LOCUS       pICSL50049              2407 bp ds-DNA     circular     06-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..174
                     /label="HF_3C tag"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             144..167
                     /label="FLAG Tag"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="DYKDDDDK"
     promoter        293..397
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(388..468)
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             556..612
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             619..1407
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1581..2169
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2352..2401
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
ORIGIN
        1 ggtctcattc gGGTTCCGGA AGAGGATCGC ATCACCATCA CCATCACGAT TATGATATTC
       61 CAACTACTGC TAGCGGCCTG GAAGTTCTGT TTCAGGGCCC AGACTACAAA GACCATGACG
      121 GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTGA gctttgagac
      181 cacgtggctc ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      241 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      301 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      361 ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag
      421 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      481 ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
      541 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
      601 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
      661 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
      721 ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg
      781 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
      841 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
      901 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
      961 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     1021 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     1081 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     1141 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1201 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1261 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     1321 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa
     1381 ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt
     1441 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1501 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1561 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1621 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1681 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1741 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1801 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1861 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1921 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1981 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2041 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2101 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2161 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2221 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2281 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2341 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2401 tcactgt
//