LOCUS       pICSL50053              2821 bp ds-DNA     circular     06-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     MIsc._Feature   8..11
                     /label="TTCG 4bp overhang"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    8..11
                     /label="Geneious type: Editing History Insertion"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Geneious type: Editing History Insertion"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     gene            12..557
                     /label="SNAP26f gene"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /gene="SNAP26f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     source          12..557
                     /label="source Cloning vector pSNAPf"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /organism="Cloning vector pSNAPf"
                     /mol_type="other DNA"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             12..557
                     /label="SNAP26f CDS"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_fwdcolor="#e9d024"
                     /note="optimized for mammalian expression; no stop codon;possible alternative start 774"
                     /gene="SNAP26f"
                     /product="O-6-methylguanine-DNA methyltransferase"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEVPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAVKEWLLAHEGHRLGKPGLG"
     CDS             558..581
                     /label="HELLFIRE"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="DYKDDDDK"
     CDS             558..581
                     /label="C terminal Luciferase tag - whole coding sequence "
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="DYKDDDDK"
     misc_feature    558..588
                     /label="SNAP26f CDS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Geneious type: Editing History Insertion"
                     /label="SNAP26f CDS(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             582..584
                     /label="C terminal 6xHA (Human influenza hemagglutinin) ta"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="*"
     MIsc._Feature   585..588
                     /label="GCTT 4bp overhang"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        707..811
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(802..882)
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             970..1026
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             1033..1821
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1995..2583
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2766..2815
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
ORIGIN
        1 ggtctcattc gatggacaaa gactgcgaaa tgaagcgcac caccctggat agccctctgg
       61 gcaagctgga actgtctggg tgcgaacagg gcctgcaccg tatcatcttc ctgggcaaag
      121 gaacatctgc cgccgacgcc gtggaagtgc ctgccccagc cgccgtgctg ggcggaccag
      181 agccactgat gcaggccacc gcctggctca acgcctactt tcaccagcct gaggccatcg
      241 aggagttccc tgtgccagcc ctgcaccacc cagtgttcca gcaggagagc tttacccgcc
      301 aggtgctgtg gaaactgctg aaagtggtga agttcggaga ggtcatcagc tacagccacc
      361 tggccgccct ggccggcaat cccgccgcca ccgccgccgt gaaaaccgcc ctgagcggaa
      421 atcccgtgcc cattctgatc ccctgccacc gggtggtgca gggcgacctg gacgtggggg
      481 gctacgaggg cgggctcgcc gtgaaagagt ggctgctggc ccacgagggc cacagactgg
      541 gcaagcctgg gctgggtgac tacaaggatg acgatgacaa gtgagctttg agaccacgtg
      601 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      661 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      781 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
      841 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
      901 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
      961 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1021 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     1081 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     1141 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
     1201 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1261 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1321 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1381 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1441 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     1501 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     1561 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     1621 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     1681 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     1741 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     1801 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     1861 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1921 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1981 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2041 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2101 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2161 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2221 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2281 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2341 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2401 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2461 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2521 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2581 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2641 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2701 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2761 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactg
     2821 t
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