LOCUS       pICSL50051              2425 bp ds-DNA     circular     06-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    15..98
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    105..125
                     /label="TEV"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    135..182
                     /label="SpyTag003"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        311..415
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(406..486)
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             574..630
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             637..1425
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1599..2187
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2370..2419
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
ORIGIN
        1 ggtctcattc gggcTCGGAT TATAAGGACC ATGACGGAGA CTATAAGGAC CATGACCTCG
       61 ACGCTGCAGC AGCGGATTAT AAGGACGATG ACGATAAGtc gggcGAGAAT TTGTATTTTC
      121 AGGGTTCGgg ctcgcgtggc gtgcctcata tcgtgatggt ggacgcctac aagcgttaca
      181 agTTTtaagc tttgagacca cgtggctctt cagtggacga aagggcctcg tgatacgcct
      241 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      301 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      361 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg
      421 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
      481 tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag
      541 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
      601 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
      661 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
      721 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt
      781 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
      841 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
      901 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
      961 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     1021 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     1081 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     1141 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     1201 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     1261 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     1321 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     1381 tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa
     1441 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1501 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1561 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1621 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1681 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1741 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1801 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1861 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1921 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1981 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2041 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2101 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2161 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2221 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2281 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2341 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     2401 cgttggccga ttcattaatc actgt
//