LOCUS       pICSL50065               2737 bp    DNA     circular UNA 25-MAY-2023
DEFINITION  pL0M-CT-3xGly4Ser-GAL4 (GeneArt codon-optimized for Nicotiana
            benthamiana).
ACCESSION   urn.local...3zq-gfp633w
VERSION     urn.local...3zq-gfp633w
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     terminator      1..95
                     /Note="recombinase family protein terminator"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="recombinase family protein terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:46-g09vqkf"">recombinase family protein
                     terminator</a>"
                     /label="recombinase family protein terminator"
     promoter        96..200
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="bla promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:3az-aosb68k"">bla promoter</a>"
                     /label="bla promoter"
     CDS             201..1211
                     /name="aadA CDS"
                     /gene="aadA"
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /note="confers resistance against aminoglycosides
                     (PF01909)"
                     /transl_table=11
                     /modified_by="jkourelis"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="AadA1 CDS"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; aadA CDS
                     (<a href=""urn:local:.:37s-aosb68i"">AadA1 CDS</a>)"
                     /label="aadA CDS"
     promoter        complement(301..329)
                     /gene="intI1"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="IntI promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; int1
                     promoter (<a href=""urn:local:.:j3-bjsx83a"">IntI
                     promoter</a>)"
                     /label="int1 promoter"
     terminator      1212..1359
                     /Note="Beta-lactamase TEM terminator"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="bla terminator"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1rb-fwqz7ed"">bla terminator</a>"
                     /label="bla terminator"
     terminator      complement(1361..1399)
                     /name="Tndr"
                     /note="rho independent terminator"
                     /modified_by="jkourelis"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ori rep origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tndr (<a
                     href=""urn:local:.:3c2-aosb68l"">ori rep origin</a>)"
                     /label="Tndr"
     rep_origin      1402..1970
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication; ori"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ori rep origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:3c2-aosb68l"">ori rep origin</a>"
                     /label="ori rep origin"
     misc_RNA        1418..1970
                     /name="RNA-II"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ori rep origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RNA-II
                     (<a href=""urn:local:.:3c2-aosb68l"">ori rep origin</a>)"
                     /label="RNA-II"
     terminator      complement(1419..1452)
                     /name="Tndr"
                     /note="rho independent terminator"
                     /modified_by="jkourelis"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ori rep origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Tndr (<a
                     href=""urn:local:.:3c2-aosb68l"">ori rep origin</a>)"
                     /label="Tndr"
     misc_RNA        complement(1421..1528)
                     /name="RNA-I"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ori rep origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RNA-I
                     (<a href=""urn:local:.:3c2-aosb68l"">ori rep origin</a>)"
                     /label="RNA-I"
     regulatory      1781..2159
                     /regulatory_class="other"
                     /note="ColE1 ori"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ColE1 ori regulatory"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:39n-aosb68j"">ColE1 ori regulatory</a>"
                     /label="ColE1 ori regulatory"
     misc_feature    2090..2153
                     /name="L-strand Y effector site"
                     /note="L-strand Y effector site"
                     /modified_by="jkourelis"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L-strand Y effector site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:370-aosb68h"">L-strand Y effector
                     site</a>"
                     /label="L-strand Y effector site"
     CDS             2222..2263
                     /created_by="kourelis"
                     /label="3xGly4Ser linker CDS"
     CDS             2264..>2707
                     /gene="GAL4"
                     /note="DNA-binding transcription factor required for
                     activating GAL genes; responds to galactose; repressed by
                     Gal80p and activated by Gal3p"
                     /transl_table=1
                     /modified_by="kourelis"
                     /label="GAL4 CDS"
     Region          2288..2404
                     /region_name="Zn_clus"
                     /note="Fungal Zn(2)-Cys(6) binuclear cluster domain;
                     pfam00172"
                     /db_xref="CDD:201056"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="GAL4-VP16-GR CDS"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Zn clus
                     (<a href=""urn:local:.:1h4x-ecqey6x"">GAL4-VP16-GR
                     CDS</a>)"
                     /label="Zn clus"
     Region          2411..2548
                     /region_name="ZIP_Gal4"
                     /note="Leucine zipper Dimerization domain of transcription
                     factor Gal4 and similar fungal proteins; cd14654"
                     /db_xref="CDD:271238"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="GAL4"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZIP Gal4
                     (<a href=""urn:local:.:hy-g8uqd46"">GAL4</a>)"
                     /label="ZIP Gal4"
     Region          2417..2479
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:271238"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="GAL4-VP16-GR CDS"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; coiled
                     coil (<a href=""urn:local:.:1h4x-ecqey6x"">GAL4-VP16-GR
                     CDS</a>)"
                     /label="coiled coil"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      241 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
      301 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
      361 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
      421 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      481 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      541 gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      601 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      661 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      721 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
      781 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
      841 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
      901 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
      961 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     1021 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     1081 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1141 cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag
     1201 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1261 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1321 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1501 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1741 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1981 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2041 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2101 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2161 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcattc
     2221 gggtggtgga tcaggtggtg gtggttctgg tggcggagga tctatgaagc ttctgtcctc
     2281 tattgagcag gcttgcgata tctgccggct gaagaaactg aagtgcagca aagaaaagcc
     2341 gaagtgcgct aagtgcctga agaacaattg ggagtgccgg tactctccta agaccaagag
     2401 atctcctttg accagggctc atcttaccga ggttgaatct aggcttgaga ggctcgagca
     2461 gctgttcctt ttgatctttc cacgtgagga cctggacatg atcctgaaga tggattccct
     2521 gcaggacatc aaggctctgc ttactggtct tttcgtgcag gacaacgtga acaaggatgc
     2581 tgtgactgat aggctggcta gcgttgagac tgatatgcct cttactcttc ggcagcacag
     2641 gatctctgct acctcttcat ctgaggaaag cagcaacaag ggtcagagac agcttaccgt
     2701 gtcttaggct ttgagaccac gaagtggctc ttcagtg
//

