LOCUS       pICSL30063              2526 bp ds-DNA     circular     05-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..291
                     /label="Protein Select Hellfire"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /locus_tag="Protein Select Hellfire"
                     /label="Protein Select Hellfire"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     CDS             complement(306..355)
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
     rep_origin      complement(538..1126)
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1300..2088)
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(2095..2151)
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             2239..2319
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     promoter        complement(2310..2414)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ggtctcacca tGGCAAAGTA TCATCACCAT CACCATCACG ATTATGATAT TCCAACTACT
       61 GCTAGCGAGA ATTTGTATTT TCAGGGTGAG CTCGACTACA AAGACCATGA CGGTGATTAT
      121 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGA ACGCGGCTAT TAGATCAGGA
      181 GTGAAAATTG TGAGCCGCAA AAGCCTGGGC GTGCAGAATG TGTATGATAT TGGCGTGGAA
      241 AAAGATCATA ACTTTCTGCT GGCGAATGGC CTGATTGCGA GCAACGGaat gtgagaccac
      301 gagtgattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      361 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      421 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      481 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      541 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      601 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      661 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      721 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      781 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      841 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      901 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      961 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1021 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1081 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1141 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1201 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1261 aatctaaagt atatatgagt aaacttggtc tgacagttat ttgccgacta ccttggtgat
     1321 ctcgcctttt acgtaatgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1381 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1441 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1501 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1561 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1621 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1681 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1741 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1801 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1861 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1921 tacggtcacc gtaaccagca aatcaatatc gctgtgtggc ttcaggccgc catccactgc
     1981 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     2041 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatga tgtttaactt
     2101 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     2161 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2221 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     2281 aaggttctgg accagttgcg tgagcgcata ctcttccttt ttcaatatta ttgaagcatt
     2341 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2401 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2461 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtcc actgaagagc
     2521 cactgt
//