CAGGACGGCT
     2401 GCCTCATCTA CAACGTCAAG ATCAGAGGGG TGAACTTCAC ATCCAACGGC CCTGTGATGC
     2461 AGAAGAAAAC ACTCGGCTGG GAGGCCTTCA CCGAGACGCT GTACCCCGCT GACGGCGGCC
     2521 TGGAAGGCAG AAACGACATG GCCCTGAAGC TCGTGGGCGG GAGCCATCTG ATCGCAAACG
     2581 CCAAGACCAC ATATAGATCC AAGAAACCCG CTAAGAACCT CAAGATGCCT GGCGTCTACT
     2641 ATGTGGACTA CAGACTGGAA AGAATCAAGG AGGCCAACAA CGAAACCTAC GTCGAGCAGC
     2701 ACGAGGTGGC AGTGGCCAGA TACTGCGACC TCCCTAGCAA ACTGGGGCAC AAGCTTAATT
     2761 CAATGCGAGA CC
//
