LOCUS       pICSL30071              3468 bp ds-DNA     circular     05-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..22
                     /label="MBP"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_fwdcolor="#00ffff"
                     /locus_tag="MBP"
                     /label="MBP"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    23..42
                     /label="6HIS_C3"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_fwdcolor="#00ffff"
                     /locus_tag="6HIS_C3"
                     /label="6HIS_C3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    43..87
                     /label="AviTAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="AviTAG"
                     /label="AviTAG"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    88..1233
                     /label="MBP"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_fwdcolor="#00ffff"
                     /locus_tag="MBP(1)"
                     /label="MBP(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             complement(1248..1297)
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
     rep_origin      complement(1480..2068)
                     /label="ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(2242..3030)
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(3037..3093)
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             3181..3261
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     promoter        complement(3252..3356)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ggtctcacca tGGCACACCA TCACCACCAT CACAGCAGCG GTGGACTGAA TGATATATTC
       61 GAGGCTCAGA AGATTGAATA CCACGAGAGC AGCGGCATGA AAATCGAAGA AGGTAAACTG
      121 GTAATCTGGA TTAACGGCGA TAAAGGCTAT AACGGCCTCG CTGAAGTCGG TAAGAAATTC
      181 GAGAAAGATA CCGGAATTAA AGTCACCGTT GAGCATCCGG ATAAACTGGA AGAGAAATTC
      241 CCACAGGTTG CGGCAACTGG CGATGGCCCT GACATTATCT TCTGGGCACA CGACCGCTTT
      301 GGTGGCTACG CTCAATCTGG CCTGTTGGCT GAAATCACCC CGGACAAAGC GTTCCAGGAC
      361 AAGCTGTATC CGTTTACCTG GGATGCCGTA CGTTACAACG GCAAGCTGAT TGCTTACCCG
      421 ATCGCTGTTG AAGCGTTATC GCTGATTTAT AACAAAGATC TGCTGCCGAA CCCGCCAAAA
      481 ACCTGGGAAG AGATCCCGGC GCTGGATAAA GAACTGAAAG CGAAAGGTAA GAGCGCGCTG
      541 ATGTTCAACC TGCAAGAACC GTACTTCACC TGGCCGCTGA TTGCTGCTGA CGGGGGTTAT
      601 GCGTTCAAGT ATGAAAACGG CAAGTACGAC ATTAAAGACG TGGGCGTGGA TAACGCTGGC
      661 GCGAAAGCGG GTCTGACCTT CCTGGTTGAC CTGATTAAAA ACAAACACAT GAATGCAGAC
      721 ACCGATTACT CCATCGCAGA AGCTGCCTTT AATAAAGGCG AAACAGCGAT GACCATCAAC
      781 GGCCCGTGGG CATGGTCCAA CATCGACACC AGCAAAGTGA ATTATGGTGT AACGGTACTG
      841 CCGACCTTCA AGGGTCAACC ATCCAAACCG TTCGTTGGCG TGCTGAGCGC AGGTATTAAC
      901 GCCGCCAGTC CGAACAAAGA GCTGGCAAAA GAGTTCCTCG AAAACTATCT GCTGACTGAT
      961 GAAGGTCTGG AAGCGGTTAA TAAAGACAAA CCGCTGGGTG CCGTAGCGCT GAAGTCTTAC
     1021 GAGGAAGAGT TGGCGAAAGA TCCACGTATT GCCGCCACTA TGGAAAACGC CCAGAAAGGT
     1081 GAAATCATGC CGAACATCCC GCAGATGTCC GCTTTCTGGT ATGCCGTGCG TACTGCGGTG
     1141 ATCAACGCCG CCAGCGGTCG TCAGACTGTC GATGAAGCCC TGAAAGACGC GCAGACTAGC
     1201 AGCGGTCTGG AAGTTCTGTT TCAGGGCCCa atgtgagacc acgagtgatt aatgaatcgg
     1261 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1321 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1381 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1921 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2221 gtaaacttgg tctgacagtt atttgccgac taccttggtg atctcgcctt ttacgtaatg
     2281 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
     2341 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     2401 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
     2461 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
     2521 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
     2581 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
     2641 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     2701 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     2761 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
     2821 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
     2881 caaatcaata tcgctgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
     2941 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
     3001 cgatacttcg gcgatcaccg cttccctcat gatgtttaac tttgttttag ggcgactgcc
     3061 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     3121 ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaaaacagtc ataacaagcc
     3181 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
     3241 cgtgagcgca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3361 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3421 aaaaataggc gtatcacgag gccctttcgt ccactgaaga gccactgt
//