LOCUS       pICSL50069              2929 bp ds-DNA     circular     06-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    8..696
                     /label="mStayGold"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="mStayGold"
                     /label="mStayGold"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     promoter        815..919
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(910..990)
                     /label="int (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             1078..1134
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             1141..1929
                     /label="aadA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2103..2691
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2874..2923
                     /label="lacI (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.6"
                     /fragment="True"
                     /other="CDS"
                     /translation="APNTQTASPRALADSL"
ORIGIN
        1 ggtctcattc gATGGTGTCT ACCGGTGAGG AACTTTTCAC TGGTGTGGTG CCTTTCAAGT
       61 TCCAGCTTAA GGGCACCATC AACGGCAAGT CTTTCACCGT TGAAGGTGAA GGTGAGGGCA
      121 ATTCTCATGA GGGTTCTCAC AAGGGAAAGT ACGTGTGTAC CTCTGGCAAG CTGCCTATGT
      181 CTTGGGCTGC TCTTGGTACT TCTTTCGGCT ACGGCATGAA GTACTACACT AAGTACCCAA
      241 GCGGCCTGAA GAACTGGTTC CATGAAGTTA TGCCTGAGGG CTTCACCTAC GATAGGCACA
      301 TCCAGTACAA AGGCGACGGT AGCATTCATG CTAAGCACCA GCATTTCATG AAGAACGGCA
      361 CCTACCACAA CATCGTTGAG TTCACCGGCC AGGACTTCAA AGAGAACTCT CCTGTTCTGA
      421 CCGGCGATAT GGATGTGTCT TTGCCTAATG AGGTGCAGCA CATCCCTATC GATGATGGTG
      481 TTGAGTGCAC TGTGACCCTT CAGTACCCTC TGCTTTCCGA TGAGTCTAAG TGCGTTGAGG
      541 CTTACCAGAA CACCATTATC AAGCCGCTTC ACAACCAGCC TGCTCCAGAT GTTCCATTCC
      601 ACTGGATTAG GAAGCAGTAC ACCCAGAGCA AGGATGACAC TGAAGAGAGG GATCACATCA
      661 TCCAGAGCGA GACTCTTGAG GCTCACCTTT AGgctttgag accacgtggc tcttcagtgg
      721 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      781 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      901 atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
      961 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttttggggta
     1021 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     1081 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     1141 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     1201 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     1261 ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     1321 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     1381 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     1441 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     1501 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     1561 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     1621 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     1681 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     1741 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     1801 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     1861 tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta
     1921 gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2221 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2461 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2701 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2761 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2821 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     2881 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactgt
//