LOCUS       pICSL50067              2938 bp ds-DNA     circular     06-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..705
                     /label="mScarlet3"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="mScarlet3"
                     /label="mScarlet3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     promoter        824..928
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(919..999)
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             1087..1143
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             1150..1938
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2112..2700
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2883..2932
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
ORIGIN
        1 ggtctcattc gATGGATTCT ACCGAGGCCG TGATCAAAGA ATTCATGCGG TTCAAGGTGC
       61 ACATGGAAGG CTCTATGAAT GGCCACGAGT TCGAGATCGA AGGTGAAGGT GAGGGTAGAC
      121 CTTATGAGGG TACTCAGACC GCTAAGCTGA GGGTTACAAA AGGTGGTCCT CTGCCGTTCT
      181 CTTGGGATAT TTTGAGCCCG CAGTTCATGT ACGGCTCTCG GGCTTTTACA AAGCACCCTG
      241 CTGATATCCC TGACTACTGG AAGCAGTCTT TCCCAGAGGG TTTCAAGTGG GAGCGTGTGA
      301 TGAATTTCGA GGATGGTGGT GCTGTTTCCG TGGCTCAGGA TACTTCTCTT GAGGATGGCA
      361 CCCTGATCTA CAAGGTGAAG CTTCGGGGTA CTAACTTCCC TCCTGATGGT CCTGTGATGC
      421 AGAAAAAGAC CATGGGCTGG GAAGCTTCTA CTGAGAGGCT TTACCCTGAG GACGTTGTGC
      481 TGAAGGGCGA TATTAAGATG GCTCTGAGGC TTAAGGATGG CGGCAGATAC CTTGCTGATT
      541 TCAAGACTAC CTACCGGGCC AAGAAACCTG TTCAAATGCC TGGCGCTTTC AACATCGATC
      601 GGAAGCTGGA TATCACCAGC CACAACGAGG ATTACACTGT GGTTGAGCAG TACGAGAGAT
      661 CTGTGGCTAG GCATTCTACT GGTGGTTCTG GTGGATCTTA Ggctttgaga ccacgtggct
      721 cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      781 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      841 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      901 gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac
      961 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     1021 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     1081 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     1141 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     1201 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     1261 gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg
     1321 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     1381 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     1441 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     1501 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     1561 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     1621 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     1681 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     1741 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     1801 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     1861 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc
     1921 accaaggtag tcggcaaata actgtcagac caagtttact catatatact ttagattgat
     1981 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2041 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2221 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2761 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2821 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2881 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactgt
//