LOCUS       pICSL12001              2703 bp ds-DNA     circular     07-AUG-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     repeat_region   complement(749..760)
                     /label="AAV2 ITR (fragment)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
     Misc._feature   837..840
                     /label="GGAG 4bp overhang"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     Promoter        841..1241
                     /label="AtPSBQ promoter from At4g21280"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /vntifkey=29
                     /label="AtPSBQ promoter (At4g21280)"
     5'_UTR          1242..1285
                     /label="AtPSBQ 5' UTR from At4g21280"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /vntifkey=52
                     /label="AtPSBQ 5' UTR (At4g21280)"
     Misc._feature   1291..1294
                     /label="AATG 4bp overhang"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        1416..1520
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1511..1591)
                     /label="INT2_ECOLX (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="92.6"
                     /match_length="8.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MKTATAPLPPLRSVKVLDQLRERILFL"
     CDS             1679..1735
                     /label="GmR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="96.5"
                     /match_length="10.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MLWSSNDVTQQGSRPKTKL"
     CDS             1742..2530
                     /label="S3AD_ECOLX"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 GATTCTCAAG ATCTTAGAGA ATCAAATCGA AAACCTTCTC AATCGAAGTC GAGAAACTAG
      901 GATTCTTACT TTTTCCTAAT TTGGGGATTT TTGTGTGTGT GTAAGTGTGT GACTGAAGCA
      961 GGAGCCTGTA GTTTCGAAAT TCGAACGATT TTGAAATTAG ATGTGGCGGA CTTCTGGAAT
     1021 TATCATAACG TTATCAATCA ATAAAGTATT TAAACATATG GGCTAACTTT GGGCCTACAA
     1081 TTCGGCCCAA ATGTTTAAAT AATCTTGCCA CGTGGCTTTA AGGCGGACAA AAATGACACG
     1141 TGGTTTTATA TGAGTGGCTA CAACCATATC CAAGTTATCT AAAGTACAGA TAAGGCGCTC
     1201 TGTGTCGGAG AATGAAACCA ACCAATCATA TAAGCATAAC CAATAATCAT CGGCTTCAAT
     1261 TTTCGTCTTG AGAAAGAAAA ACAAAATGGC aatgtgagac cacgaagtgg ctcttcagtg
     1321 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1381 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1441 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1501 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     1561 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt
     1621 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     1681 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     1741 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     1801 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     1861 cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     1921 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     1981 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2041 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2101 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2161 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2221 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2281 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2341 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2401 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     2461 ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt
     2521 agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2581 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2641 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2701 ttc
//