LOCUS       pICSL50040              3361 bp ds-DNA     circular     06-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..1128
                     /label="TurboID+3xFLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        1247..1351
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1342..1422)
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             1510..1566
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             1573..2361
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2535..3123
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3306..3355
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
ORIGIN
        1 ggtctcattc gATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGCGGA TCCGAATTCA
       61 TGAAAGACAA TACTGTGCCT CTGAAGCTGA TCGCTCTCCT GGCTAATGGC GAGTTCCATA
      121 GTGGCGAACA GCTGGGAGAA ACCCTGGGCA TGTCCAGGGC CGCTATCAAC AAGCACATTC
      181 AGACTCTGCG CGACTGGGGC GTGGACGTGT TCACCGTGCC CGGAAAGGGC TACTCTCTGC
      241 CCGAGCCTAT CCCGCTGCTG AACGCTAAAC AGATTTTGGG ACAGCTGGAC GGCGGGAGCG
      301 TGGCAGTCCT GCCTGTGGTC GACTCCACCA ATCAGTACCT GCTGGATCGA ATCGGCGAGC
      361 TGAAGAGTGG GGATGCTTGC ATTGCAGAAT ATCAGCAGGC AGGGAGAGGA AGCAGAGGGA
      421 GGAAATGGTT CTCTCCTTTT GGAGCTAACC TGTACCTGAG TATGTTTTGG CGCCTGAAGC
      481 GGGGACCAGC AGCAATCGGC CTGGGCCCGG TCATCGGAAT TGTCATGGCA GAAGCGCTGC
      541 GAAAGCTGGG AGCAGACAAG GTGCGAGTCA AATGGCCCAA TGACCTGTAT CTGCAGGATA
      601 GAAAGCTGGC AGGCATCCTG GTGGAGCTGG CCGGAATAAC AGGCGATGCT GCACAGATCG
      661 TCATTGGCGC CGGGATTAAC GTGGCTATGA GGCGCGTGGA GGAAAGCGTG GTCAATCAGG
      721 GCTGGATCAC ACTGCAGGAA GCAGGGATTA ACCTGGACAG GAATACTCTG GCCGCTACGC
      781 TGATCCGAGA GCTGCGGGCA GCCCTGGAAC TGTTCGAGCA GGAAGGCCTG GCTCCATATC
      841 TGCCACGGTG GGAGAAGCTG GATAACTTCA TCAATAGACC CGTGAAGCTG ATCATTGGGG
      901 ACAAAGAGAT TTTCGGGATT AGCCGGGGGA TTGATAAACA GGGAGCCCTG CTGCTGGAAC
      961 AGGACGGAGT TATCAAACCC TGGATGGGCG GAGAAATCAG TCTGCGGTCT GCCGAAAAGA
     1021 AGCTTGCGGC CGCACTCGAG GATTATAAGG ACCATGACGG AGACTATAAG GACCATGACC
     1081 TCGACGCTGC AGCAGCTGAT TATAAGGACG ATGACGATAA GTGAgctttg agaccacgtg
     1141 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1201 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1261 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1321 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
     1381 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1441 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1501 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1561 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1621 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1681 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     1741 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1801 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1861 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1921 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1981 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     2041 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     2101 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     2161 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     2221 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     2281 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     2341 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     2401 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2641 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2941 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3121 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3181 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3241 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3301 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactg
     3361 t
//