LOCUS       pICSL30034              3384 bp ds-DNA     circular     05-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    10..93
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="3xFLAG"
                     /label="3xFLAG"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    94..129
                     /label="GGGS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /locus_tag="GGGS"
                     /label="GGGS"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    130..1110
                     /label="TurboID"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="TurboID"
                     /label="TurboID"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1111..1146
                     /label="GGGS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /locus_tag="GGGS(1)"
                     /label="GGGS(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             complement(1164..1213)
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
     rep_origin      complement(1396..1984)
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(2158..2946)
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(2953..3009)
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             3097..3177
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     promoter        complement(3168..3272)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ggtctcacca tGGATTATAA GGACCATGAC GGAGACTATA AGGACCATGA CCTCGACGCT
       61 GCAGCAGCTG ATTATAAGGA CGATGACGAT AAAGGCGGAG GTTCAGGGGG TGGCTCAGGT
      121 GGCGGATCAA TGAAAGACAA TACTGTGCCT CTGAAGCTGA TCGCTCTCCT GGCTAATGGC
      181 GAGTTCCATA GTGGCGAACA GCTGGGAGAA ACCCTGGGCA TGTCCAGGGC CGCTATCAAC
      241 AAGCACATTC AGACTCTGCG CGACTGGGGC GTGGACGTGT TCACCGTGCC CGGAAAGGGC
      301 TACTCTCTGC CCGAGCCTAT CCCGCTGCTG AACGCTAAAC AGATTTTGGG ACAGCTGGAC
      361 GGCGGGAGCG TGGCAGTCCT GCCTGTGGTC GACTCCACCA ATCAGTACCT GCTGGATCGA
      421 ATCGGCGAGC TGAAGAGTGG GGATGCTTGC ATTGCAGAAT ATCAGCAGGC AGGGAGAGGA
      481 AGCAGAGGGA GGAAATGGTT CTCTCCTTTT GGAGCTAACC TGTACCTGAG TATGTTTTGG
      541 CGCCTGAAGC GGGGACCAGC AGCAATCGGC CTGGGCCCGG TCATCGGAAT TGTCATGGCA
      601 GAAGCGCTGC GAAAGCTGGG AGCAGACAAG GTGCGAGTCA AATGGCCCAA TGACCTGTAT
      661 CTGCAGGATA GAAAGCTGGC AGGCATCCTG GTGGAGCTGG CCGGAATAAC AGGCGATGCT
      721 GCACAGATCG TCATTGGCGC CGGGATTAAC GTGGCTATGA GGCGCGTGGA GGAAAGCGTG
      781 GTCAATCAGG GCTGGATCAC ACTGCAGGAA GCAGGGATTA ACCTGGACAG GAATACTCTG
      841 GCCGCTACGC TGATCCGAGA GCTGCGGGCA GCCCTGGAAC TGTTCGAGCA GGAAGGCCTG
      901 GCTCCATATC TGCCACGGTG GGAGAAGCTG GATAACTTCA TCAATAGACC CGTGAAGCTG
      961 ATCATTGGGG ACAAAGAGAT TTTCGGGATT AGCCGGGGGA TTGATAAACA GGGAGCCCTG
     1021 CTGCTGGAAC AGGACGGAGT TATCAAACCC TGGATGGGCG GAGAAATCAG TCTGCGGTCT
     1081 GCCGAAAAGA AGCTTGCGGC CGCACTCGAG GGAGGTGGAT CTGGTGGAGG ATCTGGAGGA
     1141 GGTTCaatgt gagaccacga gtgattaatg aatcggccaa cgcgcgggga gaggcggttt
     1201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1561 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1801 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttattt
     2161 gccgactacc ttggtgatct cgccttttac gtaatggaca aattcttcca actgatctgc
     2221 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac
     2281 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc
     2341 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc
     2401 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa
     2461 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac
     2521 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc
     2581 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc
     2641 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag
     2701 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg
     2761 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcgc tgtgtggctt
     2821 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg
     2881 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc
     2941 cctcatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
     3001 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     3061 agactgtacc ccaaaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     3121 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatact cttccttttt
     3181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3301 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3361 tttcgtccac tgaagagcca ctgt
//