LOCUS       pICSL30024              2310 bp ds-DNA     circular     05-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..75
                     /label="10H-Strep"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             complement(90..139)
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
     rep_origin      complement(322..910)
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1084..1872)
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(1879..1935)
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             2023..2103
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     promoter        complement(2094..2198)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ggtctcacca tGCATCACCA TCACCATCAC CATCACCATC ACTCATGGAG CCACCCGCAG
       61 TTCGAAAAAT Caatgtgaga ccacgagtga ttaatgaatc ggccaacgcg cggggagagg
      121 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      181 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      241 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      301 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      361 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      421 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      481 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      541 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      601 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      661 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      721 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      781 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      841 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      901 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      961 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1021 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1081 ttatttgccg actaccttgg tgatctcgcc ttttacgtaa tggacaaatt cttccaactg
     1141 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     1201 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     1261 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     1321 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     1381 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     1441 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     1501 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     1561 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     1621 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     1681 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcgctgtg
     1741 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     1801 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     1861 cgcttccctc atgatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     1921 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1981 aggcatagac tgtaccccaa aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catactcttc
     2101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2221 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2281 aggccctttc gtccactgaa gagccactgt
//