LOCUS       pICSL30021              2586 bp ds-DNA     circular     05-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..351
                     /label="C3_Cysta"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             complement(366..415)
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
     rep_origin      complement(598..1186)
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1360..2148)
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(2155..2211)
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             2299..2379
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     promoter        complement(2370..2474)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ggtctcacca tGCCGAATCC TGGGGGCATT ACCAATGTTC CATTCCCAAA CCTCCCCCAG
       61 TTCAAAGATC TTGCTCGTTT TGCTGTTCAA GATTATAATA AGAAAGAGAA TGCTCATTTG
      121 GAGTTTGTAG AAAATTTGAA TGTGAAGGAA CAAGTTGTTG CTGGAATAAT ATACTATATA
      181 ACGCTTGTGG CAACTGATGC TCATCACCAT CACCATCACG GAAAGAAGAA AATATATGAA
      241 ACCAAGATTT TGGTGAAGGG ATGGGAGAAT TTCAAGGAAG TTCAAGAGTT CAAGCTTGTT
      301 GGTGATGCCA CTAAGAGCAG CGGTCTGGAA GTTCTGTTTC AGGGCCCaat gtgagaccac
      361 gagtgattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      421 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      481 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      541 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      601 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      721 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      781 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      841 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      901 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      961 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1021 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1081 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1141 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1201 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1261 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1321 aatctaaagt atatatgagt aaacttggtc tgacagttat ttgccgacta ccttggtgat
     1381 ctcgcctttt acgtaatgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1441 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1501 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1561 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1621 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1681 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1741 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1801 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1861 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1921 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1981 tacggtcacc gtaaccagca aatcaatatc gctgtgtggc ttcaggccgc catccactgc
     2041 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2101 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatga tgtttaactt
     2161 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2281 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     2341 aaggttctgg accagttgcg tgagcgcata ctcttccttt ttcaatatta ttgaagcatt
     2401 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2461 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2521 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtcc actgaagagc
     2581 cactgt
//