LOCUS       pICSL30043              2292 bp ds-DNA     circular     05-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..57
                     /label="NTAG NLS (MASSPPKKKRKVSWKM)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="NTAG NLS (MASSPPKKKRKVSWKM)"
                     /label="NTAG NLS (MASSPPKKKRKVSWKM)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     CDS             complement(72..121)
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
     rep_origin      complement(304..892)
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1066..1854)
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(1861..1917)
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             2005..2085
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     promoter        complement(2076..2180)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ggtctcacca tGGCTTCTTC TCCACCTAAG AAGAAGAGAA AGGTTTCTTG GAAaatgtga
       61 gaccacgagt gattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      121 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      181 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      241 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      481 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      661 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      721 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      781 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      841 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      901 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      961 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1021 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttatttgc cgactacctt
     1081 ggtgatctcg ccttttacgt aatggacaaa ttcttccaac tgatctgcgc gcgaggccaa
     1141 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
     1201 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
     1261 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
     1321 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
     1381 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
     1441 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
     1501 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
     1561 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
     1621 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
     1681 aagccttacg gtcaccgtaa ccagcaaatc aatatcgctg tgtggcttca ggccgccatc
     1741 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
     1801 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt
     1861 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1921 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1981 aaaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     2041 tcggtcaagg ttctggacca gttgcgtgag cgcatactct tcctttttca atattattga
     2101 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2161 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2221 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtccactg
     2281 aagagccact gt
//