LOCUS       pICSL50035              3280 bp ds-DNA     circular     06-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..1047
                     /label="TurboID"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        1166..1270
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1261..1341)
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             1429..1485
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             1492..2280
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2454..3042
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3225..3274
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
ORIGIN
        1 ggtctcattc gATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGCGGA TCCGAATTCA
       61 TGAAAGACAA TACTGTGCCT CTGAAGCTGA TCGCTCTCCT GGCTAATGGC GAGTTCCATA
      121 GTGGCGAACA GCTGGGAGAA ACCCTGGGCA TGTCCAGGGC CGCTATCAAC AAGCACATTC
      181 AGACTCTGCG CGACTGGGGC GTGGACGTGT TCACCGTGCC CGGAAAGGGC TACTCTCTGC
      241 CCGAGCCTAT CCCGCTGCTG AACGCTAAAC AGATTTTGGG ACAGCTGGAC GGCGGGAGCG
      301 TGGCAGTCCT GCCTGTGGTC GACTCCACCA ATCAGTACCT GCTGGATCGA ATCGGCGAGC
      361 TGAAGAGTGG GGATGCTTGC ATTGCAGAAT ATCAGCAGGC AGGGAGAGGA AGCAGAGGGA
      421 GGAAATGGTT CTCTCCTTTT GGAGCTAACC TGTACCTGAG TATGTTTTGG CGCCTGAAGC
      481 GGGGACCAGC AGCAATCGGC CTGGGCCCGG TCATCGGAAT TGTCATGGCA GAAGCGCTGC
      541 GAAAGCTGGG AGCAGACAAG GTGCGAGTCA AATGGCCCAA TGACCTGTAT CTGCAGGATA
      601 GAAAGCTGGC AGGCATCCTG GTGGAGCTGG CCGGAATAAC AGGCGATGCT GCACAGATCG
      661 TCATTGGCGC CGGGATTAAC GTGGCTATGA GGCGCGTGGA GGAAAGCGTG GTCAATCAGG
      721 GCTGGATCAC ACTGCAGGAA GCAGGGATTA ACCTGGACAG GAATACTCTG GCCGCTACGC
      781 TGATCCGAGA GCTGCGGGCA GCCCTGGAAC TGTTCGAGCA GGAAGGCCTG GCTCCATATC
      841 TGCCACGGTG GGAGAAGCTG GATAACTTCA TCAATAGACC CGTGAAGCTG ATCATTGGGG
      901 ACAAAGAGAT TTTCGGGATT AGCCGGGGGA TTGATAAACA GGGAGCCCTG CTGCTGGAAC
      961 AGGACGGAGT TATCAAACCC TGGATGGGCG GAGAAATCAG TCTGCGGTCT GCCGAAAAGA
     1021 AGCTTGCGGC CGCACTCGAG TGAgctttga gaccacgtgg ctcttcagtg gacgaaaggg
     1081 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1141 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1201 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1261 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1321 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
     1381 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     1441 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     1501 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     1561 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     1621 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     1681 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     1741 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     1801 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     1861 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     1921 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     1981 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     2041 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     2101 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     2161 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     2221 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
     2281 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2581 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3121 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3181 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3241 ccgcctctcc ccgcgcgttg gccgattcat taatcactgt
//